diff --git a/luminex/src/org/labkey/luminex/LuminexUploadWizardAction.java b/luminex/src/org/labkey/luminex/LuminexUploadWizardAction.java
index c161d9800..97dd877cf 100644
--- a/luminex/src/org/labkey/luminex/LuminexUploadWizardAction.java
+++ b/luminex/src/org/labkey/luminex/LuminexUploadWizardAction.java
@@ -441,14 +441,16 @@ public void writeSameCheckboxCell(RenderContext ctx, HtmlWriter out)
                         InputBuilder.checkbox().name(id).id(id)
                     ).appendTo(out);
 
-                    StringBuilder onchange = new StringBuilder("b = this.checked;");
+                    StringBuilder onchange = new StringBuilder("b = this.checked;\n");
                     // Index starts at 1 -- always leave the first column visible (Issue 53620)
                     for (int i = 1; i < getColumns().size(); i++)
                     {
                         DisplayColumn col = getColumns().get(i);
-                        onchange.append("document.getElementsByName('").append(col.getFormFieldName(ctx)).append("')[0].style.display = b ? 'none' : 'block';\n");
+                        // Issue 53620: instead of hiding the input, set it "disabled" via CSS (but not actually disabled so it will still submit)
+                        onchange.append("document.getElementsByName('").append(col.getFormFieldName(ctx)).append("')[0].style.opacity = b ? 0.6 : 1;\n");
+                        onchange.append("document.getElementsByName('").append(col.getFormFieldName(ctx)).append("')[0].style.pointerEvents = b ? 'none' : 'all';\n");
                     }
-                    onchange.append("if (b) { ").append(groupName).append("Updated(); }");
+                    onchange.append("if (b) { ").append(groupName).append("Updated(); }\n");
                     HttpView.currentPageConfig().addHandler(id, "change", onchange.toString());
                 }
 
diff --git a/luminex/src/org/labkey/luminex/query/NegativeBeadDisplayColumnGroup.java b/luminex/src/org/labkey/luminex/query/NegativeBeadDisplayColumnGroup.java
index 7bde52e82..115940e55 100644
--- a/luminex/src/org/labkey/luminex/query/NegativeBeadDisplayColumnGroup.java
+++ b/luminex/src/org/labkey/luminex/query/NegativeBeadDisplayColumnGroup.java
@@ -55,18 +55,15 @@ public void writeSameCheckboxCell(RenderContext ctx, HtmlWriter out)
                     DisplayColumn col = getColumns().get(i);
                     if (col.getColumnInfo() != null)
                     {
-                        onChange.append("s = document.getElementsByName('")
-                            .append(col.getFormFieldName(ctx))
-                            .append("')[0].options.length;\n")
-                            .append("document.getElementsByName('")
-                            .append(col.getFormFieldName(ctx))
-                            .append("')[0].style.display = b || s == 0 ? 'none' : 'block';\n");
+                        // Issue 53620: instead of hiding the input, set it "disabled" via CSS (but not actually disabled so it will still submit)
+                        onChange.append("document.getElementsByName('").append(col.getFormFieldName(ctx)).append("')[0].style.opacity = b ? 0.6 : 1;\n");
+                        onChange.append("document.getElementsByName('").append(col.getFormFieldName(ctx)).append("')[0].style.pointerEvents = b ? 'none' : 'all';\n");
                     }
                 }
 
                 onChange.append(" if (b) { ")
                     .append(inputName)
-                    .append("Updated(); }");
+                    .append("Updated(); }\n");
                 HttpView.currentPageConfig().addHandler(id, "change", onChange.toString());
 
                 return ret;
diff --git a/luminex/test/src/org/labkey/test/tests/luminex/LuminexRTransformTest.java b/luminex/test/src/org/labkey/test/tests/luminex/LuminexRTransformTest.java
index a2a0ec3a1..4b3f1489d 100644
--- a/luminex/test/src/org/labkey/test/tests/luminex/LuminexRTransformTest.java
+++ b/luminex/test/src/org/labkey/test/tests/luminex/LuminexRTransformTest.java
@@ -132,8 +132,7 @@ public void uploadRun()
         // make sure the Standard checkboxes are checked
         checkCheckbox(Locator.name("_titrationRole_standard_Standard1"));
         checkCheckbox(Locator.name("titration_" + ANALYTE1 + "_Standard1"));
-        checkCheckbox(Locator.name("titration_" + ANALYTE2 + "_Standard1"));
-        checkCheckbox(Locator.name("titration_" + ANALYTE3 + "_Standard1"));
+        checkCheckbox(Locator.name("titration_" + ANALYTE1 + "_Standard1CheckBox")); // Issue 53620: "Same" checkbox for standards
         // make sure that that QC Control checkbox is checked
         checkCheckbox(Locator.name("_titrationRole_qccontrol_Standard1"));
         // set LotNumber for the first analyte