From dd2aa171386dc513b72c478f550ec66ffdd44920 Mon Sep 17 00:00:00 2001
From: Tuomas Borman <tvborm@utu.fi>
Date: Tue, 24 Mar 2026 13:44:53 +0200
Subject: [PATCH] up

---
 inst/pages/example_solutions.qmd     | 94 +++++++++++++++++++---------
 inst/pages/multiassay_ordination.qmd | 13 ++--
 2 files changed, 74 insertions(+), 33 deletions(-)

diff --git a/inst/pages/example_solutions.qmd b/inst/pages/example_solutions.qmd
index a68be250..00ad1ca3 100644
--- a/inst/pages/example_solutions.qmd
+++ b/inst/pages/example_solutions.qmd
@@ -412,26 +412,6 @@ These example solutions are related to exercises in [@sec-mediation].
 #| code-summary: "Show the solution"
 ```
 
-## Network learning & analysis
-
-These example solutions are related to exercises in [@sec-network-learning].
-
-```{r}
-#| label: network1
-#| code-fold: true
-#| code-summary: "Show the solution"
-```
-
-## Network comparison
-
-These example solutions are related to exercises in [@sec-network-comparison].
-
-```{r}
-#| label: network2
-#| code-fold: true
-#| code-summary: "Show the solution"
-```
-
 ## Cross-association
 
 These example solutions are related to exercises in [@sec-cross-correlation].
@@ -440,6 +420,37 @@ These example solutions are related to exercises in [@sec-cross-correlation].
 #| label: cross-corr
 #| code-fold: true
 #| code-summary: "Show the solution"
+
+# Load the example dataset
+library(mia)
+data(HintikkaXOData)
+mae <- HintikkaXOData
+
+# Agglomerate microbiome data
+mae[[1]] <- agglomerateByPrevalence(mae[[1]], rank = "Phylum")
+
+# Apply CLR
+mae[[1]] <- transformAssay(mae[[1]], assay.type = "counts", method = "rclr")
+
+# Apply relative and log10
+mae[[2]] <- transformAssay(mae[[2]], assay.type = "nmr", method = "relabundance")
+mae[[2]] <- transformAssay(mae[[2]], assay.type = "relabundance", method = "log10", pseudocount = TRUE)
+
+# Cross correlates data sets
+res <- getCrossAssociation(
+    mae,
+    experiment1 = 1,
+    experiment2 = 2,
+    assay.type1 = "rclr",
+    assay.type2 = "log10",
+    method = "spearman",
+    mode = "matrix"
+)
+
+# Create a heatmap
+library(ComplexHeatmap)
+p <- Heatmap(res)
+p
 ```
 
 ## Ordination-based multiassay analysis
@@ -451,17 +462,44 @@ These example solutions are related to exercises in
 #| label: mofa
 #| code-fold: true
 #| code-summary: "Show the solution"
-```
 
-## Multi-omics prediction and classification
+# Load the example dataset
+library(mia)
+data(HintikkaXOData)
+mae <- HintikkaXOData
 
-These example solutions are related to exercises in
-[@sec-multi-omics-integration].
+# Select only microbiome and metabolites
+mae <- mae[, , c(1, 2)]
 
-```{r}
-#| label: integratedlearner
-#| code-fold: true
-#| code-summary: "Show the solution"
+# Agglomerate microbiome data
+mae[[1]] <- agglomerateByPrevalence(mae[[1]], rank = "Phylum")
+
+# Apply CLR
+mae[[1]] <- transformAssay(mae[[1]], assay.type = "counts", method = "rclr")
+
+# Apply relative and log10 transformations
+mae[[2]] <- transformAssay(mae[[2]], assay.type = "nmr", method = "relabundance")
+mae[[2]] <- transformAssay(mae[[2]], assay.type = "relabundance", method = "log10", pseudocount = TRUE)
+
+# Scale features
+mae[[1]] <- transformAssay(mae[[1]], assay.type = "rclr", method = "standardize", MARGIN = "rows")
+mae[[2]] <- transformAssay(mae[[2]], assay.type = "log10", method = "standardize", MARGIN = "rows")
+
+# Removing the assays no longer needed
+assays(mae[[1]]) <- assays(mae[[1]])["standardize"]
+assays(mae[[2]]) <- assays(mae[[2]])["standardize"]
+
+# Create a MOFA object
+library(MOFA2)
+model <- create_mofa_from_MultiAssayExperiment(mae)
+
+# Run MOFA
+model <- prepare_mofa(object = model)
+model <- run_mofa(model, use_basilisk = TRUE)
+
+# Visualize variance explained
+p <- plot_variance_explained(model, x = "view", y = "factor")
+p
 ```
 
 ## Machine learning
diff --git a/inst/pages/multiassay_ordination.qmd b/inst/pages/multiassay_ordination.qmd
index 7bfae43e..a80e6b6d 100644
--- a/inst/pages/multiassay_ordination.qmd
+++ b/inst/pages/multiassay_ordination.qmd
@@ -224,17 +224,20 @@ interpret MOFA results.
 4. Apply relative transformation and log10 transformation to other metabolite or
 other omic.
 
-5. Create a MOFA2 object from the `MultiAssayExperiment`.
+5. Standardize each feature (within each dataset) by centering to mean 0 and
+scaling to unit variance so that all variables are on a comparable scale.
 
-6. Run MOFA.
+6. Create a MOFA2 object from the `MultiAssayExperiment`.
 
-7. Visualize the explained variance. How much variance is explained in total,
+7. Run MOFA.
+
+8. Visualize the explained variance. How much variance is explained in total,
 and which omic accounts the most variance?
 
-8. For each omic, visualize the features with the largest weights. Can you
+9. For each omic, visualize the features with the largest weights. Can you
 identify features that are co-varying?
 
-9. If you completed exercises in [@sec-cross-correlation], did you get similar
+10. If you completed exercises in [@sec-cross-correlation], did you get similar
 results? Do the co-varying features also exhibited correlation?
 
 Useful functions: