error in step 5

[onmikula]

Hello,

I have two ddrad data sets, each to two different reference genomes (so two sets of fastq files and four genomes in total). With both data sets, I was only able to finish the assembly using one of the reference genomes. When using the other genome, the run ended with this error:

Step 4: Joint estimation of error rate and heterozygosity
[####################] 100% 0:04:01 | inferring [H, E]

Step 5: Consensus base/allele calling
Mean error [0.00082 sd=0.00059]
Mean hetero [0.00173 sd=0.00160]
[####################] 100% 0:00:18 | calculating depths
[####################] 100% 0:00:31 | chunking clusters
[####################] 100% 0:03:15 | consens calling
Exception in step 5: [27:apply] ValueError: too many values to unpack (expected 2)

Encountered an Error.
Message: ValueError: too many values to unpack (expected 2)
Parallel connection closed.

Do you know, what could be the reason?

Ondra

[isaacovercast]

Can you try running step 5 again and including the -d parameter? This will print debug information, so please copy and paste that here.