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sphinx/_build/doctrees/about.doctree

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sphinx/_build/html/.buildinfo

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# Sphinx build info version 1
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# This file hashes the configuration used when building these files. When it is not found, a full rebuild will be done.
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config: ae80a17fba3a79d8ec53a7d5b4a765d6
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config: 14f0da3deab56c51bd3eb0ddaab4ba31
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sphinx/_build/html/_sources/about.rst.txt

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TFpredict is available in two different versions that are merged into one branch. During execution the ``-prokaryote`` argument can be used to choose between the classification of transcription factors from two different domains:
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1. Eukaryotic transcription factors (default)
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2. Prokaryotic σ-factors (-prokaryote option) The algorithm itself is identical. What is different are the training data and weights for both scenarios. So, this distinction is more for convenience to directly provide preconfigured versions of TFpredict for both domains of organisms.
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2. Prokaryotic σ-factors (``-prokaryote`` option): The algorithm itself is identical. What is different are the training data and weights for both scenarios. So, this distinction is more for convenience to directly provide preconfigured versions of TFpredict for both domains of organisms.
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Introduction
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-------------

sphinx/_build/html/_sources/getting_started.rst.txt

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How to get started
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-------------------
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The stand-alone version of TFpredict is equipped with a command-line interface which can be used for the batch processing of multiple protein sequences given in FASTA format. For convenience, TFpredict uses the webservice version of InterproScan. Thus, installing the perl stand-alone version of InterProScan (approx. 40GB) is not required. To support applications, which require the processing of a large number of sequences (e.g., the genome-wide prediction of TFs in a specific organism) TFpredict can alternatively be used with a local installation of InterProScan.
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The stand-alone version of TFpredict is equipped with a command-line interface which can be used for the batch processing of multiple protein sequences given in FASTA format. For convenience, TFpredict uses the webservice version of InterproScan. Thus, installing the perl stand-alone version of InterProScan (approx. 40GB) is not required.
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Installing TFpredict
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---------------------
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Download the JAR file TFpredict from https://github.com/draeger-lab/TFpredict/releases and also the example file test_seq.fasta.
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Download the JAR file TFpredict_1.4 from https://github.com/draeger-lab/TFpredict/releases and also the example file test_seq.fasta.
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You can also clone this repository and build a new snapshot release using the ant script shipped with this project by executing the following command in the ``dist`` folder:
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Next Steps
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----------
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Now that you have TFpredict set up, explore the detailed :ref:`how-to-use`.!
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Now that you have TFpredict set up, explore the detailed :ref:`how-to-use`!
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If you encounter any issues or need further assistance, please refer to other sections of this documentation or feel free to create an issue on our `GitHub repository`_.
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sphinx/_build/html/_sources/use.rst.txt

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.. code-block:: text
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java -jar TFpredict.jar <input_filename> [OPTIONS]
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java -jar TFpredict_1.4.jar <input_filename> [OPTIONS]
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Options
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--------
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- ``-species <organism_name>`` Organism name (e.g., Homo sapiens). See list of `supported organisms`_.
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- ``-tfClassifier <classifier_name>`` Classifier used for TF/non-TF classification possible values: SVM_linear, NaiveBayes, KNN
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- ``-superClassifier <classifier_name>`` Classifier used for superclass prediction possible values: SVM_linear, NaiveBayes, KNN
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- ``-iprscanPath <path_to_iprscan>`` Path to iprscan executable from local InterProScan installation. Only needed if you have a local installation of InterProScan which shall be used by TFpredict.
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- ``-blastPath <path_to_blast>`` Path to "bin" directory containing BLAST executables (e.g., /opt/blast/latest). Only needed if environment variable BLAST_PATH is not set.
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- ``-ignoreCharacteristicDomains`` no classification based on predefined InterPro domains.
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- ``--help`` to display the usage of the script and an overview of the command line options.
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- Organism (see list of `supported organisms`_)
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- Protein sequence
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To run TFpredict on the example input file, use the command:
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To run the newest version of TFpredict on the example input file, use the command:
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.. code-block:: text
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java -jar TFpredict.jar test_seq.fasta
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java -jar TFpredict_1.4.jar test_seq.fasta
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To post-process the results generated by TFpredict with SABINE to predict DNA-motives for transcription factors identified among the input protein sequences, you have to pass two additional arguments to the program. First, the destination to which the output file shall be written has to be specified, and second, the correct species has to be provided. Please ensure that SABINE supports the given species (see list of `supported organisms`_).
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.. code-block:: text
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java -jar TFpredict.jar example_input.fasta -sabineOutfile example_output.txt -species "Homo sapiens"
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A sutitable example.input file
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TFpredict returns an output file, which contains the results of the performed prediction steps in the SABINE input file format.
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If you have a local installation of the tool InterProScan, which shall be used by TFpredict, you have to pass the destination of the main executable of InterProScan as an argument to TFpredict.
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Assuming that InterProScan was installed to the directory ``/opt/iprscan`` you could use the following command:
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.. code-block:: text
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java -jar TFpredict.jar test_seq.fasta -iprscanPath /opt/iprscan/bin/iprscan
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java -jar TFpredict_1.4.jar example_input.fasta -sabineOutfile example_output.txt -species "Homo sapiens"
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For a suitable example_input.fasta file (can be found in the `SABINE`_ GitHub repository) TFpredict returns an output file, which contains the results of the performed prediction steps in the SABINE input file format.
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.. _form_spec:

sphinx/_build/html/about.html

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<p>TFpredict is available in two different versions that are merged into one branch. During execution the <code class="docutils literal notranslate"><span class="pre">-prokaryote</span></code> argument can be used to choose between the classification of transcription factors from two different domains:</p>
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<ol class="arabic simple">
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<li><p>Eukaryotic transcription factors (default)</p></li>
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<li><p>Prokaryotic σ-factors (-prokaryote option) The algorithm itself is identical. What is different are the training data and weights for both scenarios. So, this distinction is more for convenience to directly provide preconfigured versions of TFpredict for both domains of organisms.</p></li>
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<li><p>Prokaryotic σ-factors (<code class="docutils literal notranslate"><span class="pre">-prokaryote</span></code> option): The algorithm itself is identical. What is different are the training data and weights for both scenarios. So, this distinction is more for convenience to directly provide preconfigured versions of TFpredict for both domains of organisms.</p></li>
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<section id="introduction">
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<p>&#169; Copyright 2024, authors.</p>
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<p>&#169; Copyright 2025, Johannes Eichner, Florian Topf, Andreas Dräger, James T. Yurkovich, Michael Römer, Dóra V. Molnár, Michael Gaas.</p>
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sphinx/_build/html/genindex.html

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<p>&#169; Copyright 2024, authors.</p>
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<p>&#169; Copyright 2025, Johannes Eichner, Florian Topf, Andreas Dräger, James T. Yurkovich, Michael Römer, Dóra V. Molnár, Michael Gaas.</p>
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sphinx/_build/html/getting_started.html

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<h2>How to get started<a class="headerlink" href="#how-to-get-started" title="Link to this heading"></a></h2>
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<p>The stand-alone version of TFpredict is equipped with a command-line interface which can be used for the batch processing of multiple protein sequences given in FASTA format. For convenience, TFpredict uses the webservice version of InterproScan. Thus, installing the perl stand-alone version of InterProScan (approx. 40GB) is not required. To support applications, which require the processing of a large number of sequences (e.g., the genome-wide prediction of TFs in a specific organism) TFpredict can alternatively be used with a local installation of InterProScan.</p>
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<p>The stand-alone version of TFpredict is equipped with a command-line interface which can be used for the batch processing of multiple protein sequences given in FASTA format. For convenience, TFpredict uses the webservice version of InterproScan. Thus, installing the perl stand-alone version of InterProScan (approx. 40GB) is not required.</p>
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<section id="installing-tfpredict">
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<h2>Installing TFpredict<a class="headerlink" href="#installing-tfpredict" title="Link to this heading"></a></h2>
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<p>Download the JAR file TFpredict from <a class="reference external" href="https://github.com/draeger-lab/TFpredict/releases">https://github.com/draeger-lab/TFpredict/releases</a> and also the example file test_seq.fasta.</p>
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<p>Download the JAR file TFpredict_1.4 from <a class="reference external" href="https://github.com/draeger-lab/TFpredict/releases">https://github.com/draeger-lab/TFpredict/releases</a> and also the example file test_seq.fasta.</p>
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<p>You can also clone this repository and build a new snapshot release using the ant script shipped with this project by executing the following command in the <code class="docutils literal notranslate"><span class="pre">dist</span></code> folder:</p>
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<div class="highlight-bash notranslate"><div class="highlight"><pre><span></span>ant<span class="w"> </span>-f<span class="w"> </span>tfpredict_build.xml
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<p>Now that you have TFpredict set up, explore the detailed <a class="reference internal" href="use.html#how-to-use"><span class="std std-ref">How to Use TFpredict - The Manual</span></a>.!</p>
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<p>Now that you have TFpredict set up, explore the detailed <a class="reference internal" href="use.html#how-to-use"><span class="std std-ref">How to Use TFpredict - The Manual</span></a>!</p>
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<p>If you encounter any issues or need further assistance, please refer to other sections of this documentation or feel free to create an issue on our <a class="reference external" href="https://github.com/draeger-lab/TFpredict/issues">GitHub repository</a>.</p>
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<p>&#169; Copyright 2024, authors.</p>
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<p>&#169; Copyright 2025, Johannes Eichner, Florian Topf, Andreas Dräger, James T. Yurkovich, Michael Römer, Dóra V. Molnár, Michael Gaas.</p>
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sphinx/_build/html/index.html

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<p>&#169; Copyright 2024, authors.</p>
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<p>&#169; Copyright 2025, Johannes Eichner, Florian Topf, Andreas Dräger, James T. Yurkovich, Michael Römer, Dóra V. Molnár, Michael Gaas.</p>
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sphinx/_build/html/license.html

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<p>&#169; Copyright 2025, Johannes Eichner, Florian Topf, Andreas Dräger, James T. Yurkovich, Michael Römer, Dóra V. Molnár, Michael Gaas.</p>
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sphinx/_build/html/search.html

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<p>&#169; Copyright 2025, Johannes Eichner, Florian Topf, Andreas Dräger, James T. Yurkovich, Michael Römer, Dóra V. Molnár, Michael Gaas.</p>
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