From c62e9bce2760adee6aec8711443898e6fa6c61e0 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Javier=20Mill=C3=A1n=20Acosta?= <83466805+jmillanacosta@users.noreply.github.com> Date: Mon, 9 Mar 2026 10:51:54 +0100 Subject: [PATCH 1/5] Change assignee for new method entry template --- .github/ISSUE_TEMPLATE/new-tool-method-entry.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/ISSUE_TEMPLATE/new-tool-method-entry.yml b/.github/ISSUE_TEMPLATE/new-tool-method-entry.yml index 30409939..4195418c 100644 --- a/.github/ISSUE_TEMPLATE/new-tool-method-entry.yml +++ b/.github/ISSUE_TEMPLATE/new-tool-method-entry.yml @@ -2,7 +2,7 @@ name: New Method Catalogue Entry description: Request to add a new method to the catalogue title: "[METHOD]: " labels: ["method"] -assignees: jmillanacosta +assignees: ShakiraPortfolio body: - type: markdown From 2d1e49e80cba55f9b5f17073d13a92b01229df9d Mon Sep 17 00:00:00 2001 From: jmillanacosta <83466805+jmillanacosta@users.noreply.github.com> Date: Mon, 9 Mar 2026 09:56:34 +0000 Subject: [PATCH 2/5] Add method JSON for issue #243 --- docs/methods/resazurine_assay.json | 25 +++++++++++++++++++++++++ 1 file changed, 25 insertions(+) create mode 100644 docs/methods/resazurine_assay.json diff --git a/docs/methods/resazurine_assay.json b/docs/methods/resazurine_assay.json new file mode 100644 index 00000000..47b2a6ad --- /dev/null +++ b/docs/methods/resazurine_assay.json @@ -0,0 +1,25 @@ +{ + "id": "resazurine_assay", + "method": "Resazurine assay", + "issue_number": 243, + "assay_name_content": "Resazurine assay", + "data_producer_content": "Unknown at this timepoint", + "available_sop_or_protocol_content": "_No response_", + "method_description_content": "Fluorometric method to measure cell viability through enzymatic reduction of resazurin to resorufin by viable cells", + "case_study_content": "kidney", + "http": "//purl.obolibrary.org/obo/OBI_0003757", + "ontology_term_url": "http://purl.obolibrary.org/obo/OBI_0003757", + "regulatory_question_content": "Q2", + "type_content": "ontology", + "relevant_vhp4safety_regulatory_question(s)_content": "Kidney Disease (b)", + "relevant_aop_wiki_key_event(s)_to_the_assay_content": "_No response_", + "relevant_aop_wiki_adverse_outcome_pathway(s)_to_the_assay_content": "_No response_", + "vendor_content": "ThermoFisher Scientific", + "catalog_number_content": "V23110", + "https": "//documents.thermofisher.com/TFS-Assets/LSG/manuals/mp23110.pdf", + "catalog_webpage_url": "https://www.thermofisher.com/order/catalog/product/V23110?SID=srch-srp-V23110", + "citation_url": "https://documents.thermofisher.com/TFS-Assets/LSG/manuals/mp23110.pdf", + "vhp4safety_workflow_stage_content": "AOP", + "workflow_substage_content": "Effects on Key Events", + "timestamp": "2026-03-09T09:56:34.458233Z" +} \ No newline at end of file From 75d9aa7d7bb0fb27a0b6a9f079f8cb8343337ea3 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Javier=20Mill=C3=A1n=20Acosta?= <83466805+jmillanacosta@users.noreply.github.com> Date: Mon, 9 Mar 2026 11:04:35 +0100 Subject: [PATCH 3/5] Update README with method and service addition guidelines Added instructions for requesting new methods and updating services. --- README.md | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/README.md b/README.md index e539ea3c..45dbc95d 100644 --- a/README.md +++ b/README.md @@ -3,6 +3,10 @@ Repository to document the services on the VHP4Safety Cloud, see this [index](docs/index.md) or the [online website](https://vhp4safety.github.io/cloud/). +## Adding new methods +* Post an issue requesting a new method +* For maintainers: add the [`method-approved-for-pr` label](https://github.com/VHP4Safety/cloud/issues?q=is%3Aissue%20state%3Aopen%20label%3Amethod-approved-for-pr) when the generated JSON looks right. + ## Adding new services * copy the `docs/services/template.json` file and adapt the content to the new service From 646409fc36e1ed92461d9da5150e243414964ed8 Mon Sep 17 00:00:00 2001 From: "github-actions[bot]" Date: Mon, 9 Mar 2026 10:12:12 +0000 Subject: [PATCH 4/5] Auto-generate methods index --- docs/methods/index.md | 6 +++++- 1 file changed, 5 insertions(+), 1 deletion(-) diff --git a/docs/methods/index.md b/docs/methods/index.md index 927b8372..4e87bb59 100644 --- a/docs/methods/index.md +++ b/docs/methods/index.md @@ -8,7 +8,7 @@ This catalog contains experimental methods and protocols used in VHP4Safety rese [Request a new method](https://github.com/VHP4Safety/cloud/issues/new/choose) | [Browse all methods](https://github.com/VHP4Safety/cloud/tree/main/docs/methods) -## Available Methods (18) +## Available Methods (19) ### AOP Methods @@ -44,6 +44,10 @@ mitochondrial membrane potential assay (Vendor: Thermofisher) [[more information Fluorescent-based assay to label mitochondria (Vendor: ThermoFisher Scientific) [[more information](mitotracker_deep_red_fm_assay.md)] +#### Resazurine assay + +Fluorometric method to measure cell viability through enzymatic reduction of resazurin to resorufin by viable cells (Vendor: ThermoFisher Scientific) [[more information](resazurine_assay.md)] + ### AOP, Adverse Outcome Methods #### Cytotoxicity Detection KitPLUS (LDH) From a1c1375bfaea1814948d43164489e54d1ed0eb34 Mon Sep 17 00:00:00 2001 From: GitHub Action Date: Mon, 9 Mar 2026 10:12:19 +0000 Subject: [PATCH 5/5] Generate method documentation from JSON files --- docs/methods/resazurine_assay.md | 45 ++++++++++++++++++++++++++++++++ 1 file changed, 45 insertions(+) create mode 100644 docs/methods/resazurine_assay.md diff --git a/docs/methods/resazurine_assay.md b/docs/methods/resazurine_assay.md new file mode 100644 index 00000000..1e7328bb --- /dev/null +++ b/docs/methods/resazurine_assay.md @@ -0,0 +1,45 @@ +# Resazurine assay + + + +Fluorometric method to measure cell viability through enzymatic reduction of resazurin to resorufin by viable cells + +## Method Details + +* **Vendor:** ThermoFisher Scientific + +* **Catalog Number:** V23110 + +* **Catalog Webpage:** [https://www.thermofisher.com/order/catalog/product/V23110?SID=srch-srp-V23110](https://www.thermofisher.com/order/catalog/product/V23110?SID=srch-srp-V23110) + +* **VHP4Safety Workflow Stage:** AOP + +* **Workflow Substage:** Effects on Key Events + +* **Case Study:** kidney + +* **Regulatory Question:** Q2 + +## Additional Information + +* **Assay Name:** Resazurine assay + +* **Data Producer:** Unknown at this timepoint + +* **Relevant AOP Wiki Key Event(s):** _No response_ + +* **Relevant AOP Wiki Adverse Outcome Pathway(s):** _No response_ + +* **Available SOP or Protocol:** _No response_ + +* **Ontology Term:** Not available + +* **Citation:** Not available + +--- + +*Generated from issue #243* + +