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Course

Repository for course files

Session 1

Galaxy
- Introduction
- Transcriptomics
  - Data loading
  - Quality control
  - Read trimming
  - Alignment
  - Counting features
  - DESeq2

Session 2

R & RStudio
- Download and install
- Using R notebooks
- Introduction to R and Tidyverse

Session3

DESeq2 - RStudio
- Reading in data
- Experimantal design
- Normalisation
- Differential expression

Session 4

Visualising DESeq2 results
- ggplot2
  - PCA plots
  - Dispersion plots
  - Heatmaps
- Contrasts
  - Volcano plots
  - MA plots

Session 5

Pathway analysis
Gene Ontology analysis