2025/06/15/Everything-you-shall-know-when-using-%CE%94%CE%94Ct-method-in-qPCR

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Everything you shall know when using ΔΔCt method in qPCR - BSGOU

🌐 Other languages: English 日本語Have you ever run into this situation while using the ΔΔCt method for qPCR analysis—your control group’s mean expression value ...

https://www.bs-gou.com/2025/06/15/Everything-you-shall-know-when-using-%CE%94%CE%94Ct-method-in-qPCR.html

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💬 Questions, feedback, or even typo reports are very welcome!
Science moves forward through transparency and conversation.

🧪 Have you ever performed a secondary normalization when using the ΔΔCt method?
📊 Geometric vs arithmetic mean — which one do you use in your qPCR analysis?
Is it a statistical choice, or just lab tradition?

🗨️ Share your experience or thoughts below 👇