TridiagEigen: eigen decomposition failed

[GaoLabXDU]

Dear Jingsi,

Thanks very much for your user-friendly package!

However, I encountered one issue when applying FIRM. I was trying to apply FIRM for integrating three 10x datasets, which is the 6th benchmark test in [1] and it contains three batches of cell line data. I used the log-scaled data with 2000 HVGs as input for FIRM. The following error appeared.

"Error in FIRM_res_all(Dataset1[hvg, ], FindClusters1, Dataset2[hvg, ], :
TridiagEigen: eigen decomposition failed"

Could you please be so kind as to suggest some solution to it?

Thanks in advance and looking forward to your reply!

Best regards,
Han Xu

1. Tran,H.T.N., Ang,K.S., Chevrier,M., Zhang,X., Lee,N.Y.S., Goh,M. and Chen,J. (2020) A benchmark of batch-effect correction methods for single-cell RNA sequencing data. Genome Biology, 21, 12.