iGEM Distribution 2022 Finalization #25
Description
TODOs
Parts
- Add ApaLI restriction site flankers.
- All of Isaac L's list of parts.
- All preexisting bsub designs
- review isaac G's draft
Description
- Create a README.md file
- Describe components of AllClo
- Describe parts collection
- Attribution and so forth
Package / Submission
- Finalize parts lists and template in Benchling and GSheets
- Add and double-check metadata, ex. authors
- Export to our github fork.
- Submit merge request of our github fork to iGEM-distribution repo.
2022-02-10 Submission Roundup
Links
- Package WIP
- top-level directory: github, google drive
- views subdirectory: github, google drive
- Benchling Drafts
Preliminary list of parts to design and submit for synthesis in the iGEM Distro
- FiveClo/ThreeClo recombination sites
- Ribozyme insulator parts - google spreadsheet
- Part type switching linkers and an expanded level 2 assembly linker set (using BbsI and mBsaI)
- Universal dropout cassette + DOclo linkers
- HpaII and MspI CDS/TU parts
- Partially completed. Full set deferred to subsequent submission.
- Split E. coli selection markers - benchling template files: 8, 9
- Partially completed. Full set deferred to subsequent submission.
- More homology arms for Bsub, Pichia and E. coli
- More constitutive and inducible promoters for Bsub and Pichia
- More Bsub RBSs
- CDSs for auxotrophic selection markers in Bsub and Pichia (+Pichia ku70)
- CDSs for recombinases to go with the FiveClo/ThreeClo recombination sites
- All our existing Bsub wetware part designs, adapted as needed for AllClo
- CDSs for Bsub and Pichia counterselection markers
Stuff from this list that's new
- FiveClo/ThreeClo recombination sites
- More ribozyme insulator parts
- Part type switching linkers and an expanded level 2 assembly linker set
- Universal dropout cassette and DOclo linkers
- HpaII and MspI CDS/TU parts
- More split E. coli selection markers?
- More homology arms for Bsub, Pichia and E. coli
- More constitutive and inducible promoters for Bsub and Pichia
- More Bsub RBSs
- CDSs for auxotrophic selection markers in Bsub and Pichia (+Pichia ku70)
- CDSs for recombinases to go with the FiveClo/ThreeClo recombination sites
- CDSs for Bsub and Pichia counterselection markers
- Homology arms to knock out Pichia proteases PEP4 and PRB1
2022-02-06 Weekly Meet
Action Items
- iGEM Distribution collection discussion.
- Parts / Design Elements
- Add ApaLI restriction site flankers.
- All of Isaac L's list of parts.
- review isaac G's draft, particularly overhangs
- Description
- Create a README.md file referencing the authors of each part
- Ask in github issues about including our own non-automated README for collection.
- Submission workflow:
- Finalize parts lists and template in Benchling and GSheets
- Export to our github fork.
- Submit merge request of our github fork to iGEM-distribution repo.
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