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Although stk does respect IsoSMILES stereochemistry, it would be nice to validate the chirality of constructed molecules as a sanity check. It should be pretty straightforward via RDKit substructure matching with flag useChirality-True e.g. len(constructed_macrocycle.GetSubstructMatches(dextrose, useChirality=True)), but since dextrose is not entirely present in cyclodextrin, you'll have to use some common sub-structure instead.
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