iscoSNP++ treating paired-end reads as separate samples / FOF format confusion

[Gurnur-Kaur]

I have 2 biological samples (glabra_01, uncata_01), each with R1+R2 Paired end reads.

reads.fof (4 lines):
/path/glabra_01_R1.fastq.gz
/path/glabra_01_R2.fastq.gz
/path/uncata_01_R1.fastq.gz
/path/uncata_01_R2.fastq.gz

→ VCF shows G1,G2,G3,G4 (4 samples)

I even tried giving path as follows (each sample forward and reverse reads on same line separated by space):
/path/glabra_01_R1.fastq.gz /path/glabra_01_R2.fastq.gz
/path/uncata_01_R1.fastq.gz /path/uncata_01_R2.fastq.gz
but in this case command-run_discoSnp++.sh -r reads.fof -p trig_denovo_snps -k 31 -c 5 -b 1 -P 2 -u 4
doesn't execute ,file accessibility error

I want: S1=glabra_01, S2=uncata_01 (2 samples) in vcf file

Command used:
run_discoSnp++.sh -r reads.fof -p trig_test -k 31 -c 5 -b 1 -P 2 -u 4

Question: How to format fof correctly?