diff --git a/.chronus/changes/reopen-test-case-encode-bytes-2025-10-28-07-52-39.md b/.chronus/changes/reopen-test-case-encode-bytes-2025-10-28-07-52-39.md
new file mode 100644
index 00000000000..fe0ff54c145
--- /dev/null
+++ b/.chronus/changes/reopen-test-case-encode-bytes-2025-10-28-07-52-39.md
@@ -0,0 +1,7 @@
+---
+changeKind: internal
+packages:
+  - "@typespec/http-client-python"
+---
+
+Reopen test case for base64_url response body encoding
diff --git a/packages/http-client-python/generator/test/generic_mock_api_tests/asynctests/test_encode_bytes_async.py b/packages/http-client-python/generator/test/generic_mock_api_tests/asynctests/test_encode_bytes_async.py
index 52612763836..93f8f62c68d 100644
--- a/packages/http-client-python/generator/test/generic_mock_api_tests/asynctests/test_encode_bytes_async.py
+++ b/packages/http-client-python/generator/test/generic_mock_api_tests/asynctests/test_encode_bytes_async.py
@@ -124,5 +124,4 @@ async def test_response_body(client: BytesClient, png_data: bytes):
     assert expected == await client.response_body.base64()
     assert b"".join([d async for d in (await client.response_body.octet_stream())]) == png_data
     assert b"".join([d async for d in (await client.response_body.custom_content_type())]) == png_data
-    # will reopen after TCGC release a fix version for https://github.com/Azure/typespec-azure/pull/2411
-    # assert expected == await client.response_body.base64_url()
+    assert expected == await client.response_body.base64_url()
diff --git a/packages/http-client-python/generator/test/generic_mock_api_tests/test_encode_bytes.py b/packages/http-client-python/generator/test/generic_mock_api_tests/test_encode_bytes.py
index 666f6d14fba..615d4f050e0 100644
--- a/packages/http-client-python/generator/test/generic_mock_api_tests/test_encode_bytes.py
+++ b/packages/http-client-python/generator/test/generic_mock_api_tests/test_encode_bytes.py
@@ -119,5 +119,4 @@ def test_response_body(client: BytesClient, png_data: bytes):
     assert expected == client.response_body.base64()
     assert b"".join(client.response_body.octet_stream()) == png_data
     assert b"".join(client.response_body.custom_content_type()) == png_data
-    # will reopen after TCGC release a fix version for https://github.com/Azure/typespec-azure/pull/2411
-    # assert expected == client.response_body.base64_url()
+    assert expected == client.response_body.base64_url()