diff --git a/single_cell/RNAseq/dgeMAST_function.R b/single_cell/RNAseq/dgeMAST_function.R
new file mode 100644
index 0000000..b9f5b59
--- /dev/null
+++ b/single_cell/RNAseq/dgeMAST_function.R
@@ -0,0 +1,238 @@
+##### This script initializes a function called 'dgeMAST' for running differential gene expression
+# This is an adaptation by Dan Bunis of code originally formulated by Ravi Patel
+#
+# The function loop across multiple clusters running DGE between 2 groups, gathering eveything into a single data.frame.
+# Think like FindAllMarkers, but for a biological question per each cluster.
+#
+# Some key notes:
+# - This is a method meant for droplet-based single-cell transcriptome data.
+# - Raw counts (integers) are expected to exist and to be:
+#   - in the 'counts' slot of a Seurat object's 'RNA' assay.
+#   - in an assay named 'counts' if using a SingleCellExperiment object.
+#
+# To use, `source("path/to/this/file")`
+# Then, something like:
+# dge <- dgeMAST(
+#   object = sobj, # Seurat or SCE object
+#   cells.group.by = 'full_annots', # metadata name holding clusters or annotations to explore within
+#   dge.group.by = 'sex_at_birth', # metadata name holding the samples'/cell's groupings to compare across
+#   dge.group.1 = 'M', # value of 'dge.group.by' metadata value of cells in the primary group.
+#   dge.group.2 = 'F', # value of 'dge.group.by' metadata value of cells in the secondary / control group.
+#   cells.group.targets = c('T4 naive', 'T8 em') # Without this, it will run within ALL cell types / clusters (a.k.a. all 'full_annots' per this example)
+# )
+# See roxygen notes below for additional details!
+
+suppressPackageStartupMessages({
+  library(assertthat)
+  library(dplyr)
+  library(Seurat)
+  library(SingleCellExperiment)
+  library(MAST)
+  library(data.table)
+  library(dittoSeq)
+})
+
+#' Run DGE between 2 groups while looping through cell types
+#' @param object A Seurat or SingleCellExperiment (SCE) object
+#' @param cells.group.by String naming a metadata column of \code{object} which holds clusters, annotations, or other cell groupings to explore within
+#' @param cells.group.targets NULL or a string vector naming particular levels of the \code{cell.group.by}-data to run DGE within.  When left as \code{NULL}, all cell groupings will be targeted
+#' @param dge.group.by String naming a metadata column of \code{object} which holds the sample or cell groupings that you wish to compare between
+#' @param dge.group.1,dge.group.2 Strings naming the groups of \code{dge.group.by}-data to compare between. Directionality: Positive log2FC (foldChange) will mean upregulation in \code{dge.group.1} with respect to \code{dge.group.2}.
+#' @param cells.use NULL or a Logical vector (of length equal to the number of cells in \code{object}) indicating cells to keep (TRUE) or remove (FALSE) before calculating DGE. Can be useful for ignoring specific samples.
+#' @param log.prefix String to append at the beginning of log messages. Useful when wrapping this function inside an external loop.
+#' @param mast.freq.expressed.min A fraction between 0 and 1, default = 0.2, which sets the minimal percent of cells that must express a gene for it to be considered. MAST performs less well for genes with low percent expression. This cutoff is run per each considered \code{cell.group.targets} cell grouping, and only expression among cells of the targeted \code{dge.group.1} and \code{dge.group.2} groups are considered.
+#' @param add.random.effect,add.fixed.effect # NULL or String vectors naming metadata columns of \code{object} to treat as a random or fixed effects, respectively, in the mixed effect model used for DGE calculation. Additional details:
+#' \itemize{
+#' \item NOTE: \code{add.random.effect} defaults to "orig.ident" with the assumption that this column generally 1) exists and 2) holds batch information that is normally desired to be modeled as a random effect. Set to NULL, or something different, to turn this off.
+#' \item \code{add.random.effect} must target discrete metadata and will be turned into a factor before use.
+#' \item 'ngeneson' (the number of expressed genes per cell, which will be calculated internally) and \code{dge.group.by} are automatically added as fixed effects and do not need to be added here.
+#' }
+#'
+#' @return a data.table
+#' @details This function loops across multiple clusters running DGE between 2 groups, gathering eveything into a single data.frame.
+#' Think like FindAllMarkers, but for a biological question per each cluster.
+#'
+#' Raw counts (integers) are expected to exist and to be in the 'counts' slot of the 'RNA' assay if \code{object} is a Seurat object, or in an assay named 'counts' if \code{object} is a SingleCellExperiment object.
+#'
+#' In preparation:
+#'
+#' Seurat data is converted to a SingleCellObject (SCE).
+#' The SCE is trimmed to only the cells indicated by \code{cells.use}, and only those where \code{dge.group.by} is either \code{dge.group.1} or \code{dge.group.2}.
+#' And the set of cell targets given by \code{cells.group.targets} is trimmed of any targets that don't have any cells of code{dge.group.1} or \code{dge.group.2}
+#'
+#' Then, the function loops through \code{cells.group.targets}, performing these steps per each subset of cells:
+#'
+#' Log and read-depth normalization of the counts data,
+#' trimming genes expressed in fewer than \code{mast.freq.expressed.min} proportion of cells,
+#' calculation of the number of genes expressed per cell ('ngeneson'),
+#' the DGE calculation with \code{formula} based on the contents of \code{add.random.effect} and \code{add.fixed.effect}.
+#' By default, the formula amounts to \code{~ <dge.group.by> + ngeneson + (1 | orig.ident)}.
+#' FDR-correction is applied to p-values at this stage -- per each cell type.
+#'
+#' It then renames columns, converts the column holding fold change information to be base2, and adds an "up_in" column to help explain which code{dge.group.1} or \code{dge.group.2} was found to have higher expression for each gene.
+#' @section DGE methodology details:
+#' DGE is run by first modeling the data using MAST's zlm function, followed by determining p-values using its likelihood ratio test methodology on the contrast of interest.
+#'
+#' Specifically, \code{zlm} is run with settings: \itemize{
+#' \item Always: \code{ebayes = FALSE, strictConvergence = FALSE}
+#' \item Additionally:\itemize{
+#'   \item when there is a random effect given (default) to \code{add.random.effect}: \code{method='glmer', fitArgsD = list(nAGQ = 0)}
+#'   \item when there is no random effect given to \code{add.random.effect}: \code{method='glm'}
+#' }
+#' }
+#' @author Daniel Bunis and Ravi Patel
+dgeMAST <- function(
+  object,
+  cells.group.by,
+  cells.group.targets = NULL,
+  dge.group.by,
+  dge.group.1,
+  dge.group.2,
+  cells.use = NULL,
+  log.prefix = '',
+  mast.freq.expressed.min = 0.2,
+  add.random.effect = "orig.ident",
+  add.fixed.effect = NULL
+) {
+
+  log_message <- function(...) {
+    cat("[", format(Sys.time()), "]", ..., "\n", sep = "")
+  }
+
+  if (!dittoSeq::isMeta(cells.group.by, object)) stop("'cells.group.by', ", cells.group.by,", is not a metadata of 'object'")
+  if (!dittoSeq::isMeta(dge.group.by, object)) stop("'dge.group.by', ", dge.group.by,", is not a metadata of 'object'")
+  if (!dge.group.1 %in% object[[dge.group.by, drop = TRUE]]) stop("No cells have 'dge.group.1', ", dge.group.1, ", as their 'dge.group.by' value.")
+  if (!dge.group.2 %in% object[[dge.group.by, drop = TRUE]]) stop("No cells have 'dge.group.2', ", dge.group.2, ", as their 'dge.group.by' value.")
+  if (!is.null(cells.group.targets) && !all(cells.group.targets %in% object[[cells.group.by, drop = TRUE]])) stop("At least one target of 'cells.group.targets' does not exist within 'cells.group.by' values.")
+
+  # Tranform minimal bits into an SCE
+  if (!is(object, "SingleCellExperiment")) {
+    sce <- as.SingleCellExperiment(DietSeurat(object, assays="RNA"), assay="RNA")
+  } else {
+    sce <- object
+  }
+
+  # Trim to only cells passing cells.use
+  if (!identical(cells.use, NULL)) {
+    sce <- sce[, dittoSeq:::.which_cells(
+      cells.use,
+      sce)]
+  }
+
+  # Trim to only target dge.groups, and use factor levels to have it target the right groups.
+  sce <- sce[, dittoSeq:::.which_cells(
+    meta(dge.group.by, sce) %in% c(dge.group.1, dge.group.2),
+    sce)]
+  sce[[dge.group.by]] <- factor(
+    meta(dge.group.by, sce),
+    levels = c(dge.group.2, dge.group.1)
+  )
+
+  # Establish cell targets if not given
+  if (is.null(cells.group.targets)) {
+    cells.group.targets <- dittoSeq::metaLevels(cells.group.by, sce)
+  }
+
+  # Check for any lack of cell groups, and trim cell targets if so
+  rms <- c()
+  grps <- dittoSeq::meta(dge.group.by, sce)
+  cts <- as.character(dittoSeq::meta(cells.group.by, sce))
+  for (cell_targ in cells.group.targets) {
+    if (any(table(grps[cts==cell_targ])==0)) {
+      log_message(log.prefix, "Skipping cell type ", cell_targ, " because a dge.group has no cells")
+      rms <- c(rms, cell_targ)
+    }
+  }
+  cells.group.targets <- cells.group.targets[!cells.group.targets %in% rms]
+  rm(cell_targ)
+
+  # Loop through cell types, building dge output for each
+  dge_all_list <- lapply(
+    cells.group.targets,
+    function(cell_targ) {
+
+      log_message(log.prefix, "Running MAST dge for ", cell_targ, " cells")
+      # Trim to just these cells
+      sce_ct <- sce[, dittoSeq:::.which_cells(
+        meta(cells.group.by, sce) == cell_targ,
+        sce)]
+
+      counts <- assay(sce_ct, "counts")
+      libsizes <- colSums(counts)
+      size.factors <- libsizes/mean(libsizes)
+      logcounts(sce_ct) <- log2(t(t(counts)/size.factors) + 1)
+
+      ### Ensure logcounts comes first!  ...by simply removing all others.
+      for (assay in assayNames(sce_ct)) {
+        if (assay!="logcounts") {
+          assay(sce_ct, assay) <- NULL
+        }
+      }
+      stopifnot(assayNames(sce_ct)[1]=="logcounts")
+
+      # Transform into MAST's required SingleCellAssay structure.
+      sca_ct <- SceToSingleCellAssay(sce_ct)
+
+      # Trim to only genes expressed in enough cells
+      expressed_genes <- freq(sca_ct) > mast.freq.expressed.min
+      sca_ct <- sca_ct[expressed_genes,]
+
+      # Add meta data for num genes captured per cell
+      cdr2_ct <- colSums(assay(sca_ct)>0)
+      colData(sca_ct)$ngeneson <- scale(cdr2_ct)
+
+      # Build model
+      model <- paste0("~ ngeneson + ", dge.group.by)
+      # Ensure random effects, like batch, are factors too
+      for (rand in add.random.effect) {
+        colData(sca_ct)[,rand] <- as.factor(colData(sca_ct)[,rand])
+        model <- paste0(model, " + (1 | ", rand, ")")
+      }
+      for (fix in add.fixed.effect) {
+        model <- paste0(model, " + fixed")
+      }
+
+      # Run MAST
+      zlm_args <- list(
+          formula = as.formula(model),
+          sca = sca_ct,
+          exprs_values = 'logcounts',
+          ebayes = FALSE,
+          strictConvergence = FALSE,
+          method = 'glm'
+      )
+      if (!identical(add.random.effect, NULL)) {
+          zlm_args$method = 'glmer'
+          zlm_args$fitArgsD = list(nAGQ = 0)
+      }
+
+      log_message(log.prefix, "\twith model formula: ", model)
+      contrast_val <- paste0(dge.group.by, dge.group.1)
+      zlmCond <- do.call(zlm, zlm_args)
+      summaryCond <- summary(zlmCond, doLRT=contrast_val)$datatable
+      fcHurdle <- merge(
+        summaryCond[contrast==contrast_val & component=='H',.(primerid, `Pr(>Chisq)`)], #hurdle P values
+        summaryCond[contrast==contrast_val & component=='logFC', .(primerid, coef, ci.hi, ci.lo)], by='primerid') #logFC coefficients
+      fcHurdle[,fdr:=p.adjust(`Pr(>Chisq)`, 'fdr')]
+
+      # Rebase coef toward log2() instead of ln()
+      fcHurdle$coef <- fcHurdle$coef / log(2, base = exp(1))
+
+      # Rename columns
+      names(fcHurdle)[1:3] <- c("gene", "p", "log2FC")
+
+      fcHurdle$cell_group <- cell_targ
+      fcHurdle$up_in <- ifelse(
+          fcHurdle$log2FC>0, dge.group.1,
+          ifelse(fcHurdle$log2FC<0, dge.group.2, NA)
+      )
+
+      # Return
+      fcHurdle
+    }
+  )
+
+  # Collapse tables from all cell types into one, and return!
+  do.call(rbind, dge_all_list)
+}
+
diff --git a/single_cell/RNAseq/dgeMAST_function.txt b/single_cell/RNAseq/dgeMAST_function.txt
new file mode 100644
index 0000000..0c3335d
--- /dev/null
+++ b/single_cell/RNAseq/dgeMAST_function.txt
@@ -0,0 +1,141 @@
+Run DGE between 2 groups while looping through cell types
+
+Description:
+
+     Run DGE between 2 groups while looping through cell types
+
+Usage:
+
+     dgeMAST(
+       object,
+       cells.group.by,
+       cells.group.targets = NULL,
+       dge.group.by,
+       dge.group.1,
+       dge.group.2,
+       cells.use = NULL,
+       log.prefix = "",
+       mast.freq.expressed.min = 0.2,
+       add.random.effect = "orig.ident",
+       add.fixed.effect = NULL
+     )
+     
+Arguments:
+
+  object: A Seurat or SingleCellExperiment (SCE) object
+
+cells.group.by: String naming a metadata column of ‘object’ which holds
+          clusters, annotations, or other cell groupings to explore
+          within
+
+cells.group.targets: NULL or a string vector naming particular levels
+          of the ‘cell.group.by’-data to run DGE within.  When left as
+          ‘NULL’, all cell groupings will be targeted
+
+dge.group.by: String naming a metadata column of ‘object’ which holds
+          the sample or cell groupings that you wish to compare between
+
+dge.group.1, dge.group.2: Strings naming the groups of
+          ‘dge.group.by’-data to compare between. Directionality:
+          Positive log2FC (foldChange) will mean upregulation in
+          ‘dge.group.1’ with respect to ‘dge.group.2’.
+
+cells.use: NULL or a Logical vector (of length equal to the number of
+          cells in ‘object’) indicating cells to keep (TRUE) or remove
+          (FALSE) before calculating DGE. Can be useful for ignoring
+          specific samples.
+
+log.prefix: String to append at the beginning of log messages. Useful
+          when wrapping this function inside an external loop.
+
+mast.freq.expressed.min: A fraction between 0 and 1, default = 0.2,
+          which sets the minimal percent of cells that must express a
+          gene for it to be considered. MAST performs less well for
+          genes with low percent expression. This cutoff is run per
+          each considered ‘cell.group.targets’ cell grouping, and only
+          expression among cells of the targeted ‘dge.group.1’ and
+          ‘dge.group.2’ groups are considered.
+
+add.random.effect, add.fixed.effect: # NULL or String vectors naming
+          metadata columns of ‘object’ to treat as a random or fixed
+          effects, respectively, in the mixed effect model used for DGE
+          calculation. Additional details:
+
+            • NOTE: ‘add.random.effect’ defaults to "orig.ident" with
+              the assumption that this column generally 1) exists and
+              2) holds batch information that is normally desired to be
+              modeled as a random effect. Set to NULL, or something
+              different, to turn this off.
+
+            • ‘add.random.effect’ must target discrete metadata and
+              will be turned into a factor before use.
+
+            • 'ngeneson' (the number of expressed genes per cell, which
+              will be calculated internally) and ‘dge.group.by’ are
+              automatically added as fixed effects and do not need to
+              be added here.
+
+Details:
+
+     This function loops across multiple clusters running DGE between 2
+     groups, gathering eveything into a single data.frame. Think like
+     FindAllMarkers, but for a biological question per each cluster.
+
+     Raw counts (integers) are expected to exist and to be in the
+     'counts' slot of the 'RNA' assay if ‘object’ is a Seurat object,
+     or in an assay named 'counts' if ‘object’ is a
+     SingleCellExperiment object.
+
+     In preparation:
+
+     Seurat data is converted to a SingleCellObject (SCE). The SCE is
+     trimmed to only the cells indicated by ‘cells.use’, and only those
+     where ‘dge.group.by’ is either ‘dge.group.1’ or ‘dge.group.2’. And
+     the set of cell targets given by ‘cells.group.targets’ is trimmed
+     of any targets that don't have any cells of codedge.group.1 or
+     ‘dge.group.2’
+
+     Then, the function loops through ‘cells.group.targets’, performing
+     these steps per each subset of cells:
+
+     Log and read-depth normalization of the counts data, trimming
+     genes expressed in fewer than ‘mast.freq.expressed.min’ proportion
+     of cells, calculation of the number of genes expressed per cell
+     ('ngeneson'), the DGE calculation with ‘formula’ based on the
+     contents of ‘add.random.effect’ and ‘add.fixed.effect’. By
+     default, the formula amounts to ‘~ <dge.group.by> + ngeneson + (1
+     | orig.ident)’. FDR-correction is applied to p-values at this
+     stage - per each cell type.
+
+     It then renames columns, converts the column holding fold change
+     information to be base2, and adds an "up_in" column to help
+     explain which codedge.group.1 or ‘dge.group.2’ was found to have
+     higher expression for each gene.
+
+Value:
+
+     a data.table
+
+DGE methodology details:
+
+     DGE is run by first modeling the data using MAST's zlm function,
+     followed by determining p-values using its likelihood ratio test
+     methodology on the contrast of interest.
+
+     Specifically, ‘zlm’ is run with settings:
+
+        • Always: ‘ebayes = FALSE, strictConvergence = FALSE’
+
+        • Additionally:
+
+            • when there is a random effect given (default) to
+              ‘add.random.effect’: ‘method='glmer', fitArgsD =
+              list(nAGQ = 0)’
+
+            • when there is no random effect given to
+              ‘add.random.effect’: ‘method='glm'’
+
+Author(s):
+
+     Daniel Bunis and Ravi Patel
+