From 4bf5986a2493ab724891ef5c50b9d8aa1630a713 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Thu, 12 Feb 2015 14:53:43 -0200 Subject: [PATCH 01/40] Update README.md --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 70f6aa933bffc..229e547715cae 100644 --- a/README.md +++ b/README.md @@ -1,2 +1,2 @@ -# BioHackAcademy.github.io -Waag Society's BioHack Academy Syllabus +# BioHackAcademyPTBR.github.io +Syllabus do BioHack Academy da Waag Society, em bom português! From 27747b032d22b9383ec4ce0af3df7a5abe5da455 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Thu, 12 Feb 2015 14:55:22 -0200 Subject: [PATCH 02/40] Update README.md --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 229e547715cae..ba0a218401fb0 100644 --- a/README.md +++ b/README.md @@ -1,2 +1,2 @@ # BioHackAcademyPTBR.github.io -Syllabus do BioHack Academy da Waag Society, em bom português! +Ementa do BioHack Academy da Waag Society, em bom português! From ddc8c6e32454966a7148ac837cedc27ab0e837f5 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Thu, 12 Feb 2015 15:00:11 -0200 Subject: [PATCH 03/40] Update _config.yml --- _config.yml | 7 ++++--- 1 file changed, 4 insertions(+), 3 deletions(-) diff --git a/_config.yml b/_config.yml index db3910cbfa32b..78c9f1177458b 100644 --- a/_config.yml +++ b/_config.yml @@ -1,8 +1,9 @@ # Site settings -title: Biohack Academy Syllabus -email: pieter@waag.org +title: Ementa Biohack Academy +email: info@garagemfablab.com description: > # this means to ignore newlines until "baseurl:" - Waag Society's first Biohack Academy is an intense 10 week bioreactor hacking course held in the Amsterdam Fablab and Open Wetlab. + O primeiro BioHack Academy da Waag Society's é um curso intenso de 10 semanas sobre como construir e hackaear um biorreator com ferramentas de fabricação digital, conhecimento aberto e espírito DIY! + Será ministrado no Open WetLab do FabLab Amsterdam e em outros FabLabs pelo mundo, como no Garagem FabLab em SP e Olabi no RJ. baseurl: "" # the subpath of your site, e.g. /blog/ url: "http://code.waag.org" # the base hostname & protocol for your site twitter_username: waag From 5537c8b5b8d370b245fff277a9522bd10ad9ded4 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Thu, 12 Feb 2015 15:04:52 -0200 Subject: [PATCH 04/40] Update about.md --- about.md | 9 +++++---- 1 file changed, 5 insertions(+), 4 deletions(-) diff --git a/about.md b/about.md index 217f03a6d5bd0..3f37cbdf1e19f 100644 --- a/about.md +++ b/about.md @@ -4,12 +4,13 @@ title: About permalink: /about/ --- -Biohack Academy is an education program of Waag Society Amsterdam. You can find more information on [waag.org/biohackacademy](http://www.waag.org/biohackacademy) +O Biohack Academy é um programa educacional da Waag Society de Amsterdam. Você pode encontrar mais infos em [waag.org/biohackacademy](http://www.waag.org/biohackacademy) ![Waag Society Open Wetlab]({{ site.baseurl }}/biofactory/graphics/WS_openwetlab_logo.png) -This site was made using the base Jekyll theme. You can find out more info about customizing this Jekyll theme, as well as basic Jekyll usage documentation at [jekyllrb.com](http://jekyllrb.com/) +Este site foi criado usando como base o tema Jekyll. Você pode encontrar mais informações sobre como customizar esse tema, assim como a documentação básica sobre seu uso em [jekyllrb.com](http://jekyllrb.com/) -You can find the source code for the Jekyll new theme at: [github.com/jglovier/jekyll-new](https://github.com/jglovier/jekyll-new) +Aqui você encontra o código-fonte do Jekyll New Theme: +[github.com/jglovier/jekyll-new](https://github.com/jglovier/jekyll-new) -You can find the source code for Jekyll at [github.com/jekyll/jekyll](https://github.com/jekyll/jekyll) +E aqui você encontra o código-fonte do Jekyll [github.com/jekyll/jekyll](https://github.com/jekyll/jekyll) From f87740dfbc426ee778ea30e189142edfecbfcecc Mon Sep 17 00:00:00 2001 From: Eduardo Date: Thu, 12 Feb 2015 15:17:53 -0200 Subject: [PATCH 05/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/1/contamination-test.md | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/biofactory/class/1/contamination-test.md b/biofactory/class/1/contamination-test.md index 694a89611e7f6..cba70cee0a32b 100644 --- a/biofactory/class/1/contamination-test.md +++ b/biofactory/class/1/contamination-test.md @@ -7,8 +7,10 @@ permalink: /biofactory/class/1-incubator/contamination-test/ ## Goal Microorganisms are always around and ready to contaminate your carefully grown cultures. In this practical we will determine what common airborne contaminents look like. +Os microorganismos estão sempre por perto e prontos para contaminar as suas culturas cuidadosamente cultivadas. Através deste teste, você vai aprender como surgem os contaminantes transportados pelo ar mais comuns. ## Materials +## Materiais * 1 Peptone agar (PA) plate, favourable to bacteria * 1 Malt agar (MA) plate, favourable to yeast and fungi @@ -21,4 +23,4 @@ Microorganisms are always around and ready to contaminate your carefully grown c 4. Incubate at 30° C for 24h. 5. Inspect your plates and look at them under the microscope. -Back to [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) +Back to [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) \ No newline at end of file From 2314d1e9d04ee428e2d7cb45d11d9c2697f61ec9 Mon Sep 17 00:00:00 2001 From: Eduardo Date: Thu, 12 Feb 2015 15:18:06 -0200 Subject: [PATCH 06/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/1/contamination-test.md | 22 ++++++++++------------ 1 file changed, 10 insertions(+), 12 deletions(-) diff --git a/biofactory/class/1/contamination-test.md b/biofactory/class/1/contamination-test.md index cba70cee0a32b..07e4a72328d63 100644 --- a/biofactory/class/1/contamination-test.md +++ b/biofactory/class/1/contamination-test.md @@ -4,23 +4,21 @@ title: Contamination Test permalink: /biofactory/class/1-incubator/contamination-test/ --- -## Goal +## Objetivo -Microorganisms are always around and ready to contaminate your carefully grown cultures. In this practical we will determine what common airborne contaminents look like. Os microorganismos estão sempre por perto e prontos para contaminar as suas culturas cuidadosamente cultivadas. Através deste teste, você vai aprender como surgem os contaminantes transportados pelo ar mais comuns. -## Materials ## Materiais -* 1 Peptone agar (PA) plate, favourable to bacteria -* 1 Malt agar (MA) plate, favourable to yeast and fungi +* 1 placa de Peptone Agar (PA), favorável às bactérias +* 1 placa de Agar de malte (MA), favorável à leveduras e fungos -## Method +## Metodologia -1. Find an interesting spot in the building. -2. Remove the lid from the plates and leave the plates for 30 minutes. -3. Collect the plates. -4. Incubate at 30° C for 24h. -5. Inspect your plates and look at them under the microscope. +1. Ache um local interessante no seu entorno. Pode ser uma sala, um banheiro… +2. Remova a tampa das placas e deixe as placas em repouso durante 30 minutos. +3. Recolha as placas. +4. Incube-as a 30°C durante 24h. +5. Inspecione suas placas e olhe-as com um microscópio. -Back to [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) \ No newline at end of file +De volta ao [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) \ No newline at end of file From 0150e64d95cb61b8c8c674bb111e98beb955ee4c Mon Sep 17 00:00:00 2001 From: Eduardo Date: Thu, 12 Feb 2015 16:00:21 -0200 Subject: [PATCH 07/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- .../class/1/isolation of yoghurt bacteria.md | 128 +++++++++--------- 1 file changed, 64 insertions(+), 64 deletions(-) diff --git a/biofactory/class/1/isolation of yoghurt bacteria.md b/biofactory/class/1/isolation of yoghurt bacteria.md index 8f608ec25ec94..5de3baeba0354 100644 --- a/biofactory/class/1/isolation of yoghurt bacteria.md +++ b/biofactory/class/1/isolation of yoghurt bacteria.md @@ -4,67 +4,67 @@ title: Isolation of Yoghurt bacteria permalink: /biofactory/class/1-incubator/yoghurt-bacteria-isolation/ --- -## Goal - -When lactose is converted into lactic acid by bacteria, milk is converted into yoghurt. In this practical we will isolate the most common agents: *Lactobacillus* and *Streptococcus*. - -## Materials - -**Step 1** - -* 50 mL [Ringer's Solution](/biofactory/annex/cultivation-media/ringers-solution/) -* 5 test tubes -* 6 [MRS Agar](/biofactory/annex/cultivation-media/mrs-agar/) plates -* 1 [Malt Agar](/biofactory/annex/cultivation-media/malt-agar/) plate -* pH indicator paper -* commercial yoghurt, such as Actimel -* Glass spatula -* Inoculation loop -* 100 mL beaker glass with some 95% ethanol -* Gas burner - -**Step 2** - -* 4 [MRS Agar](/biofactory/annex/cultivation-media/mrs-agar/) plates -* 2 [Malt Agar](/biofactory/annex/cultivation-media/malt-agar/) plates - -**Step 3** - -* Microscope -* Microscope slides and cover slides -* Gram and Loeffler staining - -## Method Step 1 - -1. Label the 5 test tubes: -1, -3, -5, -7 and -9 -2. Add 9 mL Ringer's solution to tube -1 -3. Add 9.9 mL Ringer's solution to tube -3, -5, -7 and -9 -4. Add 1 g of yoghurt to tube -1 and vortex/mix -5. With the use of sterile pipette transfer 0.1 mL from the -1 tube to the -3 tube. Vortex / mix. -6. Transfer 0.1 mL from the -3 tube to the -5 tube. Vortex / mix. -7. Transfer 0.1 mL from the -5 tube to the -7 tube. Vortex / mix. -8. Transfer 0.1 mL from the -7 tube to the -9 tube. Vortex / mix. -9. Label 5 MRS plates with the same labels: -1, -3, -5, -7 and -9 -10. Starting with the largest dilution (-9) transfer 0.25 mL from the test tube to the -9 plate and spread it using the glass spatula that has been sterilized by dipping it in alcohol and torching it. Continue with the next tube (-7) and so on. -11. Heat up the inoculation loop to red hot, let it cool down, dip it in the yoghurt and streak it on the MRS plate. -12. Once more, heat up the inoculation loop to red hot, let it cool down, dip it in the yoghurt and streak it on the MA plate. -13. Incubate plates at 30° C. -14. Measure the pH of the yoghurt using the pH indicator paper. - -## Method Step 2 -After about one week: - -1. Take a look at your plates. -2. Select a plate with clear individual colonies. -3. Count the colonies. -4. Streak one bacterial colony on each fresh MRS plate. -5. Streak a yeast contamination on a clean MA plate. -6. Incubate the plates at 30° C. - -## Method Step 3 -After about one more week: - -1. Perform a Gram staining on the bacteria and a Loeffler staining on the yeast. -2. Take a look at the cultures under the microscope. - -Back to [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) +## Objetivo + +Quando se converte a lactose em ácido láctico atrevés de bactérias, o leite é convertido em iogurte. Neste teste, vamos isolar os agentes mais comuns: *Lactobacillus* e *Streptococcus*. + +## Materiais + +**Passo 1** + +* 50 mL de Solução de Ringer [Ringer's Solution](/biofactory/annex/cultivation-media/ringers-solution/) +* 5 tubos de ensaio +* 6 placas de [MRS Agar](/biofactory/annex/cultivation-media/mrs-agar/) +* 1 placa de [Agar de Malte](/biofactory/annex/cultivation-media/malt-agar/) +* Papel indicador de pH +* Iogurte encontrado no mercado, como Activia +* Espátula de vidro +* Alça de inoculação +* Becker de vidro de 100 ml com um pouco de alcool etílico a 95% +* Fogareiro + +**Passo 2** + +* 4 placas [MRS Agar](/biofactory/annex/cultivation-media/mrs-agar/) +* 2 placas de [Agar de malte] (/biofactory/annex/cultivation-media/malt-agar/) + +**Passo 3** + +* Microscópio +* Lâminas de microscópio e lâminas de cobertura +* Coloração de Gram e Loeffler + +## Metodologia Passo 1 + +1. Etiquete os cinco tubos de ensaio: -1, -3, -5, -7 e -9 +2. Adicione 9 mL de solução de Ringer no tubo -1 +3. Adicione 9,9 mL de solução de Ringer nos tubos -3, -5, -7 e -9 +4. Adicione 1 g de iogurte no tubo -1 e vortex/mix +5. Com uma pipeta estéril, transfira 0,1 mL de solução do tubo -1 ao tubo -3. Vortex/mix. +6. Transfira 0,1 mL de solução do tubo -3 ao tubo -5. Vortex/mix. +7. Transfira 0,1 mL de solução do tubo -5 ao tubo -7. Vortex/mix. +8. Transfira 0,1 mL de solução do tubo -7 ao tubo -9. Vortex/mix. +9. Etiquete as cinco placas MRS com as mesmas etiquetas: -1, -3, -5, -7 and -9 +10. Começando com a maior diluição (-9), transfira 0,25 mL de solução do tubo de ensaio para a placa -9 e espalhe-a utilizando a espátula de vidro (que foi esterilizada mergulhando-la no álcool e incendiando-a). Continue com o tubo seguinte (-7) e assim por diante. +11. Aqueça a alça de inoculação ate que fique vermelha, deixe esfriar, mergulhe-a no iogurte e estrie na placa MRS. +12. Mais uma vez, aqueça a alça de inoculação até que seja vermelha, deixe esfriar, mergulhá-la no iogurte e estrie na placa de MA. +13. Incube as placas a 30 °C. +14. Meça o pH do iogurte usando o papel indicador de pH. + +## Metodologia Passo 2 +Após cerca de uma semana: + +1. Dê uma olhada as suas placas. +2. Selecione uma placa com claras colônias individuais. +3. Conte as colônias. +4. Estrie uma colônia de bactérias em cada placa fresca de MRS. +5. Estrie uma contaminação de fermento numa placa limpa de MA. +6. Incube as placas a 30 °C. + +## Metodologia Passo 3 +Após mais uma semana: + +1. Realize uma coloração de Gram sobre as bactérias e uma coloração Loeffler sobre a levedura. +2. Dê uma olhada nas culturas com um microscópio. + +De volta ao [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) From f9fda952b3a795e3ec25f0067a47ce480acdb87e Mon Sep 17 00:00:00 2001 From: Eduardo Date: Thu, 12 Feb 2015 16:01:13 -0200 Subject: [PATCH 08/40] Typo --- biofactory/class/1/isolation of yoghurt bacteria.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/biofactory/class/1/isolation of yoghurt bacteria.md b/biofactory/class/1/isolation of yoghurt bacteria.md index 5de3baeba0354..bbc46199e42f7 100644 --- a/biofactory/class/1/isolation of yoghurt bacteria.md +++ b/biofactory/class/1/isolation of yoghurt bacteria.md @@ -6,7 +6,7 @@ permalink: /biofactory/class/1-incubator/yoghurt-bacteria-isolation/ ## Objetivo -Quando se converte a lactose em ácido láctico atrevés de bactérias, o leite é convertido em iogurte. Neste teste, vamos isolar os agentes mais comuns: *Lactobacillus* e *Streptococcus*. +Quando se converte a lactose em ácido láctico através de bactérias, o leite é convertido em iogurte. Neste teste, vamos isolar os agentes mais comuns: *Lactobacillus* e *Streptococcus*. ## Materiais From 15f0e2b0c1c244ef48493aa088cce33d8b5f06f3 Mon Sep 17 00:00:00 2001 From: Eduardo Date: Thu, 12 Feb 2015 20:41:33 -0200 Subject: [PATCH 09/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- .../class/1/isolation of beer yeasts.md | 128 +++++++++--------- 1 file changed, 64 insertions(+), 64 deletions(-) diff --git a/biofactory/class/1/isolation of beer yeasts.md b/biofactory/class/1/isolation of beer yeasts.md index 9ad233e40e902..2c9a7584d2448 100644 --- a/biofactory/class/1/isolation of beer yeasts.md +++ b/biofactory/class/1/isolation of beer yeasts.md @@ -4,67 +4,67 @@ title: Isolation of Beer yeasts permalink: /biofactory/class/1-incubator/beer-yeasts-isolation/ --- -## Goal - -Especially famous Belgian beers continue to ferment in the bottle. During fermentation the sugars are converted into ethanol. In this practical we will isolate the yeasts. - -## Materials - -**Step 1** - -* 50 mL [Ringer's Solution](/biofactory/annex/cultivation-media/ringers-solution/) -* 5 test tubes -* 1 [MRS Agar](/biofactory/annex/cultivation-media/mrs-agar/) plate -* 6 [Malt Agar](/biofactory/annex/cultivation-media/malt-agar/) plate -* pH indicator paper -* Beer, for example Duvel or Grimbergen -* Glass spatula -* Inoculation loop -* 100 mL beaker glass with some 95% ethanol -* Gas burner - -**Step 2** - -* 2 [MRS Agar](/biofactory/annex/cultivation-media/mrs-agar/) plates -* 4 [Malt Agar](/biofactory/annex/cultivation-media/malt-agar/) plates - -**Step 3** - -* Microscope -* Microscope slides and cover slides -* Gram and Loeffler staining - -## Method Step 1 - -1. Label the 5 test tubes: -1, -3, -5, -7 and -9 -2. Add 9 mL Ringer's solution to tube -1 -3. Add 9.9 mL Ringer's solution to tube -3, -5, -7 and -9 -4. Add 1 g of yoghurt to tube -1 and vortex/mix -5. With the use of sterile pipette transfer 0.1 mL from the -1 tube to the -3 tube. Vortex / mix. -6. Transfer 0.1 mL from the -3 tube to the -5 tube. Vortex / mix. -7. Transfer 0.1 mL from the -5 tube to the -7 tube. Vortex / mix. -8. Transfer 0.1 mL from the -7 tube to the -9 tube. Vortex / mix. -9. Label 5 MA plates with the same labels: -1, -3, -5, -7 and -9 -10. Starting with the largest dilution (-9) transfer 0.25 mL from the test tube to the -9 plate and spread it using the glass spatula that has been sterilized by dipping it in alcohol and torching it. Continue with the next tube (-7) and so on. -11. Heat up the inoculation loop to red hot, let it cool down, dip it in the beer and streak it on the MA plate. -12. Once more, heat up the inoculation loop to red hot, let it cool down, dip it in the beer and streak it on the MRS plate. -13. Incubate plates at 30° C. -14. Measure the pH of the beer using the pH indicator paper. - -## Method Step 2 -After about one week: - -1. Take a look at your plates. -2. Select a plate with clear individual colonies. -3. Count the colonies. -4. Streak one yeast colony on each fresh MA plate. -5. Streak a bacterial contamination on a clean MRS plate. -6. Incubate the plates at 30° C. - -## Method Step 3 -After about one more week: - -1. Perform a Gram staining on the bacteria and a Loeffler staining on the yeast. -2. Take a look at the cultures under the microscope. - -Back to [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) +## Objetivo + +As especialmente famosas cervejas belgas continuam a fermentar na garrafa. Durante a fermentação os açúcares são convertidos em etanol. Neste experimento, vamos isolar as leveduras. + +## Materiais + +**Passo 1** + +* 50 mL de [Solução de Ringer](/biofactory/annex/cultivation-media/ringers-solution/) +* 5 tubos de ensaio +* 1 Placa de [Agar MRS](/biofactory/annex/cultivation-media/mrs-agar/) +* 6 Placas de [Agar de Malte](/biofactory/annex/cultivation-media/malt-agar/) plate +* Papel indicador de pH +* Cerveja, por exemplo Duvel ou Grimbergen. Se for caro demais, vale uma Erdinger…;) +* Espátula de vidro +* Alça de inoculação +* Becker de vidro de 100 ml com um pouco de alcool etílico a 95% +* Fogareiro + +**Passo 2** + +* 2 placas de [Agar MRS](/biofactory/annex/cultivation-media/mrs-agar/) +* 4 placas de [Agar de Malte](/biofactory/annex/cultivation-media/malt-agar/) + +**Passo 3** + +* Microscopio +* Lâminas de microscópio e lâminas de cobertura +* Coloração de Gram e Loeffler + +## Metodologia Passo 1 + +1. Etiquete os cinco tubos de ensaio: -1, -3, -5, -7 e -9 +2. Adicione 9 mL de Solução de Ringer no tubo -1 +3. Adicione 9,9 mL de Solução de Ringer nos tubos -3, -5, -7 e -9 +4. Adicione 1 g de iogurte no tubo -1 e vortex/mix +5. Com uma pipeta estéril, transfira 0,1 mL de solução do tubo -1 ao tubo -3. Vortex/mix. +6. Transfira 0,1 mL de solução do tubo -3 ao tubo -5. Vortex/mix. +7. Transfira 0,1 mL de solução do tubo -5 ao tubo -7. Vortex/mix. +8. Transfira 0,1 mL de solução do tubo -7 ao tubo -9. Vortex/mix. +9. Etiquete 5 placas MA da mesma maneira: -1, -3, -5, -7 e -9 +10.Começando com a maior diluição (-9), transfira 0,25 mL de solução do tubo de ensaio para a placa -9 e espalhe-a utilizando a espátula de vidro (que foi esterilizada mergulhando-la no álcool e incendiando-a). Continue com o próximo tubo (-7) e assim por diante. +11.Aqueça a alça de inoculação ate que fique vermelha, deixe esfriar, mergulhe-a na cerveja e estrie na placa MRS. +12.Mais uma vez, aqueça a alça de inoculação até que fique vermelha, deixe esfriar, mergulhe-a na cerveja e estrie na placa de MA. +13.Incube as placas a 30 °C. +14.Meça o pH da cerveja usando o papel indicador de pH. + +## Metodologia Passo 2 +Após uma semana: + +1. Dê uma olhada as suas placas. +2. Selecione uma placa com claras colônias individuais. +3. Conte as colônias. +4. Estrie uma colônia de fermentos em cada placa fresca de MA. +5. Estrie uma contaminação de bacterias numa placa limpa de MRS. +6. Incube as placas a 30 °C. + +## Metodologia Passo 3 +Após mais uma semana: + +1. Realize uma coloração de Gram sobre as bactérias e uma coloração Loeffler sobre a levedura. +2. Dê uma olhada nas culturas com um microscópio. + +Voltar para [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) From 54a570fffcb7f1b5ea47622ffeabd13af7ec90fd Mon Sep 17 00:00:00 2001 From: Eduardo Date: Thu, 12 Feb 2015 20:43:42 -0200 Subject: [PATCH 10/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/1/isolation of beer yeasts.md | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/biofactory/class/1/isolation of beer yeasts.md b/biofactory/class/1/isolation of beer yeasts.md index 2c9a7584d2448..d3d71b076030f 100644 --- a/biofactory/class/1/isolation of beer yeasts.md +++ b/biofactory/class/1/isolation of beer yeasts.md @@ -1,6 +1,6 @@ --- layout: page -title: Isolation of Beer yeasts +title: Isolando as Leveduras da Cerveja permalink: /biofactory/class/1-incubator/beer-yeasts-isolation/ --- @@ -46,10 +46,10 @@ As especialmente famosas cervejas belgas continuam a fermentar na garrafa. Duran 8. Transfira 0,1 mL de solução do tubo -7 ao tubo -9. Vortex/mix. 9. Etiquete 5 placas MA da mesma maneira: -1, -3, -5, -7 e -9 10.Começando com a maior diluição (-9), transfira 0,25 mL de solução do tubo de ensaio para a placa -9 e espalhe-a utilizando a espátula de vidro (que foi esterilizada mergulhando-la no álcool e incendiando-a). Continue com o próximo tubo (-7) e assim por diante. -11.Aqueça a alça de inoculação ate que fique vermelha, deixe esfriar, mergulhe-a na cerveja e estrie na placa MRS. -12.Mais uma vez, aqueça a alça de inoculação até que fique vermelha, deixe esfriar, mergulhe-a na cerveja e estrie na placa de MA. -13.Incube as placas a 30 °C. -14.Meça o pH da cerveja usando o papel indicador de pH. +11. Aqueça a alça de inoculação ate que fique vermelha, deixe esfriar, mergulhe-a na cerveja e estrie na placa MRS. +12. Mais uma vez, aqueça a alça de inoculação até que fique vermelha, deixe esfriar, mergulhe-a na cerveja e estrie na placa de MA. +13. Incube as placas a 30 °C. +14. Meça o pH da cerveja usando o papel indicador de pH. ## Metodologia Passo 2 Após uma semana: From db384d71b4981c4c4a34b88ef253755029a070ab Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Fri, 13 Feb 2015 11:17:42 -0200 Subject: [PATCH 11/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/1/incubator-materials.md | 17 ++++++++--------- 1 file changed, 8 insertions(+), 9 deletions(-) diff --git a/biofactory/class/1/incubator-materials.md b/biofactory/class/1/incubator-materials.md index 271269555f01d..cb9838a276ecf 100644 --- a/biofactory/class/1/incubator-materials.md +++ b/biofactory/class/1/incubator-materials.md @@ -1,21 +1,20 @@ --- layout: page -title: Incubator Materials +title: Materiais Incubadora permalink: /biofactory/class/1-incubator/incubator-materials/ --- -> PLEASE NOTE: as this is the first Bill of Materials of the Academy take note of the following: +> CONSIDERAÇÕES IMPORTANTES: como essa é a primeira BOM (Bill of Materials ou Lista de Materiais) do Curso, preste atenção ao seguinte: -1. The components on this list are the bare minimum you need to build an incubator and compatible with the incubator [3D design](/biofactory/class/1/Incubator-Sketchup.skp), [Fritzing wiring](/biofactory/class/1/Incubator-Fritzing.fzz) and [Arduino code](/biofactory/class/1/Incubator-Arduino.txt). Please feel free to deviate from this list and change the design accordingly. Once the classes started we will keep track of your hacks on your own documentation site. -2. The suppliers all ship to addresses in The Netherlands, and some also ship abroad. Alternative sources are listed on the [Suppliers](/suppliers/) page. Whenever you find a suitable alternative, please post it in the comments at the bottom of the page. -3. Check the stock level and shipping times of each component before ordering. If it takes more than two weeks, look for an alternative. -4. Some suppliers, such as Farnell, only ship to businesses by default. Contact their service department to order as an individual. +1. Os componentes dessa lista são o mínimo que você precisará para construir uma incubadora e que são compatíveis com o presente [Projeto 3D](/biofactory/class/1/Incubator-Sketchup.skp), [Diagrama Fritzing](/biofactory/class/1/Incubator-Fritzing.fzz) e o [código em Arduino](/biofactory/class/1/Incubator-Arduino.txt). Por favor sinta-se livre para fugir dessa lista e mudar seus desenhos e planos. Uma vez que as aulas começem, nós teremos o controle de seus "hacks" nas páginas de documentação. +2. Os fornecedores dessa lista enviam para todo o Brasil. Se você achar alguma alternativa melhor ou mais barata, por favor poste-a na área de comentários no final da página [Fornecedores](suppliers/). +3. Sempre cheque o tempo estimado de envio antes de comprar. Se não der tempo de chegar para a aula, procure alternativas. -## Bill of Materials +## Lista de Materiais -Needless to say, you will need an Arduino UNO or equivalent microcontroller. Most likely you have already bought one, as part of the [general preparations](/biofactory/preparations/). +Aqui você vai precisar de um microcontrolador Arduino UNO ou equivalente. Se você se preparou direitinho lendo a página sobre [Preparações](/biofactory/preparations/) você já está com o seu em mãos. -### Must have +### Devemos ter These are the parts you will need to assemble the incubator: From 5118b960a3118d3eabde2f88cd49969090e476c0 Mon Sep 17 00:00:00 2001 From: Eduardo Date: Fri, 13 Feb 2015 11:32:58 -0200 Subject: [PATCH 12/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/1/incubator-materials.md | 24 +++++++++++------------ 1 file changed, 12 insertions(+), 12 deletions(-) diff --git a/biofactory/class/1/incubator-materials.md b/biofactory/class/1/incubator-materials.md index cb9838a276ecf..645c7176e06b1 100644 --- a/biofactory/class/1/incubator-materials.md +++ b/biofactory/class/1/incubator-materials.md @@ -16,20 +16,20 @@ Aqui você vai precisar de um microcontrolador Arduino UNO ou equivalente. Se vo ### Devemos ter -These are the parts you will need to assemble the incubator: +Estas são as peças que você precisa para montar a incubadora: -|#|Amount|Description|Supplier NL|Cost| +|#|Quantidade|Descrição|Fornecedor BR|Custo| |-:|----:|:---------|:-------|---:| -|1|1|3mm MDF 95 x 45 cm|Local wood store, like [Houthandel Schmidt](https://www.google.com/maps/dir/Waag+Society,+Nieuwmarkt,+Amsterdam,+Netherlands/Houthandel+Schmidt,+Oudezijds+Achterburgwal+53,+1012+DB+Amsterdam,+Netherlands/@52.3732195,4.8971869,17z/data=!3m1!4b1!4m13!4m12!1m5!1m1!1s0x47c609b93deae857:0xa3c3b57e66c44946!2m2!1d4.900298!2d52.372807!1m5!1m1!1s0x47c609b901ad7703:0x6d511a1e0f5be9c2!2m2!1d4.89915!2d52.373417)|5.00| -|2|1|Expanded polystyrene (EPS)|[Praxis](https://www.praxis.nl/bouwmaterialen/isolatie/isolatie/isolatieplaat-eps-60-100-x-50-x-5cm-5-stuks/5322572)|7.99| -|4|1|3mm Acrylic sheet 25 x 40 cm|[Plexiglas.nl](http://www.plexiglas.nl/shop/plexiglas-helder-3-mm)|3.50| -|5|1|12V 80 mm Axial Fan|[EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=13244), [Farnell](http://nl.farnell.com/bisonic/sp802512l-03/fan-80x25mm-12vdc/dp/1832326)|8.99| -|6|1|Power switch|[EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=11596), [iPrototype](https://iprototype.nl/products/components/buttons-switches/rocker-switch-large)|0.95| -|7|1|Water proof temperature sensor (*1)|[HobbyElectronica](http://www.hobbyelectronica.nl/product/ds18b20-waterdicht/), [iPrototype](https://iprototype.nl/products/components/sensors/waterproof-tmp-sensor-10k)|2.95| -|8|1|4 section 7 segment display|[EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=8390), [iPrototype](https://iprototype.nl/products/components/led-lcd/led-bar-4xsegment-red)|1.95| -|9|1|MOSFET|[Farnell](http://nl.farnell.com/stmicroelectronics/stp36nf06l/mosfet-n-logic-to-220/dp/9935614?CMP=i-bf9f-00001000), [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=1279)|0.98| -|10|4|10K resistor|[Farnell](http://nl.farnell.com/te-connectivity/cfr16j10k/resistor-carbon-10k-0-25w-5/dp/2329474), [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=13492)|0.06| -|11|1|Diode|[iPrototype](https://iprototype.nl/products/components/overige/diode), [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=10717)|0.19| +|1|1|Chapa de MDF 3mm (cortada 600x400mm)|[Leroy Merlin](http://www.leroymerlin.com.br/mdf-2750-mmx1830-mmx3-mm-5004014-leo-madeiras_88365025)|75.00| +|2|1|Pacote (5un) placas Isopor 100x50x5cm|[Só Isopor](http://www.soisopor.com.br/?1.0.0.0,1170,pacote-placa-50mm-tipo-1.html)|30.00| +|4|1|Chapa de acrílico cristal 3mm (cortada 600x400mm)|[Garagem](-)|15.00| +|5|1|Cooler 80x80x25mm 12V|[Solda Fria](http://www.soldafria.com.br/micro-ventilador-80x80x25hs-12v-sem-rolamento-p-4234.html)|5.74| +|6|1|Chave liga/desliga gangorra 20A|[Solda Fria](http://www.soldafria.com.br/chave-gangorra-kcd2-102n-vermelha-c-neon-e-marcacao-p-4534.html)|1.35| +|7|1|Termistor a prova dágua 10K|[Asla](http://asla.com.br/sensor-temperatura-ntc-10k-inox-ip67.html)|26.00| +|8|1|Display LED segmento quádruplo|[Solda Fria](http://www.soldafria.com.br/display-de-led-vermelho-catodo-quadruplo-d4156ksr-056-p-5992.html)|5.41| +|9|1|MOSFET|[Mouser](http://br.mouser.com/Search/ProductDetail.aspx?R=STP36NF06Lvirtualkey51120000virtualkey511-STP36NF06L)|4.2| +|10|4|Resistor 10K 1/4W|[Solda Fria](http://www.soldafria.com.br/resistor-de-10k-carbono-5-14w-mrptlrdr-p-163.html)|0.05| +|11|1|Diodo 1N4007|[Solda Fria](http://www.soldafria.com.br/diodo-1n4007-p-38.html)|0.10| |12|2|Button|[iPrototype](https://iprototype.nl/products/components/buttons-switches/momentary-push-button), [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=14721)|0.55| |13|1|White LED|[iPrototype](https://iprototype.nl/products/components/led-lcd/ledwit), [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=9088)|0.52| |14|1|220 Ohm resistor|[iPrototype](https://iprototype.nl/products/components/resistors/220R), [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=5785)|0.45| From e7739b8fdd4ac7f1dc2b9654d2d3868f3e492f58 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Fri, 13 Feb 2015 16:19:08 -0200 Subject: [PATCH 13/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/1-incubator.md | 84 ++++++++++++++++----------------- 1 file changed, 42 insertions(+), 42 deletions(-) diff --git a/biofactory/class/1-incubator.md b/biofactory/class/1-incubator.md index ef051a39c64bf..adc9f7e6a9033 100644 --- a/biofactory/class/1-incubator.md +++ b/biofactory/class/1-incubator.md @@ -1,71 +1,71 @@ --- layout: page -title: Incubator (Class 1) +title: Incubadora (Class 1) permalink: /biofactory/class/1-incubator/ --- -## Synopsys +## Sinopse -Build your own incubator and start growing microbes under controlled conditions. During the class you will also get to know the fundamentals of microbiology. We’ll use our purple pigment bacteria as a standard, but you can also choose your own favourite biological agent. A crash course Arduino programming and soldering is included. +Construa sua própria encubadora e comece a cultivar seus próprios microorganismos sob condições controladas. Durante as aulas você vai conhecer também os fundamentos da microbiologia. Vamos usar um pigmento roxo padrão para vizualisar as bactérias, mas você também pode optar por seu agente biológico favorito. Um curso intensivo de programação Arduino e soldagem está incluso. -## Schedule +## Agenda -* Introduction -* Microbiology fundamentals & Biological safety instructions -* Arduino programming & soldering -* Incubator design -* Documentating your designs -* Microorganism cultivation & enrichment practical intro - * [Contamination test](/biofactory/class/1-incubator/contamination-test/) - * [Isolation of Yoghurt bacteria](/biofactory/class/1-incubator/yoghurt-bacteria-isolation/) - * [Isolation of Beer yeasts](/biofactory/class/1-incubator/beer-yeasts-isolation/) -* Tour around your lab +* Introdução +* Fundamentos da microbiologia & instruções de segurança biológica +* Programação Arduino & soldagem +* Projeto da incubadora +* Documentação dos projetos +* Introdução prática ao cultivo & enriquecimento de microorganismos + * [Teste de contaminação](/biofactory/class/1-incubator/contamination-test/) + * [Isolamento de bactérias do iogurte](/biofactory/class/1-incubator/yoghurt-bacteria-isolation/) + * [Isolamento de leveduras da cerveja](/biofactory/class/1-incubator/beer-yeasts-isolation/) +* Tour do laboratório -## Incubator Design +## Projeto da incubadora -This week we will build our first Open Hardware lab device: the incubator. Download the design files here: +Essa semana nós vamos construir nosso primeiro dispositivo de laboratório Open Hardware. Baixe os arquivos do projeto aqui: -* [Functional and technical requirements](/biofactory/class/1-incubator/requirements/) -* [SketchUp assembly](/biofactory/class/1/Incubator-Sketchup.skp) +* [Requisitos funcionais e técnicos](/biofactory/class/1-incubator/requirements/) +* [Montagem do SketchUp](/biofactory/class/1/Incubator-Sketchup.skp) * [SVG laser cut files](/biofactory/class/1/Incubator-SVGs.zip) * [Fritzing wiring](/biofactory/class/1/Incubator-Fritzing.fzz) -* [Arduino code](/biofactory/class/1/Incubator-Arduino.txt) +* [Código Arduino](/biofactory/class/1/Incubator-Arduino.txt) -![Incubator](/biofactory/class/1/Incubator.png) +![Incubadora](/biofactory/class/1/Incubator.png) -## Bill of Materials +## Lista de Materiais -In order to take part in this class you will need the following materials: +Para fazer parte dessa aula, você precisará dos seguintes materiais: -* [Incubator materials](/biofactory/class/1-incubator/incubator-materials/) -* [Microbiology practical materials](/biofactory/class/1-incubator/microbiology-materials/) +* [Materiais da incubadora](/biofactory/class/1-incubator/incubator-materials/) +* [Materiais para a prática da microbiologia](/biofactory/class/1-incubator/microbiology-materials/) -## Assignment +## Tarefa -Set up your own documentation site on Github. +Organize sua própria pasta de documentos aqui no Github. -## Additional reading and hacking +## Leituras e hacking adicionais -* Microbiology - * [Microbiology](http://education-portal.com/academy/course/microbiology-course.html) - * [Introduction to Microbiology Culture Techniques](https://www.youtube.com/watch?v=Et1v8EQP10U) YouTube video +* Microbiologia + * [Microbiologia](http://education-portal.com/academy/course/microbiology-course.html) + * [Introdução às Técnicas de Cultivo de Microbiologia](https://www.youtube.com/watch?v=Et1v8EQP10U) YouTube video * Arduino - * [Getting started with Arduino](http://www.makeuseof.com/tag/getting-started-with-arduino-a-beginners-guide/) - * [Ladyada Arduino Tutorials](http://www.ladyada.net/learn/arduino/) - * [Soldering](https://www.youtube.com/watch?v=oqV2xU1fee8) YouTube video -* Incubators + * [Começando com Arduino](http://www.makeuseof.com/tag/getting-started-with-arduino-a-beginners-guide/) + * [Tutoriais Ladyada Arduino ](http://www.ladyada.net/learn/arduino/) + * [Soldagem](https://www.youtube.com/watch?v=oqV2xU1fee8) YouTube video +* Incubadoras * [Climaduino](http://www.instructables.com/id/Introducing-Climaduino-The-Arduino-Based-Thermosta/) - * [Biodesign DIY Incubator](http://biodesign.cc/2013/12/25/diy-incubator/) + * [Bioprojeto DIY Incubadora](http://biodesign.cc/2013/12/25/diy-incubator/) * Github - * [How the heck do I use Github](http://lifehacker.com/5983680/how-the-heck-do-i-use-github) + * [Como raios eu uso o Github?](http://lifehacker.com/5983680/how-the-heck-do-i-use-github) * [Git cheatsheet](http://rogerdudler.github.io/git-guide/) -* Additional Practicals - * [Isolation of Bioluminescent bacteria](/biofactory/class/1-incubator/bioluminescent-bacteria-isolation/) - * [Isolation of Nitrogen-fixating bacteria](/biofactory/class/1-incubator/isolation-of-nitrogen-fixating-bacteria/) - * [Isolation of Sulphur-oxidizing bacteria](/biofactory/class/1-incubator/isolation-of-sulphur-oxidizing-bacteria/) +* Práticas Adicionais + * [Isolamento de bactérias bioluminescentes](/biofactory/class/1-incubator/bioluminescent-bacteria-isolation/) + * [Isolamento de bactérias fixadoras de Nitrogênio](/biofactory/class/1-incubator/isolation-of-nitrogen-fixating-bacteria/) + * [Isolamento de bactérias oxidantes de Enxofre](/biofactory/class/1-incubator/isolation-of-sulphur-oxidizing-bacteria/) -## Feeling lazy? +## Está com preguiça? -Go ahead and buy an incubator: +Vá em frente e compre uma incubadora: * [Lucky Reptile](http://www.amazon.co.uk/Lucky-Reptile-HN-2UK-Nursery-Incubator/dp/B002NFR0HQ) From 15f71b3b81af8c4ed0bc16e9af03feb1d41c7752 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Fri, 13 Feb 2015 16:23:23 -0200 Subject: [PATCH 14/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/1-incubator.md | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/biofactory/class/1-incubator.md b/biofactory/class/1-incubator.md index adc9f7e6a9033..bfedf0b42ab3e 100644 --- a/biofactory/class/1-incubator.md +++ b/biofactory/class/1-incubator.md @@ -6,7 +6,7 @@ permalink: /biofactory/class/1-incubator/ ## Sinopse -Construa sua própria encubadora e comece a cultivar seus próprios microorganismos sob condições controladas. Durante as aulas você vai conhecer também os fundamentos da microbiologia. Vamos usar um pigmento roxo padrão para vizualisar as bactérias, mas você também pode optar por seu agente biológico favorito. Um curso intensivo de programação Arduino e soldagem está incluso. +Construa sua própria incubadora e comece a cultivar seus próprios microorganismos sob condições controladas. Durante as aulas você vai conhecer também os fundamentos da microbiologia. Vamos usar um pigmento roxo padrão para vizualisar as bactérias, mas você também pode optar por seu agente biológico favorito. Um curso intensivo de programação Arduino e soldagem está incluso. ## Agenda @@ -27,8 +27,8 @@ Essa semana nós vamos construir nosso primeiro dispositivo de laboratório Open * [Requisitos funcionais e técnicos](/biofactory/class/1-incubator/requirements/) * [Montagem do SketchUp](/biofactory/class/1/Incubator-Sketchup.skp) -* [SVG laser cut files](/biofactory/class/1/Incubator-SVGs.zip) -* [Fritzing wiring](/biofactory/class/1/Incubator-Fritzing.fzz) +* [Arquivos SVG para corte a laser](/biofactory/class/1/Incubator-SVGs.zip) +* [Diagrama Fritzing](/biofactory/class/1/Incubator-Fritzing.fzz) * [Código Arduino](/biofactory/class/1/Incubator-Arduino.txt) ![Incubadora](/biofactory/class/1/Incubator.png) @@ -48,11 +48,11 @@ Organize sua própria pasta de documentos aqui no Github. * Microbiologia * [Microbiologia](http://education-portal.com/academy/course/microbiology-course.html) - * [Introdução às Técnicas de Cultivo de Microbiologia](https://www.youtube.com/watch?v=Et1v8EQP10U) YouTube video + * [Introdução às Técnicas de Cultivo de Microbiologia](https://www.youtube.com/watch?v=Et1v8EQP10U) * Arduino * [Começando com Arduino](http://www.makeuseof.com/tag/getting-started-with-arduino-a-beginners-guide/) * [Tutoriais Ladyada Arduino ](http://www.ladyada.net/learn/arduino/) - * [Soldagem](https://www.youtube.com/watch?v=oqV2xU1fee8) YouTube video + * [Soldagem](https://www.youtube.com/watch?v=oqV2xU1fee8) * Incubadoras * [Climaduino](http://www.instructables.com/id/Introducing-Climaduino-The-Arduino-Based-Thermosta/) * [Bioprojeto DIY Incubadora](http://biodesign.cc/2013/12/25/diy-incubator/) From 8aa08b86f647ced05c4df6a443c8f65ce090ffc9 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Fri, 13 Feb 2015 16:27:06 -0200 Subject: [PATCH 15/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/10-graduation.md | 18 +++++++++--------- 1 file changed, 9 insertions(+), 9 deletions(-) diff --git a/biofactory/class/10-graduation.md b/biofactory/class/10-graduation.md index 0f7fb83b7b47b..61c95c606a0c8 100644 --- a/biofactory/class/10-graduation.md +++ b/biofactory/class/10-graduation.md @@ -4,20 +4,20 @@ title: Graduation (Class 10) permalink: /biofactory/class/10-graduation/ --- -## Synopsys +## Sinopse -The exact program remains a secret. +O programa exato permanece um mistério. -## Schedule +## Agenda * Demos -* Graduation ceremony -* Party +* Cerimônia de graduação +* Festa -## Assignment +## Tarefa -Relax, we made it! +Relaxe, conseguimos! -## Additional reading and hacking +## Leituras e hacking adicionais -* [Biofactory continued](/biofactory/continued/) +* [Continuação Biofactory](/biofactory/continued/) From b56504a8a59ebd14eeddd64a133952ec817b98d6 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Fri, 13 Feb 2015 17:01:29 -0200 Subject: [PATCH 16/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/2-microscopy.md | 90 +++++++++++++++----------------- 1 file changed, 43 insertions(+), 47 deletions(-) diff --git a/biofactory/class/2-microscopy.md b/biofactory/class/2-microscopy.md index 7c715c482fac3..ec9a1fef5cf50 100644 --- a/biofactory/class/2-microscopy.md +++ b/biofactory/class/2-microscopy.md @@ -4,73 +4,69 @@ title: Microscope (Class 2) permalink: /biofactory/class/2-microscope/ --- -## Synopsys +## Sinopse -We will make our own microscope to take a closer look at how last week’s -cultures have grown. The subject of this class is microbio physiology and -open source publishing. +Nós vamos fazer nosso próprio miscroscópio para olhar mais de perto como as culturas da últimas semanas se desenvolveram. O assunto dessa aula é a fisiologia de microorganismos e publicação open source. +> Dessa vez você pode decidir qual equipamento você gostaria de construir. O microscópio hack de webcam é relativamente simples, comparado com o projeto do microscópio propriamente dito. -> This time you may choose what type of device you like to build. The Microscope Webcam Hack is relatively simple, compared to the full Microscope Design. +## Agenda -## Schedule +* Fisiologia de microorganismos & variedade de nutrientes +* Microscopia & teoria óptica +* Licenciamento e publicação Open Source +* Projeto do microscópio +* Microscopia prática + * [Preparando as lâminas do microscópio ](/biofactory/class/2-microscope/preparing-slides/) + * [Operando um microscópio](/biofactory/class/2-microscope/operating-microscope/) + * [Técnica de coloração Gram](/biofactory/class/2-microscope/gram-staining/) + * [Técnica de coloração Loeffler com Azul de Metileno](/biofactory/class/2-microscope/loeffler-staining/) -* Microbial physiology & Nutrient diversity -* Microscopy & Optics theory -* Open Source licensing & publishing -* Microscope design -* Microscopy practical - * [Preparing microscope slides](/biofactory/class/2-microscope/preparing-slides/) - * [Operating a microscope](/biofactory/class/2-microscope/operating-microscope/) - * [Gram Staining](/biofactory/class/2-microscope/gram-staining/) - * [Loeffler Methylene Blue Staining](/biofactory/class/2-microscope/loeffler-staining/) +## Microscópio Hack com webcam -## Microscope Webcam Hack +Para fazer um microscópio você pode hackear a webcam Hackteria-style -To make a microscope you can hack a webcam Hackteria-style: +* [SketchUp montagem](/biofactory/class/2/Webcam-Microscope-Sketchup.skp) +* [Arquivos SVG de corte a laser](/biofactory/class/2/Webcam-Microscope-SVGs.zip) -* [SketchUp assembly](/biofactory/class/2/Webcam-Microscope-Sketchup.skp) -* [SVG laser cut files](/biofactory/class/2/Webcam-Microscope-SVGs.zip) +![Microscópio de webcam](/biofactory/class/2/Webcam-Microscope.png) -![Webcam Microscope](/biofactory/class/2/Webcam-Microscope.png) +## Projeto de microscópio -## Microscope Design +Construa seu próprio microscópio alternativo. Baixe os arquivos do projeto aqui: -Alternatively build your own microscope. Download the design files here: +* [Requisitos funcionais & técnicos](/biofactory/class/2-microscope/requirements/) +* [SketchUp montagem](/biofactory/class/2/Microscope-Sketchup.skp) +* [Arquivos SVG de corte a laser](/biofactory/class/2/Microscope-SVGs.zip) +* [Diagrama Fritzing](/biofactory/class/2/Microscope-Fritzing.fz) -* [Functional and technical requirements](/biofactory/class/2-microscope/requirements/) -* [SketchUp assembly](/biofactory/class/2/Microscope-Sketchup.skp) -* [SVG laser cut files](/biofactory/class/2/Microscope-SVGs.zip) -* [Fritzing wiring](/biofactory/class/2/Microscope-Fritzing.fz) +![Microscópio](/biofactory/class/2/Microscope.png) -![Microscope](/biofactory/class/2/Microscope.png) +## Lista de materiais -## Bill of Materials +Para fazer parte dessa aula, você precisará dos seguintes materiais: -In order to take part in this class you will need the following materials: +* [Materiais do microscópio de webcam](/biofactory/class/2-microscope/webcam-microscope-materials/) +* [Materiais do microscópio](/biofactory/class/2-microscope/microscope-materials/) +* [Materiais para microscopia prática](/biofactory/class/2-microscope/microscopy-materials/) -* [Webcam Microscope materials](/biofactory/class/2-microscope/webcam-microscope-materials/) -* [Microscope materials](/biofactory/class/2-microscope/microscope-materials/) -* [Microscopy practical materials](/biofactory/class/2-microscope/microscopy-materials/) +## Tarefa -## Assignment +Publique seu video de microscopia juntamente com uma licença Creative Commons na sua página do Github. +## Leitura e hacking adicionais: -Publish your microscopy video along with a Creative Commons license on your Github page. - -## Additional reading and hacking - -* Microscopy - * [Microscopy theory](http://micro.magnet.fsu.edu/primer/anatomy/anatomy.html) +* Microscopia + * [Microscopia teórica](http://micro.magnet.fsu.edu/primer/anatomy/anatomy.html) * [Microbehunter](http://www.microbehunter.com/) - * [Yahoo Microscopy Group](https://groups.yahoo.com/neo/groups/Microscope/info) -* DIY Designs - * [3D printable microscope](http://www.thingiverse.com/thing:77450) + * [Grupo de microscopia no Yahoo](https://groups.yahoo.com/neo/groups/Microscope/info) +* Projetos DIY + * [Microscópio impresso em 3D](http://www.thingiverse.com/thing:77450) * [OneDollar Compound Microscope](http://www.funsci.com/fun3_en/ucomp1/ucomp1.htm) * [Hackteria Microscopy](http://hackteria.org/wiki/index.php/DIY_microscopy) - * [DIYBio Singapore](https://diybiosingapore.wordpress.com/2014/06/22/diy-webcam-microscope-sg-style-2/) - * [Makezine Laser Microscope](http://makezine.com/projects/make-36-boards/laser-projection-microscope/) + * [DIYBio Singapura](https://diybiosingapore.wordpress.com/2014/06/22/diy-webcam-microscope-sg-style-2/) + * [Microscópio a laser Makezine](http://makezine.com/projects/make-36-boards/laser-projection-microscope/) -## Feeling lazy? +## Está com preguiça? -Go ahead and buy a microscope: +Vá em frente e compre um microscópio: -* [LCD video microscope](https://www.conrad.nl/nl/bresser-lcd-microscoop-35-inch-40x-1600x-815889.html) +* [Microscópio de vídeo LCD](https://www.conrad.nl/nl/bresser-lcd-microscoop-35-inch-40x-1600x-815889.html) From e9794b2778563563029465f33e539069241eefca Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Fri, 13 Feb 2015 17:19:45 -0200 Subject: [PATCH 17/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/4-sterile-hood.md | 52 +++++++++++++++--------------- 1 file changed, 26 insertions(+), 26 deletions(-) diff --git a/biofactory/class/4-sterile-hood.md b/biofactory/class/4-sterile-hood.md index 51924cd92fab4..e505addb3e345 100644 --- a/biofactory/class/4-sterile-hood.md +++ b/biofactory/class/4-sterile-hood.md @@ -1,56 +1,56 @@ --- layout: page -title: Sterile Hood (Class 4) +title: Cabine Estéril (Aula 4) permalink: /biofactory/class/4-sterile-hood/ --- > NOTE: these instructions are still under construction. The designs and BoM might be subject to change. -## Synopsys +## Sinopse -In order to make it easier to work sterile with microbes, we will construct our own sterile hood. During class you will learn more about biological safety and how to purify strains. +Para facilitar o trabalho com micoorganismos em ambiente estéril, nós vamos construir nossa própria cabine estéril. Durante a aula você irá aprender mais sobre segurança biológica e como purificar linhagens. -## Schedule +## Agenda -* Biohacking overview -* Biological safety instructions -* Sterile Hood design +* Visão geral sobre Biohacking +* Instruções: Segurança Biológica +* Projeto da cabine estéril -## Sterile Hood Design +## Projeto da cabine estéril -This week we will build the sterile hood. Download the design files here: +Esta semana iremos construir a cabine estéril. Baixe os arquivos do projeto aqui: -* [Functional and technical requirements](/biofactory/class/4-sterile-hood/requirements/) -* [SketchUp assembly](/biofactory/class/4/Sterile-Hood-Sketchup.skp) -* [SVG laser cut files](/biofactory/class/4/Sterile-Hood-SVGs.zip) +* [Requisitos funcionais e técnicos](/biofactory/class/4-sterile-hood/requirements/) +* [Montagem SketchUp](/biofactory/class/4/Sterile-Hood-Sketchup.skp) +* [Arquivos SVG de corte a laser](/biofactory/class/4/Sterile-Hood-SVGs.zip) -![Sterile Hood](/biofactory/class/4/Sterile-Hood.png) +![Cabine Estéril](/biofactory/class/4/Sterile-Hood.png) -No Fritzing wiring this time, just connect the fan to the switch and plug. +Sem driagrama Fritzing desta vez, só conecte o ventilador ao interruptor e ligue. -## Bill of Materials +## Lista de materiais -In order to take part in this class you will need the following materials: +Para fazer parte dessa aula, você precisará dos seguintes materiasi: -* [Sterile Hood materials](/biofactory/class/4-sterile-hood/sterile-hood-materials/) +* [Materiais para Cabine Estéril](/biofactory/class/4-sterile-hood/sterile-hood-materials/) -## Assignment +## Tarefa -1. Search for previous projects within the Biohack community regarding your biofactory and set up a small reference library on your Github Page. -2. Start growing your organism in a liquid culture. +1. Procure por projetos anteriores na comunidade Biohack referentes à sua biofábrica e estabeleça uma pequena biblioteca de referência na sua página do Github. +2. Comece a cultivar seus microorgansimos em uma cultura líquida. -## Additional reading and hacking +## Leituras e hacking adicionais * Biohacking * [Biohacklabs](http://www.biohacklabs.org) * [DIYBio](http://www.diybio.org) * [Hackteria](http://www.hackteria.org) * [Biocoder](http://www.biocoder.com) -* Sterile Hoods - * [Hackteria Sterile Hood](http://hackteria.org/wiki/index.php/DIY_Sterlisation_Hood) +* Cabines Estéreis + * [Cabine Estéril Hackteria](http://hackteria.org/wiki/index.php/DIY_Sterlisation_Hood) -## Feeling lazy? +## Está com preguiça? -Go ahead and buy a sterile hood: +Vá em frente e compre sua própria cabine estéril: -* Buy one on [eBay](http://www.ebay.com/bhp/laminar-flow-hood) +* Compre uma no [eBay](http://www.ebay.com/bhp/laminar-flow-hood) From 4319956a654faea5ad553589d1fd7d13ed1bced0 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Fri, 13 Feb 2015 17:35:28 -0200 Subject: [PATCH 18/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/5-centrifuge.md | 50 ++++++++++++++++---------------- 1 file changed, 25 insertions(+), 25 deletions(-) diff --git a/biofactory/class/5-centrifuge.md b/biofactory/class/5-centrifuge.md index aba365d116761..38a198d0b0111 100644 --- a/biofactory/class/5-centrifuge.md +++ b/biofactory/class/5-centrifuge.md @@ -6,52 +6,52 @@ permalink: /biofactory/class/5-centrifuge/ > NOTE: these instructions are still under construction. The designs and BoM might be subject to change. -## Synopsys +## Sinopse -We’ll try to isolate our first biomaterial. For that we need centrifugal force to take your samples for a spin. In class you will learn more about analog electronics and physical separation techniques. +Vamos tentar isolar nosso primeiro material biológico. Para isso nós precisamos da força centrífuga para girar suas amostras. Nesta aula você vai aprender mais sobre eletronica analógica e técnicas de separação física. -## Schedule +## Agenda -* Electronics -* Fritzing documentation +* Electronica +* Documentação Fritzing * OpenSCAD -* Centrifugation -* Centrifuge design +* Centrifugação +* Projeto da centrífuga -## Centrifuge Design +## Projeto da centrífuga -This week we will build the centrifuge. Download the design files here: +Essa semana iremos construir a centrífuga. Baixe os arquivos do projeto aqui: -* [Functional and technical requirements](/biofactory/class/5-centrifuge/requirements/) -* [SketchUp assembly](/biofactory/class/5/Centrifuge-Sketchup.skp) -* [SVG laser cut files](/biofactory/class/5/Centrifuge-SVGs.zip) -* [Fritzing wiring](/biofactory/class/5/Centrifuge-Fritzing.fz) +* [Requisitos técnicos e funcionais](/biofactory/class/5-centrifuge/requirements/) +* [SketchUp montagem](/biofactory/class/5/Centrifuge-Sketchup.skp) +* [Arquivos SVG de corte a laser](/biofactory/class/5/Centrifuge-SVGs.zip) +* [Diagrama Fritzing](/biofactory/class/5/Centrifuge-Fritzing.fz) -![Sterile Hood](/biofactory/class/5/Centrifuge.png) +![Cabine estéril](/biofactory/class/5/Centrifuge.png) -## Bill of Materials +## Lista de materiais -In order to take part in this class you will need the following materials: +Para fazer parte desta aula, você vai precisar dos seguintes materiais: -* [Centrifuge materials](/biofactory/class/5-centrifuge/centrifuge-materials/) +* [Materiais da centrífuga](/biofactory/class/5-centrifuge/centrifuge-materials/) -## Assignment +## Tarefa -Start sketching your bioreactor controller in Fritzing. +Comece desenhando seu controlador de biorreator no Fritzing. -## Additional reading and hacking +## Leitura e hacking adicionais: -* Electronics - * [Electronics for Dummies](http://www.amazon.com/Electronics-For-Dummies-Cathleen-Shamieh/dp/0470286970) +* Electronica + * [Eletronica para leigos](http://www.amazon.com/Electronics-For-Dummies-Cathleen-Shamieh/dp/0470286970) * Fritzing - * [Fritzing Tutorials](http://fritzing.org/learning/tutorials) + * [Tutorial Fritzing](http://fritzing.org/learning/tutorials) * Centrifugation * [Forbidden F Word](http://www.physicsclassroom.com/class/circles/Lesson-1/The-Forbidden-F-Word) * Centrifuge * [RWXBioFuge](https://github.com/PieterVanBoheemen/RWXBioFuge) -## Feeling lazy? +## Está com preguiça? -Go ahead and buy a table top centrifuge: +Vá em frente e compre sua própria centrífuga de bancada: * [Eppendorf 5424](http://www.eppendorf.com/int/index.php?sitemap=2.1&action=products&contentid=1&catalognode=22420) From cf18993a81ee209f3e4d6ee5df690649785adf78 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Fri, 13 Feb 2015 17:47:43 -0200 Subject: [PATCH 19/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/6-magnetic-stirrer.md | 50 +++++++++++++------------- 1 file changed, 25 insertions(+), 25 deletions(-) diff --git a/biofactory/class/6-magnetic-stirrer.md b/biofactory/class/6-magnetic-stirrer.md index 0f0cddd0b21bd..340aa73807496 100644 --- a/biofactory/class/6-magnetic-stirrer.md +++ b/biofactory/class/6-magnetic-stirrer.md @@ -1,51 +1,51 @@ --- layout: page -title: Magnetic Stirrer (Class 6) +title: Agitador Magnético (Aula 6) permalink: /biofactory/class/6-magnetic-stirrer/ --- > NOTE: these instructions are still under construction. The designs and BoM might be subject to change. -## Synopsys +## Sinopse -In order to scale up our BioFactory, we need to stir things up. Production is often limited by mass transport, so the stirrer will fix that. We’ll explore the basics of bioreactor design too. +Para ampliar nossa Biofábrica, nós precisamos agitar um pouco as coisas. A produção é geralmente limitada pelo transporte de massa, então o agitador irá corrigir isso. Vamos explorar o básico do projeto do biorreator também. -## Schedule +## Agenda -* Physical Transport Phenonoma -* Bioreactor Design -* Magnetic Stirrer Design +* Fenômenos de Transporte Físico +* Projeto do Biorreator +* Projeto do Agitador Magnético -## Stirrer Design +## Projeto do Agitador -This week we will build the stirrer. Download the design files here: +Esta semana vamos construir o agitador. Baixe os aqrquivos do projeto aqui: -* [Functional and technical requirements](/biofactory/class/6-stirrer/requirements/) -* [SketchUp assembly](/biofactory/class/6/Magnetic-Stirrer-Sketchup.skp) -* [SVG laser cut files](/biofactory/class/6/Magnetic-Stirrer-SVGs.zip) -* [Fritzing wiring](/biofactory/class/6/Magnetic-Stirrer-Fritzing.fz) +* [Requisitos Técnicos e Funcionais](/biofactory/class/6-stirrer/requirements/) +* [Montagem do SketchUp](/biofactory/class/6/Magnetic-Stirrer-Sketchup.skp) +* [Arquivos SVG de corte a laser](/biofactory/class/6/Magnetic-Stirrer-SVGs.zip) +* [Diagrama Fritzing](/biofactory/class/6/Magnetic-Stirrer-Fritzing.fz) -![Magnetic Strirrer](/biofactory/class/6/Magnetic-Stirrer.png) +![Agitador Magnetico](/biofactory/class/6/Magnetic-Stirrer.png) -## Bill of Materials +## Lista de Materiais -In order to take part in this class you will need the following materials: +Para tomar parte nesta aula você irá precisar dos seguintes materiais: -* [Magnetic Stirrer materials](/biofactory/class/6-magnetic-stirrer/stirrer-materials/) +* [Materiais Agitador Magnetico](/biofactory/class/6-magnetic-stirrer/stirrer-materials/) -## Assignment +## Tarefa -Design your bioreactor dimensions and fluxes. +Projete as dimensões e fluxos do seu biorreator. -## Additional reading and hacking +## Leituras e hacking adicionais -* Physical Transport Phenonoma -* Bioreactor Design -* Magnetic Stirrer +* Fenômenos de Transporte Físico +* Projeto do biorreator +* Agitador Magnético * [TeklaLabs](http://www.teklalabs.org/magnetic-stirrer/) -## Feeling lazy? +## Está com preguiça? -Go ahead and buy a stirrer: +Vá em frente e compre um agitador: * [VWR](https://us.vwr.com/store/catalog/category.jsp?id=597830) From f06cc017339feb37e8dfc4e1a6b62e049ff1c2bd Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Fri, 13 Feb 2015 18:15:24 -0200 Subject: [PATCH 20/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/7-spectrometer.md | 68 +++++++++++++++--------------- 1 file changed, 34 insertions(+), 34 deletions(-) diff --git a/biofactory/class/7-spectrometer.md b/biofactory/class/7-spectrometer.md index 54f987cdf1a78..833bbb829289b 100644 --- a/biofactory/class/7-spectrometer.md +++ b/biofactory/class/7-spectrometer.md @@ -6,62 +6,62 @@ permalink: /biofactory/class/7-spectrometer/ > NOTE: these instructions are still under construction. The designs and BoM might be subject to change. -## Synopsys +## Sinopse -Spectroscopy is an essential tool for monitoring the growth and production in your bioreactor. We will make our own light source and detector setup. The class is about other analytical techniques too. +Espectroscopia é uma ferramenta essencial para a monitorizar o cultivo e produção no seu bioreator. Nós iremos fazer nossa própria fonte de luz e configuração do detector. A aula é também sobre outras técnicas analíticas. -## Schedule +## Agenda -* Acids & Bases -* Spectroscopy & Spectrometry -* Analytical techniques -* Spectrometer Design -* Spectrometry Practical - * Iron Calibration Curve +* Ácidos e bases +* Espectroscopia & Espectrometria +* Técnicas analíticas +* Projeto do espectrômetro +* Prática Espectrométrica + * Curva de calibração de ferro -## Spectrometer Design +## Projeto do espectrômetro -This week we will build the spectrometer. You may choose which design. Download the design files here: +Essa semana iremos construir o espectrômetro. Você pode escolher qual projeto. Baixe os arquivos do projeto aqui: -* [Functional and technical requirements](/biofactory/class/7-spectrometer/requirements/) -* Webcam design - * [SketchUp assembly](/biofactory/class/7/Webcam-Spectrometer-Sketchup.skp) - * [SVG laser cut files](/biofactory/class/7/Webcam-Spectrometer-SVGs.zip) +* [Requisitos técnicos e funcionais](/biofactory/class/7-spectrometer/requirements/) +* Projeto da Webcam + * [SketchUp montagem](/biofactory/class/7/Webcam-Spectrometer-Sketchup.skp) + * [Arquivos SVG para corte a laser](/biofactory/class/7/Webcam-Spectrometer-SVGs.zip) -![Webcam Spectrometer](/biofactory/class/7/Webcam-Spectrometer.png) +![Espectrômetro de webcam](/biofactory/class/7/Webcam-Spectrometer.png) -* Photodiode array design - * [SketchUp assembly](/biofactory/class/7/CCD-Array-Spectrometer-Sketchup.skp) - * [SVG laser cut files](/biofactory/class/7/CCD-Array-Spectrometer-SVGs.zip) - * [Fritzing wiring](/biofactory/class/7/Spectrometer-Fritzing.fzz) +* Projeto de arranjo de fotodiodo + * [SketchUp montagem](/biofactory/class/7/CCD-Array-Spectrometer-Sketchup.skp) + * [Arquivos SVG de corte a laser](/biofactory/class/7/CCD-Array-Spectrometer-SVGs.zip) + * [Diagrama Fritzing](/biofactory/class/7/Spectrometer-Fritzing.fzz) -![CCD Array Spectrometer](/biofactory/class/7/CCD-Array-Spectrometer.png) +![Arranjo CCD do Espectrômetro](/biofactory/class/7/CCD-Array-Spectrometer.png) -## Bill of Materials +## Lista de materiais -In order to take part in this class you will need the following materials: +Para participar desta aula, você irá precisar dos seguintes materiais? -* [Spectrometer materials](/biofactory/class/7-spectrometer/spectrometer-materials/) -* Spectrometry materials +* [Materiais do Espectrômetro](/biofactory/class/7-spectrometer/spectrometer-materials/) +* Materiais espectromia -## Assignment +## Tarefa -Hack the spectrometer into a flow spectrometer. +Transforme o espectrômetro convencial em um espectrômetro de fluxo. -## Additional reading and hacking +## Leitura e hacking adicionais: -* Spectroscopy +* Espectroscopia * [PublicLab](http://publiclab.org/wiki/spectrometer) -* DIY Spectrometers +* DIY Espectrômetros * [Really Basic - Instructable](http://www.instructables.com/id/DIY-Spectroscope/) * [Spectruino](http://myspectral.com/) * [CCD array](http://publiclab.org/notes/bhickman/10-12-2013/ccd-diodearray-spectrometer) * [TSL1402R](http://playground.arduino.cc/Main/TSL1402R) -* DIY absorbance meters +* DIY medidor de absorvência * [Open Source Colorimeter](http://www.appropedia.org/Open-source_colorimeter) -## Feeling lazy? +## Está com preguiça? -Go ahead and buy a spectrometer: +Vá em frente e compre seu espectrômetro: -* Second hand photospectrometers at [LabX](http://www.labx.com/spectrophotometer-uv-vis-fluor) +* Fotoespectrômetros de segunda mão: [LabX](http://www.labx.com/spectrophotometer-uv-vis-fluor) From 4d9f49a85ef2c4d09fddc0c29e3cd536a4b42d0a Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Fri, 13 Feb 2015 18:34:53 -0200 Subject: [PATCH 21/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/8-pumps.md | 66 ++++++++++++++++++------------------- 1 file changed, 33 insertions(+), 33 deletions(-) diff --git a/biofactory/class/8-pumps.md b/biofactory/class/8-pumps.md index 037005a7aecb4..74871d52b10e8 100644 --- a/biofactory/class/8-pumps.md +++ b/biofactory/class/8-pumps.md @@ -1,64 +1,64 @@ --- layout: page -title: Syringe & Peristaltic Pump (Class 8) +title: Bomba de seringa e peristáltica (Aula 8) permalink: /biofactory/class/8-pumps/ --- > NOTE: these instructions are still under construction. The designs and BoM might be subject to change. -## Synopsys +## Sinopse -The microbes in your bioreactor need fresh food. Let’s get rid of the manual labour and connect syringe & peristaltic pumps. You will also learn more about feed stocks and other substrates. We’ll go a bit deeper into metabolic networks too. +Os microorganismos no seu biorreator precisam de comida fresca. Vamos nos livrar do trabalho manual e conectar as bombas de seringa e peristáltica. Você também aprenderá mais sobre estoques de alimentação e outros substratos. Vamos um pouquinho mais a fundo em redes metabólicas também. -## Schedule +## Agenda -* Metabolic networks & flux analysis -* Mechinical drive materials -* Syringe pump design -* Peristaltic pump design +* Redes metabólicas e análise de fluxo +* Materiais de acionamento mecânico +* Projeto da bomba de seringa +* Projeto da bomba peristáltica -## Pump Designs +## Projetos das bombas -This week we will two type of pumps. Download the design files here: +Esta semana vamos construir dois tipos de bomba. Baixe os arquivos dos projetos aqui: -* [Functional and technical requirements](/biofactory/class/8-pumps/requirements/) -* Syringe pump design - * [SketchUp assembly](/biofactory/class/8/Syringe-Pump-Sketchup.skp) - * [SVG laser cut files](/biofactory/class/8/Syringe-Pump-SVGs.zip) - * [Fritzing wiring] - * [Arduino Code] +* [Requisitos técnicos e funcionais](/biofactory/class/8-pumps/requirements/) +* Projeto Bomba de seringa + * [Montagem do SketchUp](/biofactory/class/8/Syringe-Pump-Sketchup.skp) + * [Arquivos SVG para corte a laser](/biofactory/class/8/Syringe-Pump-SVGs.zip) + * [Diagrama Fritzing] + * [Código Arduino] -![Syringe Pump](/biofactory/class/8/Syringe-Pump.png) +![Bomba de Seringa](/biofactory/class/8/Syringe-Pump.png) -* Peristaltic pump design - * SketchUp assembly - * SVG laser cut files - * [Fritzing wiring] - * [Arduino Code] +* Projeto da bomba peristáltica + * Montagem do SketchUp + * Arquivos SVG para corte a laser + * [Diagrama Fritzing] + * [Código Arduino] -## Bill of Materials +## Lista de Materiais -In order to take part in this class you will need the following materials: +Para tomar parte nesta aula você irá precisar dos seguintes materiais: -* [Syringe Pump materials](/biofactory/class/8-pumps/syringe-pump-materials/) -* [Peristaltic Pump materials](/biofactory/class/8-pumps/peristaltic-pump-materials/) +* [Materiais para bomba de seringa](/biofactory/class/8-pumps/syringe-pump-materials/) +* [Materiais para bomba peristáltica](/biofactory/class/8-pumps/peristaltic-pump-materials/) -## Assignment +## Tarefa -Document one of your devices in as an instructable. +Documente um dos seus equipamentos como um guia. -## Additional reading and hacking +## Leitura e hacking adicionais * Metabolomics * [Wikipedia Flux Analysis](http://en.wikipedia.org/wiki/Flux_balance_analysis -* DIY Syringe pumps +* DIY Bombas de Seringa * [OpenPump](https://www.wevolver.com/gerrit.niezen/openpump---an-open-source-hardware-syringe-pump/openpump) -* DIY Peristaltic pumps +* DIY Bombas peristálticas * [Instrutable Peristaltic Pump](http://www.instructables.com/id/Inexpensive-easy-to-build-peristaltic-pump/) -## Feeling lazy? +## Está com preguiça? -Go ahead and buy some pumps: +Vá em frente e compre algumas bombas: * [Syringepump.com](http://www.syringepump.com/index.php) * [LabX Peristaltic Pump](http://www.labx.com/pumps-peristaltic) From 7a372462f0f339402f96d5684d25917623f3e272 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Fri, 13 Feb 2015 18:58:04 -0200 Subject: [PATCH 22/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/9-bioreactor-controller.md | 52 ++++++++++----------- 1 file changed, 26 insertions(+), 26 deletions(-) diff --git a/biofactory/class/9-bioreactor-controller.md b/biofactory/class/9-bioreactor-controller.md index de85b0af0e6c0..37a02844c9810 100644 --- a/biofactory/class/9-bioreactor-controller.md +++ b/biofactory/class/9-bioreactor-controller.md @@ -6,49 +6,49 @@ permalink: /biofactory/class/9-reactor-controller/ > NOTE: these instructions are still under construction. The designs and BoM might be subject to change. -## Synopsys +## Sinopse -Now that we have constructed pumps, stirrers and a spectrometer we can start connecting all the dots into one big bioreactor. The controller will be able to set the environmental parameters, input and analyse the effluent of your reactor. +Agora que nós construimos as bombas, agitadores e um espectrômetro nós podemos começar conectando todos os pontos em um grande biorreator. O controlador vai ser capaz de definir os parametros ambientais, adentrar e analisar o efluente de seu reator. -## Schedule +## Agenda -* Reactor assembly -* Inoculation and sampling -* Controller Design +* Montagem do reator +* Inoculação e amostragem +* Projeto do controlador -## Reactor Controller Design +## Projeto do reator controlador -This week is all about Arduino code. We need to tie everything together into one mean machine. +Essa semana irá ser toda sobre o código Arduino. Precisamos amarrar tudo junto em uma máquina muito louca. -* [Arduino code](/biofactory/class/19/reactor-controller-arduino-code.txt) +* [Código Arduino](/biofactory/class/19/reactor-controller-arduino-code.txt) -## Bill of Materials +## Lista de materiais -In order to take part in this class you will need the following materials: +Para fazer parte desta aula, você precisará dos seguintes materiais: -* [Reactor Controller materials](/biofactory/class/9-reactor-controller/controller-materials/) +* [Materiais de controle do reator](/biofactory/class/9-reactor-controller/controller-materials/) -## Assignment +## Tarefa -Document your entire bioreactor, anwering the following questions: +Documente seu biorreator por inteiro, respondendo as seguintes perguntas: -* What does the reactor do? What product has been made? -* From what designs is it derived? -* How much does it cost? -* Which parts have been custom made, by what machine? -* What are all the components and how much do they cost? -* How is it assembled? -* What can be improved? +* O que o reator faz? Qual produto foi feito? +* De que projetos ele é derivado? +* Quanto custa? +* Que partes foram customizadas, e de que máquinas? +* Quais são todos os componentes e quanto eles custam? +* Como é montado? +* O que pode ser melhorado? -## Additional reading and hacking +## Leitura e hacking adicionais: -* Reactor Design +* Projeto do reator * -* Controllers +* Controladores * -## Feeling lazy? +## Está com preguiça? -Go ahead and buy a reactor controller: +Vá em frente, e compre seu próprio controlador de biorreator: * [Applikon](https://www.applikon-biotechnology.us/products/bioreactors) From 8de85dee7c69a459add6ac63df41f88ac8af3592 Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Wed, 18 Feb 2015 18:07:20 -0200 Subject: [PATCH 23/40] =?UTF-8?q?tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/3-design.md | 36 ++++++++++++++++++------------------ 1 file changed, 18 insertions(+), 18 deletions(-) diff --git a/biofactory/class/3-design.md b/biofactory/class/3-design.md index 467e8063cf8a6..b6f94c990482f 100644 --- a/biofactory/class/3-design.md +++ b/biofactory/class/3-design.md @@ -1,41 +1,41 @@ --- layout: page -title: 2D & 3D Design (Class 3) +title: 2D & 3D Design (Aula 3) permalink: /biofactory/class/3-design/ --- > NOTE: these instructions are still under construction. The designs and BoM might be subject to change. -## Synopsys +## Sinopse -This class is essential. You will learn how to design objects in 2D and 3D and transform them into real objects with a lasercutter or 3D printer. As a demo we will make basic lab utilities, such as tube racks. +Esta aula é essencial. Você aprenderá como projetar objetos em 2D e 3D e transformá-los em objetos reais com a cortadora a laser ou impressora 3D. Como demonstração faremos utensílios básicos de laboratório, tais como suportes para tubos de ensaio. -## Schedule +## Agenda -* Digital Fabrication & Materials -* Construction +* Fabricação Digital e Materiais +* Construção * SketchUp * OpenSCAD -* Practicals - * 3D Printing - * Lasercutting +* Prática + * Impressão 3D + * Corte a laser -## Example files +## Exemplos de arquivos * SketchUp - * Wood joints + * Junções de madeira * OpenSCAD - * [Centrifuge rotor](/biofactory/class/3/Rotor.scad) + * [Rotor da centrífuga](/biofactory/class/3/Rotor.scad) -## Assignment +## Tarefa -Design a personal laboratory tool +Projete um utensílio de laboratório pessoal -## Additional reading and hacking +## Leitura e hacking adicionais -* 3D Design +* Projeto 3D * [OpenSCAD](http://blog.openpump.org/how-to-design-a-3d-printed-enclosure-for-your-product/) -* 2D Design +* Projeto 2D * [CNC panel joinery notebook](http://makezine.com/2012/04/13/cnc-panel-joinery-notebook/) -* Materials +* Materiais * [Fablab Amsterdam Suppliers](http://fablab.waag.org/views/suppliers) From de65d8807546befb930e6d6171fceb7bddfdd461 Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Wed, 18 Feb 2015 19:02:43 -0200 Subject: [PATCH 24/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- ...isolation of nitrogen-fixating bacteria.md | 46 +++++++++---------- 1 file changed, 23 insertions(+), 23 deletions(-) diff --git a/biofactory/class/1/isolation of nitrogen-fixating bacteria.md b/biofactory/class/1/isolation of nitrogen-fixating bacteria.md index 98dc5aa6ec9b9..c86c8c0db5c02 100644 --- a/biofactory/class/1/isolation of nitrogen-fixating bacteria.md +++ b/biofactory/class/1/isolation of nitrogen-fixating bacteria.md @@ -4,33 +4,33 @@ title: Isolation of Nitrogen-fixating bacteria permalink: /biofactory/class/1-incubator/isolation-of-nitrogen-fixating-bacteria/ --- -## Goal +## Objetivo -N2 is a gas that ubiquitously available in the atmosphere. Some special bacteria are capable of fixating it and thereby make it available for other life forms. In this experiment we will isolate them from soil. +N2 (Nitrogênio) é um gás onipresente disponível na atmosfera. Algumas bactérias especiais são capazes de fixá-lo e assim torná-lo disponível para outras formas de vida. Neste experimento iremos isolá-las do solo. -## Materials +## Materiais -* 100 mL of [Azotobacter medium](http://127.0.0.1:4000/biofactory/annex/cultivation-media/azotobacter-medium/) +* 100 mL de [meio Azotobacter](http://127.0.0.1:4000/biofactory/annex/cultivation-media/azotobacter-medium/) * 1 [Azotobacter Agar](http://127.0.0.1:4000/biofactory/annex/cultivation-media/azotobacter-agar/) plate -* 3 100 mL erlenmeyer flasks -* Soil -* Nitrogen test strips - -## Method - -1. Prepare 2 Erlenmeyer flasks by transfering 25 mL into each. -2. Add some soil to one of the Erlenmeyer flasks. -3. Save the other Erlenmeyer as negative sterile control. -4. Clearly label the flasks: name, date & content. -5. Incubate the flasks at 30° C. -6. Inspect the flasks every day or two. -7. As soon as (most likely after one week) a small film can be observed on the surface of the medium, transfer a small amount into a fresh Erlenmeyer flask. -8. Incubate this flask again at 30° C. -9. Look at the biofilm under the microscope. -10. Take a sample from the flasks and check the NH+ concentration with the test strip. -11. As soon as growth can be observed in the 3rd flask, streak a Azotobacter agar plate and incubate at 30° C. Lable the plate: name, date & content. -12. Look at a sample under the microscope. -13. Check after 24h. +* 3 frascos erlenmeyer 100 mL +* Solo +* Tiras para teste de Nitrogênio + +## Método + +1. Prepare 2 frascos Erlenmeyer transferindo 25 mL em cada. +2. Adicione um pouco de solo a um dos frascos Erlenmeyer. +3. Guarde o outro Erlenmeyer como controle estéril negativo. +4. Rotule os frascos de forma clara: nome, data & conteúdo. +5. Incube os frascos a 30 graus Celsius. +6. Inspecione os frascos a cada um dia ou dois. +7. Assim que (mais provavelmente após uma semana) uma pequena membrana pode ser observada na superfície do meio de cultura, transfira uma pequena quantidade para um frasco Erlenmeyer fresco. +8. Incube o frasco novamente a 30 graus Celsius. +9. Observe o biofilme sob o microscópio. +10. Pegue uma amostra dos frascos e cheque a concentração de NH+ com as tiras de teste. +11. Assim que o crescimento possa ser observado no terceiro frasco, estrie uma placa de agar Azotobacter e incube-a a 30 graus Celsius. Rotule as placas: nome, data & conteúdo. +12. Observe as amostras sob o microscópio. +13. Cheque após 24 horas. Back to [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) From 6623dd496bf6fdc86eda8898a3c0fe3a12aa674d Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Wed, 18 Feb 2015 19:08:16 -0200 Subject: [PATCH 25/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/1/isolation of yoghurt bacteria.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/biofactory/class/1/isolation of yoghurt bacteria.md b/biofactory/class/1/isolation of yoghurt bacteria.md index bbc46199e42f7..a18708ab071ac 100644 --- a/biofactory/class/1/isolation of yoghurt bacteria.md +++ b/biofactory/class/1/isolation of yoghurt bacteria.md @@ -1,6 +1,6 @@ --- layout: page -title: Isolation of Yoghurt bacteria +title: Isolamento de Bactérias do Iogurte permalink: /biofactory/class/1-incubator/yoghurt-bacteria-isolation/ --- From 24211e26ff43187fbff733468d4672d96d2f62b5 Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Wed, 18 Feb 2015 19:29:29 -0200 Subject: [PATCH 26/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/1/microbiology-materials.md | 34 ++++++++++---------- 1 file changed, 17 insertions(+), 17 deletions(-) diff --git a/biofactory/class/1/microbiology-materials.md b/biofactory/class/1/microbiology-materials.md index 4f05bbd80b433..2cd9290b22c1d 100644 --- a/biofactory/class/1/microbiology-materials.md +++ b/biofactory/class/1/microbiology-materials.md @@ -1,14 +1,14 @@ --- layout: page -title: Microbiology Practical Materials +title: Materiais para Práticas Microbiológicas permalink: /biofactory/class/1-incubator/microbiology-materials/ --- -When following the Biohack Academy, all these materials are provided for by Waag Society. This list is just meant for future reference. +Quando estiver dentro da Biohack Academy, todos estes materiais são fornecidos pela Waag Society. Esta lista é sugerida para referências futuras. -## Compounds +## Componentes -|#|Compound|Prof Supplier|Alternative| +|#|Componentes|Prof Supplier|Alternativas| |-:|:----|:---------|-------:|:---| |1|Ethanol (70%)||[DeWeegschaal](http://www.deweegschaal.nl/chempropack/alcohol-70-ethanol-10-isopropanol-250ml) [Drogist](https://www.drogist.nl/tendo-alcohol-70-gedenatureerd-120-ml.htm?utm_source=beslist.nl&utm_medium=feed&utm_campaign=vergelijken&utm_content=default3) [Kruidvat](https://www.kruidvat.nl/kruidvat-70-alcohol-ketonatus/p/2639904)| |2|Bacteriological Agar|[SigmaAldrich](http://www.sigmaaldrich.com/catalog/product/sigma/a6686?lang=en®ion=NL)|Agar Agar, [Guar Gum](http://www.amazon.com/NOW-Foods-Guar-Powder-Ounces/dp/B0015BWIZS)[Reference](http://www.ncbi.nlm.nih.gov/pubmed/16162142)| @@ -38,19 +38,19 @@ When following the Biohack Academy, all these materials are provided for by Waag |26|Safranin|[SigmaAldrich](http://www.sigmaaldrich.com/catalog/product/sigma/84120?lang=en®ion=NL)|Gram staining kit [Amazon](http://www.amazon.com/Grams-Safranin-Stain-250-mL/dp/B0017U7W6W)| |27|Thiosulfate|[SigmaAldrich](http://www.sigmaaldrich.com/catalog/product/sial/217263?lang=en®ion=NL)|online lab stores [Amazon](http://www.amazon.com/Hanna-Instrument-HI70439-Thiosulfate-Solution/dp/B0085XM8HC/ref=sr_1_7?s=industrial&ie=UTF8&qid=1419354374&sr=1-7&keywords=thiosulfate)| -## Materials +## Materiais -* Glycerol - Sold as "Glycerine" in drug stores -* Nitrogen test strips - Sold in aquarium stores -* pH test strips - Sold in aquarium stores -* 9 cm Petridishes - Home brewing store -* 100 mL Erlenmeyer flasks - Home brewing store or [lab glass store](http://www.laboratoriumglas.eu/index.php?item=&action=page&group_id=10000006&lang=NL) -* 500 mL GL Bottles - [Lab glass store](http://www.laboratoriumglas.eu/index.php?item=labfles-500-ml-met-gl-45&action=article&group_id=56&aid=301&lang=NL) -* 15mL Test tubes - Home brewing store -* 100 mL Beaker glasses - Home brewing store or [Lab glass store](http://www.laboratoriumglas.eu) -* Glass spatula - Also sometimes refered to as "Drigalski Spatula" [VWR](https://nl.vwr.com/store/catalog/product.jsp?catalog_number=231-2183) -* Inoculation wire - just take a piece of metal wire -* Aquarium pump, tube and diffuser - aquarium store obviously +* Glicerol - Vendido como "Glicerina" em farmácias +* Tiras para teste de Nitrogênio - Vendidas em lojas de aquarismo +* Tiras para teste de pH - Vendidas em lojas de aquarismo +* Placas de Petri de 9 cm - Loja de artigos para cervejaria +* Frascos 100 mL Erlenmeyer - Loja de artigos para cervejaria ou [lab glass store](http://www.laboratoriumglas.eu/index.php?item=&action=page&group_id=10000006&lang=NL) +* Garrafas GL de 500 mL - [Lab glass store](http://www.laboratoriumglas.eu/index.php?item=labfles-500-ml-met-gl-45&action=article&group_id=56&aid=301&lang=NL) +* Tubos de ensaio de 15mL - Loja de artigos para cervejaria +* Provetas 100 mL - Loja de artigos para cervejaria [Lab glass store](http://www.laboratoriumglas.eu) +* Espatula de Vidro - Também citada como "Espátula de Drigalski" [VWR](https://nl.vwr.com/store/catalog/product.jsp?catalog_number=231-2183) +* Alça de Drigalski - ou simplesmente use um pedaço de arame +* Bomba de aquário, tubo e difusor - lojas de aquarismo -Back to [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) +De volta à [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) From 747c283e806e564c0fa71a53020533d755709171 Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Wed, 18 Feb 2015 19:52:43 -0200 Subject: [PATCH 27/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/1/requirements.md | 49 +++++++++++++++--------------- 1 file changed, 24 insertions(+), 25 deletions(-) diff --git a/biofactory/class/1/requirements.md b/biofactory/class/1/requirements.md index 289fe3e4382a3..40a6cbc3a6c6a 100644 --- a/biofactory/class/1/requirements.md +++ b/biofactory/class/1/requirements.md @@ -1,39 +1,38 @@ --- layout: page -title: Incubator requirements +title: Requisitos Incubadora permalink: /biofactory/class/1-incubator/requirements/ --- -## Functional requirements +## Requisitos Funcionais -**Must have** +**Deve-se ter** -1. Heat isolated enclosed cabinet, with see-through window -2. Heat source -3. Temperature controller -4. Temperature indicator -5. User interface to set temperature +1. Cabine fechada de isolamento térmico, com janela +2. Fonte de calor +3. Controlador de temperatura +4. Indicador +5. Interface com o usuário para definir temperatura -**Nice to have** +**É bom ter ** -1. Open door detector -2. Temperature alarm +1. Detector de abertura da porta +2. Alarme de temperatura -## Technical requirements +## Requisitos técnicos +**Deve-se ter** -**Must have** +1. Caixa com isolamento de poliestireno com porta transparente + * Medidas externas 35 (w) x 45 (d) x 50 (h) cm + * Medidas internas 25 (w) x 30 (d) x 40 (h) cm +2. Resistor de calor, aletas de refrigeração e ventilador controlado por um relé +3. Arduino UNO microprocessador +4. Sensor de temperatura e mostrador +5. 2 botões de apertar para aumentar/diminuir a temperatura definida -1. Box with expanded polystyrene isolation with a transparent door - * Outer measures 35 (w) x 45 (d) x 50 (h) cm - * Inner measures 25 (w) x 30 (d) x 40 (h) cm -2. Heat resistor, cooling ribs and fan controlled by a relay -3. Arduino UNO microprocessor -4. Temperature sensor and display -5. 2 push buttons for increasing / decrease the set temperature +**É bom ter** -**Nice to have** +1. Interruptor de alavanca +2. Auto-falante -1. Lever switch -2. Speaker - -Back to [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) +De volta à [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/) From 7bd6eb3827fbeaed4ed8781d079a72be54076684 Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Wed, 18 Feb 2015 20:21:49 -0200 Subject: [PATCH 28/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/2/preparing-slides.md | 60 +++++++++++++------------- 1 file changed, 30 insertions(+), 30 deletions(-) diff --git a/biofactory/class/2/preparing-slides.md b/biofactory/class/2/preparing-slides.md index 8d0da3f3d8a81..65f909b9a7b72 100644 --- a/biofactory/class/2/preparing-slides.md +++ b/biofactory/class/2/preparing-slides.md @@ -1,43 +1,43 @@ --- layout: page -title: Preparing Microscope Slides +title: Preparando lâminas de microscopia permalink: /biofactory/class/2-microscope/preparing-slides/ --- -## Goal +## Objetivo -In order to take a close look at a biological sample, you will need to prepare a microscope slide. +Para dar uma olhada mais de perto em amostras biológicas, você precisará preparar uma lâmina de microscópio. -## Materials +## Materiais -* 1 Microscope object slide -* 1 Microscope cover slide -* 1 Gasburner -* 1 Glass pasteur pipette or micropipette with sterile tip -* 1 Paper towel -* a droplet of demi water -* 1 inoculation loop +* 1 lâmina de microscópio +* 1 lamínula de microscópio +* 1 Bico de Bunsen - ou uma chama qualquer a gás +* 1 pipeta pasteur de vidro ou micropipeta com ponteira estéril +* 1 toalha de papel +* 1 gotícula de demi água +* 1 alça de Drigalski -## Method for liquid cultures +## Método para culturas líquidas -1. Put the object slide on the table in front of you. -2. Turn on the gasburner and heat up the glass pasteur pipette. In case you are using sterile micropipette tips, just turn on the gasburner and work close to the flame. -3. Take the pipette into your primary hand and the flask with culture in the other. -4. Take out the cotton plug with the pinky finger of the hand that's holding the pipette and quickly move the opening of the flask through the flame. -5. Take up a small sample with the pipette. -6. Move the opening of the flask through the flame once more and put the plug back in. -7. Transfer the droplet onto the object slide. -8. Cover the droplet with the cover slide. -9. Dispose of any excess liquid using the paper towel. -10. Dispose of the used pasteur pipette. +1. Coloque a lâmina na mesa à sua frente. +2. Acenda a chama à gás e aqueça a pipeta pasteur de vidro. Caso você esteja usando ponteiras estéreis de micropipeta, só acenda a chama e trabalhe perto do fogo. +3. Pegue a pipeta na sua mão primária e o frasco com cultura na outra. +4. Tire o tampão de algodão com o dedo mindinho da mão que está segurando a pipeta e rapidamente mova a abertura do frasco através da chama. +5. Pegue uma pequena amostra com a pipeta. +6. Mova a abertura do frasco através da chama mais uma vez e coloque o tampão de volta. +7. Transfira a gotícula para a lâmina. +8. Cubra a amostra com a lamínula. +9. Se livre de qualquer excesso de líquido usando a toalha de papel. +10.Descarte a pipeta pasteur usada. -## Method for colonies on solid medium +## Método para colônias em meio sólido -1. Put a small droplet of water on a object slide. -2. Turn on the gasburner and heat up the inoculation loop until it's red hot. -3. Open up the petri dish and cool down the loop by touching the agar. -4. Stick the loop into the colony. -5. Swirl the loop through the droplet on the object slide. -6. Cover the droplet with the cover slide. +1. Coloque uma gota de água numa lâmina. +2. Acenda a chama e aqueça a alça de Drigalski até ficar incandescente. +3. Abra a placa de Petri e esfrie a alça encostando-a no agar. +4. Encoste a alça numa colônia. +5. Esfregue a alça na gota de água na lâmina. +6. Cubra a amostra com a lamínula. -Back to [Biofactory - Class 2: Microscope](/biofactory/class/2-microscope/) +De volta à [Biofactory - Class 2: Microscope](/biofactory/class/2-microscope/) From 4d36c80095dc6f27acf005589dd7ce463af1e2fc Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Wed, 18 Feb 2015 20:58:00 -0200 Subject: [PATCH 29/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/2/gram-staining.md | 74 ++++++++++++++--------------- 1 file changed, 37 insertions(+), 37 deletions(-) diff --git a/biofactory/class/2/gram-staining.md b/biofactory/class/2/gram-staining.md index 21f1933a47286..5d542f2aed8e6 100644 --- a/biofactory/class/2/gram-staining.md +++ b/biofactory/class/2/gram-staining.md @@ -1,57 +1,57 @@ --- layout: page -title: Gram Staining +title: Coloração de Gram permalink: /biofactory/class/2-microscope/gram-staining/ --- -## Goal +## Objetivo -Assess whether the biological sample contains Gram positive or Gram negative strains. +Avaliar se a amostra biológica contém linhagens Gram positivas ou Gram negativas. -## Materials +## Materiais -* Gas burner -* Crystal Violet -* 95% alcohol -* Iodine -* Safranin -* Tap water -* Blotting paper +* Chama a gás +* Cristal Violeta (Violeta Genciana) +* Álcool 95% +* Iodo +* Safranina +* Água de torneira +* Papel absorvente -## Method +## Método -1. Prepare a drop of sample on your microscope slide according to our [Method](/biofactory/class/2-microscope/preparing-slides/) -2. Leave the sample to air dry for 5 to 10 minutes. -3. Fixate by moving the slide back and forth through a flame for a few seconds. -4. Stain the sample by a droplet of crystal violet and let it stain for max 60 seconds. -5. Wash the crystal violet by tap water. -6. Add the iodine solution for 60 seconds. -7. Wash with tap water. -8. Decolorize using 95 % ethanol and immediately rinse with tap water. Don't let it on the slide for too long. -9. Counter stain with safranin for about 60 seconds. -10. Wash of the stain with tap water. -11. Remove excess water using the blotting paper. -12. Cover the sample with a cover slide. -13. Take a look with the microscope with full light intensity (open diaphragma) +1. Prepare uma gota da amostra numa lâmina de microscopia de acordo com nosso [Método](/biofactory/class/2-microscope/preparing-slides/) +2. Deixe a amostra secar ao ar de 5 a 10 minutos. +3. Fixe a amostra movendo a lâmina para a frente e para trás através da chama por alguns segundos. +4. Cubra a amostra com Cristal Violeta (Violeta genciana) e deixe corar por no máximo 60 segundos. +5. Lave o Cristal Violeta com água de torneira. +6. Adicione a solução de iodo por 60 segundos. +7. Lave com água de torneira. +8. Descolore usando etanol 95 % e imediatamente lave com água de torneira. Não deixe o etanol por muito tempo sobre a lâmina. +9. Adicione a safranina por 60 segundos. +10. Lave o corante com água de torneira. +11. Remova o excesso de água usando papel absorvente. +12. Cubra a amostra com a lamínula. +13. Dê uma olhada sob o microscópio com máxima intensidade de luz (abra o diafragma) -## Interpreting the results +## Interpretando os resultados -* Gram positive cells appear violet -* Gram negative cells appear red +* Células Gram positivas aparecem com cor violeta +* Células Gram negativas aparecem com cor vermelha -# Composition of the stains +# Composição dos corantes -* Crystal violet: 1 g of dry crystal violet in 10 mL 95% ethanol -* Iodine solution: 1 g of iodine in 100 mL water -* Safranin: 0.25 g of safrin in 10 mL 95% ethanol and 90 mL water +* Violeta Cristal (Violeta genciana): 1 g de cristal violeta seco iem 10 mL de etanol 95% +* Solução de Iodo: 1 g de iodo em 100 mL de água +* Safranina: 0.25 g de safranina em 10 mL de etanol 95% e 90 mL de água -![Gram Stain](/biofactory/class/2/gram-stain.jpg) +![Coloração de Gram](/biofactory/class/2/gram-stain.jpg) -Microscopic image of a Gram stain of mixed Gram-positive cocci (Staphylococcus aureus ATCC 25923, purple) and Gram-negative bacilli (Escherichia coli ATCC 11775, red). Magnification: 1.000x - Picture by [Y tambe](http://commons.wikimedia.org/wiki/File:Gram_stain_01.jpg) CC BY SA 3.0 license +Imagem microscópica de coloração de Gram de cocci Gram-positivos (Staphylococcus aureus ATCC 25923, roxo) e bacilli Gram-negativos (Escherichia coli ATCC 11775, vermelho). Aumento: 1.000x - Imagem de [Y tambe](http://commons.wikimedia.org/wiki/File:Gram_stain_01.jpg) CC BY SA 3.0 license -## Read more +## Leia mais * [Wikipedia](http://en.wikipedia.org/wiki/Gram_staining) -* [Gram Staining instruction video](https://www.youtube.com/watch?v=9Bnak_ITqck) +* [Video instrução coloração de Gram](https://www.youtube.com/watch?v=9Bnak_ITqck) -Back to [Biofactory - Class 2: Microscope](/biofactory/class/2-microscope/) +De volta à [Biofactory - Class 2: Microscope](/biofactory/class/2-microscope/) From 2b31b90c6ac052b514e8f7816dd56a3397e4bd8d Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Wed, 18 Feb 2015 21:24:18 -0200 Subject: [PATCH 30/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/4/requirements.md | 30 +++++++++++++++--------------- 1 file changed, 15 insertions(+), 15 deletions(-) diff --git a/biofactory/class/4/requirements.md b/biofactory/class/4/requirements.md index b7bac2828fb6a..64c92f8167bf4 100644 --- a/biofactory/class/4/requirements.md +++ b/biofactory/class/4/requirements.md @@ -1,30 +1,30 @@ --- layout: page -title: Sterile Hood requirements +title: Requisitos Cabine Estéril permalink: /biofactory/class/4-sterile-hood/requirements/ --- -## Functional requirements +## Requisitos Funcionais -**Must have** +**Deve-se ter** -1. Sterile working area, about the size of 10 petridishes -2. Horizontal flow +1. Área de trabalho estéril, sobre o tamanho de 10 placas de Petri +2. Fluxo horizontal -**Nice to have** +**É bom ter** -1. Adjustable airflow +1. Fluxo de ar ajustável -## Technical requirements +## Requisitos Técnicos -**Must have** +**Deve-se ter** -1. HEPA class H14 filter -2. 0.5 m2/h airflow for laminar flow -3. On/off switch +1. Filtro HEPA classe H14 +2. 0.5 m2/h fluxo de ar para fluxo laminar +3. On/off interruptor -**Nice to have** +**É bom ter** -1. Power regulator +1. Regulador de energia -Back to [Biofactory - Class 4: Sterile Hood](/biofactory/class/4-sterile-hood/) +De volta à [Biofactory - Class 4: Sterile Hood](/biofactory/class/4-sterile-hood/) From e317518c3243477f091cbb67a4b05138b159a118 Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Wed, 18 Feb 2015 21:29:30 -0200 Subject: [PATCH 31/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/5/requirements.md | 33 +++++++++++++++--------------- 1 file changed, 16 insertions(+), 17 deletions(-) diff --git a/biofactory/class/5/requirements.md b/biofactory/class/5/requirements.md index 40b7c5f6df3e6..6dd70d4c810c9 100644 --- a/biofactory/class/5/requirements.md +++ b/biofactory/class/5/requirements.md @@ -4,30 +4,29 @@ title: Centrifuge requirements permalink: /biofactory/class/5-centrifuge/requirements/ --- -## Functional requirements +## Requisitos Funcionais +**Deve-se ter** -**Must have** +1. Gaiola de segurança para rotor +2. Rotor de 8 lugares para tubo de centrífuga de 2 mL +3. Forte o suficiente para pelletar células (10,000 RPM) +4. Velocidade ajustável -1. Safe rotor cage -2. 8 place rotor for 2 mL centrifuge tube -3. Strong enough to pellet cells (10,000 RPM) -4. Adjustable speed +**É bom ter** -**Nice to have** +1. Monitor RPM -1. RPM monitor +## Requisitos Técnicos -## Technical requirements +**Deve-se ter** -**Must have** - -1. Metal or reenforcement sheet on acrylic cage -2. 3D printed rotor +1. Metal ou folha de reforço na gaiola de acrílico +2. Rotor 3D impresso 3. Brushless outer runner -4. Electronic speed controller +4. Controlador de velocidade eletrônico -**Nice to have** +**É bom ter** -1. Infra red light & sensor +1. Luz infra-vermelho & sensor -Back to [Biofactory - Class 5: Centrifuge](/biofactory/class/5-centrifuge/) +De volta à [Biofactory - Class 5: Centrifuge](/biofactory/class/5-centrifuge/) From 2e7283790c2b2e8e91a42e5b0e7c5a078a87f345 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Thu, 19 Feb 2015 10:23:47 -0200 Subject: [PATCH 32/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/continued.md | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/biofactory/continued.md b/biofactory/continued.md index bad23f07982d0..d199a7dd8ff85 100644 --- a/biofactory/continued.md +++ b/biofactory/continued.md @@ -4,11 +4,11 @@ title: Biofactory Continued permalink: /biofactory/continued/ --- -## Bioreactor extensions +## Extensões do bioreator -* pH sensor, like [SEN0169](http://dfrobot.com/wiki/index.php/Analog_pH_Meter_Pro_SKU:SEN0169) -* Water proof temp sensor, like [DS18B20](https://www.iprototype.nl/products/components/sensors/waterproof-DS18B20-digital-temp-sensor) -* Heated bed, like from [Reprapworld](http://reprapworld.com/?products/listing&cPath=_1618) -* Gas chromatography +* Sensor de pH, como esse [SEN0169](http://dfrobot.com/wiki/index.php/Analog_pH_Meter_Pro_SKU:SEN0169) +* Termistor 10K a prova d'água, como [DS18B20](https://www.iprototype.nl/products/components/sensors/waterproof-DS18B20-digital-temp-sensor) +* Mesa aquecida, como essa da [Reprapworld](http://reprapworld.com/?products/listing&cPath=_1618) +* Cromatografia a gás -Back to [Biofactory](/biofactory/) +De volta para [Biofactory](/biofactory/) From cee0877e775ea4792489b27d271d70197030ce39 Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Fri, 20 Feb 2015 11:33:14 -0200 Subject: [PATCH 33/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/4/sterile-hood-materials.md | 22 ++++++++++---------- 1 file changed, 11 insertions(+), 11 deletions(-) diff --git a/biofactory/class/4/sterile-hood-materials.md b/biofactory/class/4/sterile-hood-materials.md index 8ff3bd56687a2..d9e3a830c10ee 100644 --- a/biofactory/class/4/sterile-hood-materials.md +++ b/biofactory/class/4/sterile-hood-materials.md @@ -1,30 +1,30 @@ --- layout: page -title: Sterile Hood Materials +title: Materiais Capela Estéril permalink: /biofactory/class/4-sterile-hood/sterile-hood-materials/ --- The material list is based on the [Requirements list](/biofactory/class/4-sterile-hood/requirements/) -## Bill of Materials +## Lista de maters -This time we will not use a microprocessor. +Desta vez não iremos usar um microprocessador. -### Must have +### Deve-se ter |#|Amount|Description|Supplier NL|Cost| |-:|----:|:---------|:-------|---:| -|1|1|HEPA Filter|[OfficeStuff](http://www.officestuff.nl/producten/9370101-hepa_filter_large/)|35.82| -|2|1|250 m3/h air pump|[Webstunter](http://www.webstunter.com/ventilatie-afzuiging/centrifugaal-ventilator-afzuigmotor/direct-aangedreven-acv-centrifugaal-ventilator-afzuigmotor-250-m-h.html)|58.00| +|1|1|Filtro HEPA|[OfficeStuff](http://www.officestuff.nl/producten/9370101-hepa_filter_large/)|35.82| +|2|1|250 m3/h bomba de ar|[Webstunter](http://www.webstunter.com/ventilatie-afzuiging/centrifugaal-ventilator-afzuigmotor/direct-aangedreven-acv-centrifugaal-ventilator-afzuigmotor-250-m-h.html)|58.00| |3|1|Inline cord switch|[Gamma](https://www.gamma.nl/assortiment/gamma-snoerschakelaar-wit/p/B456235)|2.19| |4|1|Power cord|[Gamma](https://www.gamma.nl/assortiment/gamma-huishoudsnoer-rond-3x1-5-mm-wit-5-m/p/B457211)|14.29| |5|1|Wall socket power plug|[Gamma](https://www.gamma.nl/assortiment/stekker-wit/p/B306915)|1.09| |--|--|---|---|---| |||*Total costs*||| -* You will also need 3mm MDF and some M3 10 mm bolts and nuts +* Você também precisará de MDF 3mm e alguns parafusos e porcas M3 10 mm -### Nice to have +### É bom ter |#|Amount|Description|Supplier NL|Cost| |-:|----:|:---------|:-------|---:| @@ -32,8 +32,8 @@ This time we will not use a microprocessor. |--|--|---|---|---| |||*Total costs*||| -**Alternatives** +**Alternativas** -* You may choose to build a smaller hood, and go for a vacuum cleaner or car HEPA filter +* Você pode escolher construir uma capela menor, e ir para um aspirador de pó ou filtro HEPA de carro -Back to [Biofactory - Class 4: Sterile Hood](/biofactory/class/4-sterile-hood/) +De volta à [Biofactory - Class 4: Sterile Hood](/biofactory/class/4-sterile-hood/) From 9ddeedcc80ef7e339c259d4018936c5185c3f856 Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Fri, 20 Feb 2015 11:39:53 -0200 Subject: [PATCH 34/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/5/centrifuge-materials.md | 32 +++++++++++----------- 1 file changed, 16 insertions(+), 16 deletions(-) diff --git a/biofactory/class/5/centrifuge-materials.md b/biofactory/class/5/centrifuge-materials.md index 638e6f1c3fdb3..c7a1391a4dcd9 100644 --- a/biofactory/class/5/centrifuge-materials.md +++ b/biofactory/class/5/centrifuge-materials.md @@ -1,16 +1,16 @@ --- layout: page -title: Centrifuge Materials +title: Materiais Centrífuga permalink: /biofactory/class/5-centrifuge/centrifuge-materials/ --- -The material list is based on the [Requirements list](/biofactory/class/5-centrifuge/requirements/) +A lista de materiais é baseada na [Lista de Requisitor](/biofactory/class/5-centrifuge/requirements/) -## Bill of Materials +## Lista de Materiais -Make sure you have an Arduino or equivalent microprocessor. +Esteja certo de possuir um Arduino ou microprocessador equivalente. -### Must have +### Deve-se ter |#|Amount|Description|Supplier NL|Cost| |-:|----:|:---------|:-------|---:| @@ -23,24 +23,24 @@ Make sure you have an Arduino or equivalent microprocessor. |7|1|Power supply||| |8|1|Lever switch|[iPrototype](https://iprototype.nl/products/components/buttons-switches/micro-switch-right-lever) [Farnell](http://nl.farnell.com/itw-switches/19n403l18/microswitch-v4-lever/dp/624676?Ntt=624676) [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=14543)|2.33| |--|--|---|---|---| -|||*Total costs*||| +|||*Custo total*||| -* As a rotor we wil use a 3D print of your own design, or this [Microfuge 8 Place Rotor](http://www.thingiverse.com/thing:97524) -* You will also need some M3 10 mm bolts and nuts +* Como rotor nós vamos usar uma impressão 3D de seu próprio projeto, ou este [Microfuge 8 Place Rotor](http://www.thingiverse.com/thing:97524) +* Você também precisará de alguns parafusos e porcas M3 10 mm -### Nice to have +### É bom ter |#|Amount|Description|Supplier NL|Cost| |-:|----:|:---------|:-------|---:| -|1|1|Infrared sensor|[Farnell](http://nl.farnell.com/fairchild-semiconductor/qrd1114/opto-switch-reflective/dp/1467858?Ntt=1467858)|1.70| -|2|1|6.8 KOhm resistor|[Farnell](http://nl.farnell.com/multicomp/mcf-0-25w-6k8/resistor-carbon-film-6k8-0-25w/dp/9339663?Ntt=9339663)|0.02| -|3|1|220 Ohm resistor|[Farnell](http://nl.farnell.com/multicomp/mcf-0-25w-220r/resistor-carbon-film-220r-0-25w/dp/9339299?Ntt=9339299)|0.02| +|1|1|Sensor INfra-vermelho|[Farnell](http://nl.farnell.com/fairchild-semiconductor/qrd1114/opto-switch-reflective/dp/1467858?Ntt=1467858)|1.70| +|2|1|Resistor 6.8 KOhm|[Farnell](http://nl.farnell.com/multicomp/mcf-0-25w-6k8/resistor-carbon-film-6k8-0-25w/dp/9339663?Ntt=9339663)|0.02| +|3|1|Resistor 220 Ohm|[Farnell](http://nl.farnell.com/multicomp/mcf-0-25w-220r/resistor-carbon-film-220r-0-25w/dp/9339299?Ntt=9339299)|0.02| |4|1|I2C LCD display|[iPrototype](https://iprototype.nl/products/components/led-lcd/lcd16x2-I2C-BL)|16.95| |--|--|---|---|---| -|||*Total costs*||| +|||*Custo tota*||| -**Alternatives** +**Alternativas** -* Hard drive motors also speed up to about 7400 RPM +* Motores do disco rígido também aceleram até aproximadamente 7400 RPM -Back to [Biofactory - Class 5: Centrifuge](/biofactory/class/5-centrifuge/) +De volta à [Biofactory - Class 5: Centrifuge](/biofactory/class/5-centrifuge/) From fc68bc855f11c73fac7f017aeb555401140c34d1 Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Fri, 20 Feb 2015 11:40:51 -0200 Subject: [PATCH 35/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/5/requirements.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/biofactory/class/5/requirements.md b/biofactory/class/5/requirements.md index 6dd70d4c810c9..f3398328920bc 100644 --- a/biofactory/class/5/requirements.md +++ b/biofactory/class/5/requirements.md @@ -1,6 +1,6 @@ --- layout: page -title: Centrifuge requirements +title: Requisitos centrífuga permalink: /biofactory/class/5-centrifuge/requirements/ --- From 17e13cbf923991f16eef0a02131bf7b1f863f026 Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Fri, 20 Feb 2015 11:45:23 -0200 Subject: [PATCH 36/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/6/requirements.md | 22 +++++++++++----------- 1 file changed, 11 insertions(+), 11 deletions(-) diff --git a/biofactory/class/6/requirements.md b/biofactory/class/6/requirements.md index 023105a389273..3f20e1a11bf5a 100644 --- a/biofactory/class/6/requirements.md +++ b/biofactory/class/6/requirements.md @@ -1,22 +1,22 @@ --- layout: page -title: Magnetic Stirrer requirements +title: Requisitos agitador magnético permalink: /biofactory/class/6-magnetic-stirrer/requirements/ --- -## Functional requirements +## Requisitos funcionais -**Must have** +**Deve-se ter** -1. Rotating Magnets -2. Adjustable speed +1. Ímãs rotativos +2. Velocidade ajustável -## Technical requirements +## Requisitos técnicos -**Must have** +**Deve-se ter** -1. Fan -2. Permanent magnet -3. Potential meter +1. Ventilador +2. Ímã permanente +3. Medidor de potencial -Back to [Biofactory - Class 6: Magnetic Stirrer](/biofactory/class/6-magnetic-stirrer/) +De volta à [Biofactory - Class 6: Magnetic Stirrer](/biofactory/class/6-magnetic-stirrer/) From 01f3f3cdab7d7adcd22f878a65b075ab7e77e7ca Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Fri, 20 Feb 2015 12:16:19 -0200 Subject: [PATCH 37/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- .../class/6/magnetic-stirrer-materials.md | 40 +++++++++---------- 1 file changed, 20 insertions(+), 20 deletions(-) diff --git a/biofactory/class/6/magnetic-stirrer-materials.md b/biofactory/class/6/magnetic-stirrer-materials.md index cd4dd66f2014e..a56e71a6795c3 100644 --- a/biofactory/class/6/magnetic-stirrer-materials.md +++ b/biofactory/class/6/magnetic-stirrer-materials.md @@ -1,36 +1,36 @@ --- layout: page -title: Magnetic Stirrer Materials +title: Materiais Agitador Magnético permalink: /biofactory/class/6-magnetic-stirrer/stirrer-materials/ --- -The material list is based on the [Requirements list](/biofactory/class/6-magnetic-stirrer/requirements/) +A lista de materiais é baseada na [Lista de Requisitos](/biofactory/class/6-magnetic-stirrer/requirements/) -## Bill of Materials +## Lista de materiais -For this device you do not need an Arduino or equivalent microprocessor. +Para este equipamento você não precisa de um Arduino ou microprocessador equivalente. -### Must have +### Dve-se ter |#|Amount|Description|Supplier NL|Cost| |-:|----:|:---------|:-------|---:| -|1|1|Fan|[Farnell](http://nl.farnell.com/bisonic/sp802512l-03/fan-80x25mm-12vdc/dp/1832326)|8.99| -|2|2|Permanent magnets|[Supermagnete](http://www.supermagnete.nl/blokmagneten-neodymium-middelgroot/blokmagneet-10mm-x-10mm-x-5mm-neodymium-n42-vernikkeld_Q-10-10-05-N)|0.65| -|3|1|Potentiometer 100 ohm|[Farnell](http://nl.farnell.com/bourns/93r1a-r22-a05l/potentiometer-linear-100-ohm-10/dp/2321801)|6.13| -|4|1|Knob|[Farnell](http://nl.farnell.com/multicomp/cr-r4-7/knob-soft-touch-d-shaft-black/dp/1440012?ost=1440012)|0.23| -|5|1|Power switch|[iPrototype](https://iprototype.nl/products/components/buttons-switches/rocker-switch-large)|0.95| -|6|1|Power jack|[EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=3298) [iPrototype](https://iprototype.nl/products/accessoires/power/DC-barrel-jack-adapter)|0.85| -|7|1|Power supply||| -|8|1|Red LED|[iPrototype](https://iprototype.nl/products/components/led-lcd/rood#)|0.15| -|9|1|470 ohm resistor|[iPrototype](https://iprototype.nl/products/components/resistors/470R)|0.45| -|10|1|Magnetic stirring rod|[Supermagnete](http://www.supermagnete.nl/eng/rod-magnets-neodymium/rod-magnet-diameter-12mm-height-60mm-neodymium-n38-nickel-plated_S-12-60-N)|5.71| +|1|1|Ventilador|[Farnell](http://nl.farnell.com/bisonic/sp802512l-03/fan-80x25mm-12vdc/dp/1832326)|8.99| +|2|2|Ímãs permanentes|[Supermagnete](http://www.supermagnete.nl/blokmagneten-neodymium-middelgroot/blokmagneet-10mm-x-10mm-x-5mm-neodymium-n42-vernikkeld_Q-10-10-05-N)|0.65| +|3|1|Potenciômetro 100 ohm|[Farnell](http://nl.farnell.com/bourns/93r1a-r22-a05l/potentiometer-linear-100-ohm-10/dp/2321801)|6.13| +|4|1|Botão|[Farnell](http://nl.farnell.com/multicomp/cr-r4-7/knob-soft-touch-d-shaft-black/dp/1440012?ost=1440012)|0.23| +|5|1|Interruptor|[iPrototype](https://iprototype.nl/products/components/buttons-switches/rocker-switch-large)|0.95| +|6|1|Conector de alimentação|[EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=3298) [iPrototype](https://iprototype.nl/products/accessoires/power/DC-barrel-jack-adapter)|0.85| +|7|1|Fonte de energia||| +|8|1|LED Vermelho|[iPrototype](https://iprototype.nl/products/components/led-lcd/rood#)|0.15| +|9|1|Resistor 470 ohm|[iPrototype](https://iprototype.nl/products/components/resistors/470R)|0.45| +|10|1|Haste de agitação magnética|[Supermagnete](http://www.supermagnete.nl/eng/rod-magnets-neodymium/rod-magnet-diameter-12mm-height-60mm-neodymium-n38-nickel-plated_S-12-60-N)|5.71| |--|--|---|---|---| -|||*Total costs*||| +|||*Custo total*||| -* You will also need some M3 10 mm bolts and nuts +* Você também precisará de alguns parafusos e porcas M3 10 mm -**Alternatives** +**Alternativas** -* You could use all kind of motors. +* Você poderia usar todos os tipos de motores. -Back to [Biofactory - Class 6: Magnetic Stirrer](/biofactory/class/6-magnetic-stirrer/) +De volta à [Biofactory - Class 6: Magnetic Stirrer](/biofactory/class/6-magnetic-stirrer/) From dcb5c1e9b66224c07e3342172f33e20abd8d024a Mon Sep 17 00:00:00 2001 From: padilhaeduardo Date: Fri, 20 Feb 2015 12:32:03 -0200 Subject: [PATCH 38/40] =?UTF-8?q?Tradu=C3=A7=C3=A3o=20PTBR?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit --- biofactory/class/7/requirements.md | 34 +++++++++++++++--------------- 1 file changed, 17 insertions(+), 17 deletions(-) diff --git a/biofactory/class/7/requirements.md b/biofactory/class/7/requirements.md index de8d08427f1bb..01e67f6af1e87 100644 --- a/biofactory/class/7/requirements.md +++ b/biofactory/class/7/requirements.md @@ -1,33 +1,33 @@ --- layout: page -title: Spectrometer requirements +title: Requisitos spectrômetro permalink: /biofactory/class/7-spectrometer/requirements/ --- -## Functional requirements +## Requisitos funcionais -**Must have** +**Deve-se ter** -1. White light source -2. Detection range in visible light -3. Standard size cuvet holder -4. Spectrum read out +1. Fonte de luz branca +2. Faixa de detecção de luz visível +3. Suporte de cuvete de tamanho padrão +4. Leitura do espectro -**Nice to have** +**É bom ter** -1. Dedicated OD600 measurement for growth monitoring +1. Medição OD600 dedicado para o acompanhamento do crescimento -## Technical requirements +## Requisitos técnicos -**Must have** +**Deve-se ter** -1. White (full spectrum) 5V LED + suitable resistor -2. 1,000 lines/mm grating and 400 - 800 nm wavelength detection -3. 10 x 10 x 45 mm cuvet holder -4. Serial connection between device and computer +1. Branco (espectro completo) 5V LED + resistor adequado +2. Grade 1,000 linhas/mm e detecção comprimento de onda 400 - 800 nm +3. Suporte de cuvete 10 x 10 x 45 mm +4. Conexão serial entre equipamento e computador -**Nice to have** +**É bom ter** 1. I2C LCD 16x2 display -Back to [Biofactory - Class 7: Spectrometer](/biofactory/class/7-spectrometer/) +De volta à [Biofábrica - Aula 7: Espectrômetro](/biofactory/class/7-spectrometer/) From d8b24fb9442299daa5e66a06c83bddd88e71c7ed Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Mon, 23 Feb 2015 12:44:38 -0300 Subject: [PATCH 39/40] Update requirements.md --- biofactory/class/4/requirements.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/biofactory/class/4/requirements.md b/biofactory/class/4/requirements.md index 64c92f8167bf4..4d2a65534f14c 100644 --- a/biofactory/class/4/requirements.md +++ b/biofactory/class/4/requirements.md @@ -8,7 +8,7 @@ permalink: /biofactory/class/4-sterile-hood/requirements/ **Deve-se ter** -1. Área de trabalho estéril, sobre o tamanho de 10 placas de Petri +1. Área de trabalho estéril, para caber cerca de 10 placas de Petri 2. Fluxo horizontal **É bom ter** @@ -21,7 +21,7 @@ permalink: /biofactory/class/4-sterile-hood/requirements/ 1. Filtro HEPA classe H14 2. 0.5 m2/h fluxo de ar para fluxo laminar -3. On/off interruptor +3. Interruptor liga/desliga **É bom ter** From eeb69cd871a4ef0a9b5da998f28f6518c794a079 Mon Sep 17 00:00:00 2001 From: Garagem Fab Lab Date: Mon, 23 Feb 2015 12:46:48 -0300 Subject: [PATCH 40/40] Update sterile-hood-materials.md --- biofactory/class/4/sterile-hood-materials.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/biofactory/class/4/sterile-hood-materials.md b/biofactory/class/4/sterile-hood-materials.md index d9e3a830c10ee..87ba698d7540d 100644 --- a/biofactory/class/4/sterile-hood-materials.md +++ b/biofactory/class/4/sterile-hood-materials.md @@ -6,7 +6,7 @@ permalink: /biofactory/class/4-sterile-hood/sterile-hood-materials/ The material list is based on the [Requirements list](/biofactory/class/4-sterile-hood/requirements/) -## Lista de maters +## Lista de materiais Desta vez não iremos usar um microprocessador. @@ -34,6 +34,6 @@ Desta vez não iremos usar um microprocessador. **Alternativas** -* Você pode escolher construir uma capela menor, e ir para um aspirador de pó ou filtro HEPA de carro +* Você pode escolher construir uma capela menor e utilizar um aspirador de pó ou filtro HEPA de carro. De volta à [Biofactory - Class 4: Sterile Hood](/biofactory/class/4-sterile-hood/)