input more sequences, get their primers separately!

[qiuqingpo]

Hi David, Thanks for developing DNAWorks and for making it open source! I want to input a set of sequences and then get their primers separately, but now the program will automatically merge them into one sequence. I have calculated their primers as a whole, please tell me how to change them most appropriately? Thank you

[guozheng-git]

Hi, qiuqingbo. I encountered the same requirement as you and have solved it now. Here's my method: I first organized all the sequences to be designed into a single file, then generated every single "DNAWORKS.inp" file for each sequence (by replacing the sequence in the original DNAWORKS.inp file with each of my target sequences). After that, I used a parallel processing script to run all the generated DNAWORKS.inp files simultaneously, obtaining all the design results.