From d1bca6599ce6ffb57a41f92e391f518d86164b69 Mon Sep 17 00:00:00 2001 From: "Oliver M. Crook" Date: Tue, 15 Feb 2022 10:34:56 +0000 Subject: [PATCH 01/12] add secA data --- inst/extdata/Project_2_SecA_Cluster_Data.csv | 31856 +++++++++++++++++ 1 file changed, 31856 insertions(+) create mode 100644 inst/extdata/Project_2_SecA_Cluster_Data.csv diff --git a/inst/extdata/Project_2_SecA_Cluster_Data.csv b/inst/extdata/Project_2_SecA_Cluster_Data.csv new file mode 100644 index 0000000..8ff7204 --- /dev/null +++ b/inst/extdata/Project_2_SecA_Cluster_Data.csv @@ -0,0 +1,31856 @@ +Protein,Start,End,Sequence,Modification,Fragment,MaxUptake,MHP,State,Exposure,File,z,RT,Inten,Center +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,0,291018_SecA_REF_5,1,3.693435,710541,475.530986 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,0,291018_SecA_REF_4,1,4.077553,13038,476.83212 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,0,291018_SecA_REF_3,1,3.719365,819312,475.442147 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,0,291018_SecA_REF_2,1,3.712732,559986,475.482948 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,0,291018_SecA_REF_1,1,3.677982,527423,475.514738 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,0.25,011118_SecA_15sec_1,1,3.724814,506213,475.903634 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,0.25,011118_SecA_15sec_2,1,3.703513,982228,475.693106 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,0.25,011118_SecA_15sec_3,1,3.694405,1141330,475.814398 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,1,011118_SecA_1min_1,1,3.752395,565455,475.973345 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,1,011118_SecA_1min_2,1,3.706973,849022,475.987154 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,1,011118_SecA_1min_3,1,3.725339,850994,475.985709 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,5,011118_SecA_5min_1,1,3.714814,1074754,476.4025 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,5,011118_SecA_5min_2,1,3.716668,776860,476.492942 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,5,011118_SecA_5min_3,1,3.71624,1082966,476.384766 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,30.000002,011118_SecA_30min_1,1,3.70575,981129,476.625177 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,30.000002,011118_SecA_30min_2,1,3.736798,1217900,476.595259 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA,30.000002,011118_SecA_30min_3,1,3.689852,1154137,476.612493 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,0,011118_SecA_YEG_ref_2,1,3.707809,1314080,475.399478 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,0,011118_SecA_YEG_ref_3,1,3.706421,704807,475.460023 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,0,011118_SecA_YEG_ref_1,1,3.707084,841178,475.468299 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,0.25,011118_SecA_YEG_15sec_1,1,3.730407,317676,476.197469 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,0.25,011118_SecA_YEG_15sec_2,1,3.7212,498056,476.194254 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,0.25,011118_SecA_YEG_15sec_3,1,3.719965,690872,476.247659 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,1,011118_SecA_YEG_1min_1,1,3.736633,755542,476.342928 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,1,011118_SecA_YEG_1min_2,1,3.709018,590690,476.398671 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,1,011118_SecA_YEG_1min_3,1,3.707352,630547,476.353412 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,5,011118_SecA_YEG_5min_1,1,3.70705,702473,476.512487 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,5,011118_SecA_YEG_5min_2,1,3.730424,831847,476.49479 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,5,011118_SecA_YEG_5min_3,1,3.727571,735195,476.520994 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,30.000002,011118_SecA_YEG_30min_1,1,3.733754,760113,476.659711 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,30.000002,011118_SecA_YEG_30min_2,1,3.702166,795166,476.665269 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG,30.000002,011118_SecA_YEG_30min_3,1,3.688552,810943,476.647147 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,1,3.707809,1314080,475.399478 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,1,3.706421,704807,475.460023 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,1,3.707084,841178,475.468299 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,1,3.754649,607294,476.34161 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,1,3.713854,613437,476.271144 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,1,3.735569,541044,476.339691 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,1,3.731859,672088,476.414739 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,1,3.692494,544016,476.368808 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,1,3.753933,684505,476.368473 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,1,3.731119,859544,476.435443 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,1,3.73563,869230,476.476991 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,1,3.72006,793143,476.452582 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,1,3.710438,810382,476.710123 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,1,3.710048,936015,476.794049 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,1,3.704036,758611,476.6879 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,1,3.707809,1314080,475.399478 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,1,3.706421,704807,475.460023 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,1,3.707084,841178,475.468299 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,1,3.779104,539911,476.275393 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,1,3.7343,544236,476.230158 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,1,3.679502,41069,477.763481 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,1,3.733531,583680,476.28578 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,1,3.76275,550014,476.23336 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,1,3.755357,632436,476.280864 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,1,3.679909,431399,476.349868 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,1,3.746149,554296,476.359252 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,1,3.748881,563309,476.394827 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,1,3.717216,841193,476.55783 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,1,3.6863,623590,476.834038 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,1,3.703925,497217,476.627883 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,0,291018_SecA_REF_5,1,3.693435,710541,475.530986 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,0,291018_SecA_REF_4,1,4.077553,13038,476.83212 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,0,291018_SecA_REF_3,1,3.719365,819312,475.442147 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,0,291018_SecA_REF_2,1,3.712732,559986,475.482948 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,0,291018_SecA_REF_1,1,3.677982,527423,475.514738 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,0.25,311018_SecA_ADP_15sec_4,1,3.724323,716581,475.985488 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,0.25,311018_SecA_ADP_15sec_3,1,3.745965,928430,475.916438 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,0.25,301018_SecA_ADP_15sec_2,1,3.700718,735623,476.105744 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,1,311018_SecA_ADP_1min_3,1,3.720631,707381,476.221226 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,1,311018_SecA_ADP_1min_2,1,3.747091,817265,476.281758 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,1,311018_SecA_ADP_1min_1,1,3.717054,527051,476.234854 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,5,311018_SecA_ADP_5min_3,1,3.743076,1206673,476.434652 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,5,311018_SecA_ADP_5min_2,1,3.68254,760853,476.438083 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,5,311018_SecA_ADP_5min_1,1,3.629596,739185,476.660233 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,30.000002,311018_SecA_ADP_30min_3,1,3.688583,883583,476.692292 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,30.000002,311018_SecA_ADP_30min_2,1,3.746727,1164853,476.678011 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_ADP,30.000002,311018_SecA_ADP_30min_1,1,3.763724,992428,476.678283 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,0,291018_SecA_REF_5,1,3.693435,710541,475.530986 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,0,291018_SecA_REF_4,1,4.077553,13038,476.83212 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,0,291018_SecA_REF_3,1,3.719365,819312,475.442147 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,0,291018_SecA_REF_2,1,3.712732,559986,475.482948 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,0,291018_SecA_REF_1,1,3.677982,527423,475.514738 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_3,1,3.708591,418302,475.992902 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_2,1,3.727111,541522,475.978003 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_1,1,3.700563,383342,475.982004 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_3,1,3.734997,481651,476.24606 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_2,1,3.748595,516016,476.257914 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_1,1,3.734498,440531,476.23919 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_3,1,3.693548,543672,476.706788 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_2,1,3.754125,719066,476.376346 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_1,1,3.735281,699908,476.444896 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_3,1,3.748059,672117,476.675413 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_2,1,3.808881,31369,476.710464 +RANDOM,4,8,LGGTQ,,,4,475.2511,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_1,1,3.770224,820063,476.647418 +SecA,1,5,MLIKL,,,4,617.4055,SecA,0,291018_SecA_REF_5,1,5.802883,476646,617.746656 +SecA,1,5,MLIKL,,,4,617.4055,SecA,0,291018_SecA_REF_4,1,5.819075,326242,617.769744 +SecA,1,5,MLIKL,,,4,617.4055,SecA,0,291018_SecA_REF_3,1,5.791974,423822,617.830448 +SecA,1,5,MLIKL,,,4,617.4055,SecA,0,291018_SecA_REF_2,1,5.81223,290265,617.75729 +SecA,1,5,MLIKL,,,4,617.4055,SecA,0,291018_SecA_REF_1,1,5.800816,282233,617.736313 +SecA,1,5,MLIKL,,,4,617.4055,SecA,0.25,011118_SecA_15sec_1,1,5.775852,216159,619.944382 +SecA,1,5,MLIKL,,,4,617.4055,SecA,0.25,011118_SecA_15sec_2,1,5.771716,299244,620.05974 +SecA,1,5,MLIKL,,,4,617.4055,SecA,0.25,011118_SecA_15sec_3,1,5.770833,330762,620.057343 +SecA,1,5,MLIKL,,,4,617.4055,SecA,1,011118_SecA_1min_1,1,5.79493,207374,620.065519 +SecA,1,5,MLIKL,,,4,617.4055,SecA,1,011118_SecA_1min_2,1,5.787162,324674,620.127939 +SecA,1,5,MLIKL,,,4,617.4055,SecA,1,011118_SecA_1min_3,1,5.784251,372040,620.127545 +SecA,1,5,MLIKL,,,4,617.4055,SecA,5,011118_SecA_5min_1,1,5.790558,402015,620.016107 +SecA,1,5,MLIKL,,,4,617.4055,SecA,5,011118_SecA_5min_2,1,5.774486,449683,620.019491 +SecA,1,5,MLIKL,,,4,617.4055,SecA,5,011118_SecA_5min_3,1,5.786316,351122,620.012069 +SecA,1,5,MLIKL,,,4,617.4055,SecA,30.000002,011118_SecA_30min_1,1,5.792298,398322,620.062114 +SecA,1,5,MLIKL,,,4,617.4055,SecA,30.000002,011118_SecA_30min_2,1,5.777629,405565,620.031358 +SecA,1,5,MLIKL,,,4,617.4055,SecA,30.000002,011118_SecA_30min_3,1,5.775825,420473,620.028614 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+SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_ADP,5,311018_SecA_ADP_5min_3,2,3.973591,1197001,600.24762 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_ADP,5,311018_SecA_ADP_5min_2,2,3.941812,491730,600.195834 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_ADP,5,311018_SecA_ADP_5min_1,2,3.972889,121163,600.156126 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_ADP,30.000002,311018_SecA_ADP_30min_3,2,3.956695,861399,600.468547 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_ADP,30.000002,311018_SecA_ADP_30min_2,2,3.981897,969747,600.381391 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_ADP,30.000002,311018_SecA_ADP_30min_1,2,3.995508,978491,600.486049 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,0,291018_SecA_REF_5,2,3.96457,751319,598.684704 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,0,291018_SecA_REF_4,2,3.968842,689903,598.653728 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,0,291018_SecA_REF_3,2,3.974887,879145,598.680764 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,0,291018_SecA_REF_2,2,3.964747,489494,598.792173 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,0,291018_SecA_REF_1,2,3.948852,842331,598.64485 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_3,2,3.986698,522116,599.652279 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_2,2,3.99153,641871,599.630425 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_1,2,3.965133,486171,599.592437 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_3,2,4.029103,584957,599.826411 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_2,2,4.03674,482185,599.878222 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_1,2,4.021518,568738,599.904374 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_3,2,3.980116,414808,600.157397 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_2,2,4.035061,718832,600.186723 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_1,2,4.023124,685299,600.241044 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_3,2,4.039515,745910,600.364752 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_2,2,4.04077,44472,600.376924 +SecA,119,130,NALTGKGVHVVT,,,11,1195.6793,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_1,2,4.038147,673179,600.437948 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecA,0,291018_SecA_REF_5,2,4.152241,13970692,762.802322 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecA,0,291018_SecA_REF_4,2,4.162406,13304618,762.784762 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecA,0,291018_SecA_REF_3,2,4.141991,16086658,762.818789 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecA,0,291018_SecA_REF_2,2,4.139948,11724013,762.792114 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+SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecA,30.000002,011118_SecA_30min_1,2,4.156715,14177422,765.185162 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecA,30.000002,011118_SecA_30min_3,2,4.143741,13375464,765.238653 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,0,291018_SecA_SecYEG_REF_3,2,4.199183,111482,762.800597 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,0,291018_SecA_SecYEG_REF_2,2,4.188774,264286,762.781913 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,0,291018_SecA_YEG_REF_1,2,4.161528,222523,762.775272 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,0,011118_SecA_YEG_ref_2,2,4.1602,13641769,762.792535 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,0,011118_SecA_YEG_ref_3,2,4.138829,9872361,762.81092 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,0,011118_SecA_YEG_ref_1,2,4.161934,14594821,762.824145 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,0.25,011118_SecA_YEG_15sec_1,2,4.16207,3547802,763.937172 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,0.25,011118_SecA_YEG_15sec_2,2,4.147896,7430416,763.930829 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,0.25,011118_SecA_YEG_15sec_3,2,4.151266,9409999,763.884895 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,1,011118_SecA_YEG_1min_1,2,4.15995,8723563,764.135781 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,1,011118_SecA_YEG_1min_2,2,4.151605,7155073,764.181056 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,1,011118_SecA_YEG_1min_3,2,4.145761,7690616,764.146384 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,5,011118_SecA_YEG_5min_1,2,4.160646,10081874,764.759896 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,5,011118_SecA_YEG_5min_2,2,4.154953,13179718,764.744104 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,5,011118_SecA_YEG_5min_3,2,4.14498,8802361,764.847932 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG,30.000002,011118_SecA_YEG_30min_1,2,4.154357,9968909,765.274994 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+SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,2,4.164919,6578148,764.053262 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,2,4.156362,9565256,764.035326 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,2,4.171855,8333442,764.378362 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,2,4.163221,7865773,764.227129 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,2,4.161738,7008538,764.252885 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,2,4.163911,13334862,764.603511 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,2,4.150245,11610638,764.594382 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,2,4.147005,11887986,764.662803 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,2,4.15114,11616588,764.940128 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,2,4.14975,11589049,764.964592 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,2,4.146942,11830439,764.919666 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,2,4.199183,111482,762.800597 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,2,4.188774,264286,762.781913 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,0,291018_SecA_YEG_REF_1,2,4.161528,222523,762.775272 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,2,4.1602,13641769,762.792535 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,2,4.138829,9872361,762.81092 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,2,4.161934,14594821,762.824145 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,2,4.202523,10949587,764.059627 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,2,4.20393,7866978,764.006834 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,2,4.193304,10547592,764.028303 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,2,4.193494,9722603,764.286201 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,2,4.208609,7684083,764.27136 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,2,4.204215,9918004,764.277035 +SecA,119,133,NALTGKGVHVVTVND,,,14,1523.8176,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,2,4.166543,10644477,764.591989 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+SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG,30.000002,011118_SecA_YEG_30min_3,1,4.085565,685007,813.058649 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,1,4.103668,833220,811.889055 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,1,4.100082,694512,811.884225 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,1,4.115937,638253,811.82827 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,1,4.135318,457644,812.358111 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,1,4.112514,455193,812.346861 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,1,4.125892,437050,812.334029 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,1,4.131061,514805,812.449872 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,1,4.109515,487798,812.355207 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,1,4.142628,446830,812.354803 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,1,4.116735,762556,812.535023 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,1,4.127296,822232,812.495582 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,1,4.109939,630258,812.521839 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,1,4.103621,723959,812.684694 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,1,4.117506,799471,812.673248 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,1,4.108148,790843,812.583941 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,1,4.103668,833220,811.889055 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,1,4.100082,694512,811.884225 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,1,4.115937,638253,811.82827 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,1,4.168933,504064,812.269916 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,1,4.159429,385830,812.265791 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,1,4.169647,535485,812.228103 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,1,4.159719,506976,812.26284 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,1,4.169946,431789,812.289558 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,1,4.17145,526571,812.319175 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,1,4.123034,512878,812.418812 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,1,4.142501,484399,812.455958 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,1,4.148709,436759,812.473063 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,1,4.142929,689249,812.484558 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,1,4.128643,593202,812.495338 +SecA,186,191,YLRDNM,,,5,811.3767,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,1,4.135618,476216,812.510304 +SecA,186,191,YLRDNM,,,5,811.3767,SecA_ADP,0,291018_SecA_REF_5,1,4.106792,572918,811.866446 +SecA,186,191,YLRDNM,,,5,811.3767,SecA_ADP,0,291018_SecA_REF_4,1,4.127125,449403,811.832731 +SecA,186,191,YLRDNM,,,5,811.3767,SecA_ADP,0,291018_SecA_REF_3,1,4.11322,698398,811.86678 +SecA,186,191,YLRDNM,,,5,811.3767,SecA_ADP,0,291018_SecA_REF_2,1,4.108644,453562,811.82534 +SecA,186,191,YLRDNM,,,5,811.3767,SecA_ADP,0,291018_SecA_REF_1,1,4.098403,367982,811.833147 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+SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecA,5,011118_SecA_5min_2,3,4.753112,436298,528.931251 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecA,5,011118_SecA_5min_3,3,4.764492,358877,528.854648 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecA,30.000002,011118_SecA_30min_1,3,4.754278,338523,529.458495 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecA,30.000002,011118_SecA_30min_2,3,4.748733,351037,529.345757 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecA,30.000002,011118_SecA_30min_3,3,4.748457,372413,529.402608 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,0,291018_SecA_SecYEG_REF_3,3,4.870533,42400,528.179698 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,0,291018_SecA_SecYEG_REF_2,3,4.887537,87975,528.193253 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,0,291018_SecA_YEG_REF_1,3,4.833639,82269,528.195586 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,0,011118_SecA_YEG_ref_2,3,4.754065,330246,528.199805 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,0,011118_SecA_YEG_ref_3,3,4.753128,437696,528.120843 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,0,011118_SecA_YEG_ref_1,3,4.770624,294875,528.216552 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,0.25,011118_SecA_YEG_15sec_1,3,4.803417,225999,528.711282 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,0.25,011118_SecA_YEG_15sec_2,3,4.775578,253697,528.644716 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,0.25,011118_SecA_YEG_15sec_3,3,4.763775,346033,528.638358 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,1,011118_SecA_YEG_1min_1,3,4.764754,313312,528.648295 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,1,011118_SecA_YEG_1min_2,3,4.766205,244932,528.621337 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,1,011118_SecA_YEG_1min_3,3,4.773544,237474,528.642178 +SecA,216,230,IDEARTPLIISGPAE,,,12,1581.8483,SecAYEG,5,011118_SecA_YEG_5min_1,3,4.760521,243388,528.87491 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+SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,3,4.369968,3449579,461.173129 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,3,4.376845,2974849,461.172579 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,3,4.43381,1312605,461.587733 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,3,4.399586,2277176,461.588857 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,3,4.415365,1414708,461.547501 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,3,4.429033,2042832,461.772205 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,3,4.415019,1625801,461.711288 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,3,4.450671,1446734,461.755137 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,3,4.399559,2165805,462.08542 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,3,4.415596,2424333,461.995422 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,3,4.392529,2141615,461.995769 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,3,4.386353,1792003,462.393583 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,3,4.387839,2331315,462.374262 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,3,4.406342,2029519,462.373802 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,3,4.415414,121933,461.208767 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,3,4.415771,206197,461.206943 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,0,291018_SecA_YEG_REF_1,3,4.398074,204304,461.20601 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,3,4.377658,4754085,461.161958 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,3,4.369968,3449579,461.173129 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,3,4.376845,2974849,461.172579 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,3,4.67904,1422091,461.56824 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,3,4.688795,1351231,461.521115 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,3,4.655553,1472386,461.503377 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,3,4.68778,1917376,461.768924 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,3,4.71547,1626980,461.669898 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,3,4.672277,1511758,461.675581 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,3,4.575223,1844382,461.939886 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,3,4.54172,1496778,461.936924 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,3,4.541356,1993219,461.854113 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,3,4.58599,2632406,462.042027 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,3,4.562955,2345912,462.109515 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,3,4.585127,2054188,462.097664 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,0,291018_SecA_REF_5,3,4.338351,2525083,461.137881 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,0,291018_SecA_REF_4,3,4.388455,3068521,461.159734 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,0,291018_SecA_REF_3,3,4.35432,2915884,461.164939 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,0,291018_SecA_REF_2,3,4.365047,760570,461.197505 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,0,291018_SecA_REF_1,3,4.353793,816228,461.177152 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,0.25,311018_SecA_ADP_15sec_4,3,4.36962,2393849,461.514303 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,0.25,311018_SecA_ADP_15sec_3,3,4.34558,2460080,461.492501 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,0.25,301018_SecA_ADP_15sec_2,3,4.561791,3102131,461.493407 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,1,311018_SecA_ADP_1min_3,3,4.385077,2246192,461.58213 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,1,311018_SecA_ADP_1min_2,3,4.381608,2124798,461.601426 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,1,311018_SecA_ADP_1min_1,3,4.408501,1604796,461.654367 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,5,311018_SecA_ADP_5min_3,3,4.411788,2632633,461.947326 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,5,311018_SecA_ADP_5min_1,3,4.423628,2411044,461.926888 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,30.000002,311018_SecA_ADP_30min_3,3,4.408227,2645504,462.284957 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,30.000002,311018_SecA_ADP_30min_2,3,4.404228,2560260,462.302139 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_ADP,30.000002,311018_SecA_ADP_30min_1,3,4.442992,1604809,462.205041 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,0,291018_SecA_REF_5,3,4.338351,2525083,461.137881 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,0,291018_SecA_REF_4,3,4.388455,3068521,461.159734 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,0,291018_SecA_REF_3,3,4.35432,2915884,461.164939 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,0,291018_SecA_REF_2,3,4.365047,760570,461.197505 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,0,291018_SecA_REF_1,3,4.353793,816228,461.177152 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_3,3,4.525879,1020650,461.459592 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_2,3,4.435577,1292424,461.489858 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_1,3,4.355179,1027674,461.52197 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_3,3,4.644792,1013231,461.58831 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_1,3,4.574278,1128306,461.567804 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_3,3,4.636366,1433674,461.919566 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_2,3,4.670845,1436658,461.865736 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_3,3,4.70442,1318126,462.086554 +SecA,236,246,YKRVNKIIPHL,,,9,1380.8474,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_2,3,4.65015,985325,462.370346 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,0,291018_SecA_REF_5,2,4.389372,256703,1019.509132 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,0,291018_SecA_REF_4,2,4.394649,191810,1019.558946 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,0,291018_SecA_REF_3,2,4.390126,254897,1019.525547 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,0,291018_SecA_REF_2,2,4.383793,119378,1019.513567 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,0,291018_SecA_REF_1,2,4.374694,140965,1019.465679 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,0.25,011118_SecA_15sec_2,2,4.369462,233764,1023.119919 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,0.25,011118_SecA_15sec_3,2,4.372376,260185,1023.137734 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,1,011118_SecA_1min_1,2,4.394266,96934,1023.10014 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,1,011118_SecA_1min_2,2,4.386978,202414,1023.217542 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,1,011118_SecA_1min_3,2,4.379093,354535,1023.233607 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,5,011118_SecA_5min_2,2,4.371898,446775,1023.294065 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,5,011118_SecA_5min_3,2,4.378037,315904,1023.040317 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,30.000002,011118_SecA_30min_1,2,4.38981,337417,1023.269115 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,30.000002,011118_SecA_30min_2,2,4.375811,339980,1023.308511 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA,30.000002,011118_SecA_30min_3,2,4.371421,424808,1023.265091 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG,0,011118_SecA_YEG_ref_2,2,4.374369,637942,1019.531123 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG,0,011118_SecA_YEG_ref_3,2,4.37743,558021,1019.490074 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG,0,011118_SecA_YEG_ref_1,2,4.387181,532830,1019.527225 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG,0.25,011118_SecA_YEG_15sec_2,2,4.385725,162272,1023.053107 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG,0.25,011118_SecA_YEG_15sec_3,2,4.384993,340971,1023.039062 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG,1,011118_SecA_YEG_1min_1,2,4.377681,278303,1023.29894 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG,1,011118_SecA_YEG_1min_2,2,4.374243,209661,1023.087716 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG,1,011118_SecA_YEG_1min_3,2,4.378518,182129,1023.146415 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG,5,011118_SecA_YEG_5min_1,2,4.389222,309507,1023.080932 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG,5,011118_SecA_YEG_5min_3,2,4.364157,418282,1023.173138 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG,30.000002,011118_SecA_YEG_30min_2,2,4.366894,359906,1023.253363 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG,30.000002,011118_SecA_YEG_30min_3,2,4.353191,443506,1023.281991 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,2,4.374369,637942,1019.531123 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,2,4.37743,558021,1019.490074 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,2,4.387181,532830,1019.527225 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,2,4.398924,163751,1022.926581 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,2,4.385798,221508,1022.93777 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,2,4.395241,142380,1022.810693 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,2,4.400084,187886,1023.001755 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,2,4.388807,191713,1023.070248 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,2,4.407225,129718,1022.844901 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,2,4.381321,363893,1022.961119 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,2,4.37098,304013,1022.946353 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,2,4.376308,416840,1023.175574 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,2,4.382998,422003,1023.101745 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,2,4.385488,432939,1023.022758 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,2,4.374369,637942,1019.531123 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,2,4.37743,558021,1019.490074 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,2,4.387181,532830,1019.527225 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,2,4.443213,98747,1022.272505 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,2,4.443233,119584,1022.356529 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,2,4.44156,157492,1022.689425 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,2,4.444422,117914,1022.51781 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,2,4.44103,130464,1022.735394 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,2,4.406703,290636,1022.739139 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,2,4.412968,296007,1022.667784 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,2,4.41123,185909,1022.557295 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,2,4.421249,301838,1022.921544 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,2,4.414102,364571,1022.854755 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,2,4.41926,245917,1022.926268 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,0,291018_SecA_REF_5,2,4.389372,256703,1019.509132 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,0,291018_SecA_REF_4,2,4.394649,191810,1019.558946 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,0,291018_SecA_REF_3,2,4.390126,254897,1019.525547 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,0,291018_SecA_REF_2,2,4.383793,119378,1019.513567 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,0,291018_SecA_REF_1,2,4.374694,140965,1019.465679 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,0.25,311018_SecA_ADP_15sec_4,2,4.394512,221287,1023.126504 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,0.25,311018_SecA_ADP_15sec_3,2,4.388853,302147,1023.092103 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,0.25,301018_SecA_ADP_15sec_2,2,4.414345,182851,1022.972643 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,1,311018_SecA_ADP_1min_3,2,4.39727,229561,1023.204585 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,1,311018_SecA_ADP_1min_2,2,4.395377,201691,1023.193161 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,1,311018_SecA_ADP_1min_1,2,4.401291,88498,1023.120818 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,5,311018_SecA_ADP_5min_3,2,4.385212,455181,1023.082375 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,5,311018_SecA_ADP_5min_1,2,4.378226,391479,1023.023551 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,30.000002,311018_SecA_ADP_30min_3,2,4.384171,281409,1023.131067 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,30.000002,311018_SecA_ADP_30min_2,2,4.387907,336732,1023.180762 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_ADP,30.000002,311018_SecA_ADP_30min_1,2,4.402823,337866,1023.156146 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,0,291018_SecA_REF_5,2,4.389372,256703,1019.509132 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,0,291018_SecA_REF_4,2,4.394649,191810,1019.558946 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,0,291018_SecA_REF_3,2,4.390126,254897,1019.525547 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,0,291018_SecA_REF_2,2,4.383793,119378,1019.513567 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,0,291018_SecA_REF_1,2,4.374694,140965,1019.465679 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_3,2,4.420696,83427,1022.675374 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_2,2,4.397123,109722,1022.972821 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_3,2,4.442441,71146,1022.993489 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_1,2,4.434272,87432,1022.912765 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_3,2,4.434472,181411,1022.678568 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_2,2,4.431144,109920,1022.730476 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_1,2,4.430801,103603,1022.692297 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_3,2,4.4551,116263,1022.730795 +SecA,247,263,IRQEKEDSETFQGEGHF,,,16,2036.9308,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_2,2,4.429742,137632,1022.738378 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,0,291018_SecA_REF_5,2,4.57136,6699397,411.359945 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,0,291018_SecA_REF_4,2,4.565542,3600001,411.411395 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,0,291018_SecA_REF_3,2,4.575492,5121806,411.39867 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,0,291018_SecA_REF_2,2,4.603289,3658999,411.409576 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,0,291018_SecA_REF_1,2,4.551535,3995464,411.352793 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,0.25,011118_SecA_15sec_1,2,4.562611,2853511,412.552764 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,0.25,011118_SecA_15sec_2,2,4.549308,5926786,412.544018 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,0.25,011118_SecA_15sec_3,2,4.563784,6223262,412.517187 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,1,011118_SecA_1min_1,2,4.574727,3695776,412.572708 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,1,011118_SecA_1min_2,2,4.558547,5046052,412.622779 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,1,011118_SecA_1min_3,2,4.571672,5448118,412.583308 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,5,011118_SecA_5min_1,2,4.574769,5521920,412.667539 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,5,011118_SecA_5min_2,2,4.56081,4659770,412.675004 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,5,011118_SecA_5min_3,2,4.571448,5317437,412.669922 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,30.000002,011118_SecA_30min_1,2,4.573916,6064988,412.693375 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,30.000002,011118_SecA_30min_2,2,4.548488,5754038,412.700017 +SecA,257,263,FQGEGHF,,,6,821.3577,SecA,30.000002,011118_SecA_30min_3,2,4.5602,5944463,412.688314 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,0,291018_SecA_SecYEG_REF_3,2,4.597616,83078,411.383521 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,0,291018_SecA_SecYEG_REF_2,2,4.595182,200793,411.375606 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,0,291018_SecA_YEG_REF_1,2,4.571685,155685,411.423369 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,0,011118_SecA_YEG_ref_2,2,4.551126,4833603,411.361165 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,0,011118_SecA_YEG_ref_3,2,4.546837,4530002,411.357353 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,0,011118_SecA_YEG_ref_1,2,4.573306,5101760,411.35366 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,0.25,011118_SecA_YEG_15sec_1,2,4.595758,1868000,412.429941 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,0.25,011118_SecA_YEG_15sec_2,2,4.572764,2823407,412.46775 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,0.25,011118_SecA_YEG_15sec_3,2,4.566129,4283025,412.534927 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,1,011118_SecA_YEG_1min_1,2,4.566203,3214097,412.631617 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,1,011118_SecA_YEG_1min_2,2,4.562069,4167703,412.557251 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,1,011118_SecA_YEG_1min_3,2,4.565342,4405121,412.49455 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,5,011118_SecA_YEG_5min_1,2,4.574471,3921104,412.569087 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,5,011118_SecA_YEG_5min_2,2,4.568237,3513996,412.676338 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,5,011118_SecA_YEG_5min_3,2,4.550536,3482032,412.689775 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,30.000002,011118_SecA_YEG_30min_1,2,4.554122,3323006,412.66578 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,30.000002,011118_SecA_YEG_30min_2,2,4.555171,3314163,412.703461 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG,30.000002,011118_SecA_YEG_30min_3,2,4.553069,3306048,412.698608 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_3,2,4.597616,83078,411.383521 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,2,4.595182,200793,411.375606 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG_ADP,0,291018_SecA_YEG_REF_1,2,4.571685,155685,411.423369 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,2,4.551126,4833603,411.361165 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,2,4.546837,4530002,411.357353 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,2,4.573306,5101760,411.35366 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,2,4.583939,3472295,412.494585 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,2,4.559565,3651823,412.485717 +SecA,257,263,FQGEGHF,,,6,821.3577,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,2,4.572045,2644441,412.466551 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+SecA,410,428,VVVPTNRPMIRKDLPDLVY,,,15,2225.2475,SecA,0,291018_SecA_REF_1,4,5.272324,19770472,557.435871 +SecA,410,428,VVVPTNRPMIRKDLPDLVY,,,15,2225.2475,SecA,0.25,011118_SecA_15sec_1,4,5.262916,15905300,558.047976 +SecA,410,428,VVVPTNRPMIRKDLPDLVY,,,15,2225.2475,SecA,0.25,011118_SecA_15sec_2,4,5.251011,28427122,558.045867 +SecA,410,428,VVVPTNRPMIRKDLPDLVY,,,15,2225.2475,SecA,0.25,011118_SecA_15sec_3,4,5.255076,30807617,558.048116 +SecA,410,428,VVVPTNRPMIRKDLPDLVY,,,15,2225.2475,SecA,1,011118_SecA_1min_1,4,5.271262,19093790,558.237256 +SecA,410,428,VVVPTNRPMIRKDLPDLVY,,,15,2225.2475,SecA,1,011118_SecA_1min_2,4,5.281323,28378361,558.241554 +SecA,410,428,VVVPTNRPMIRKDLPDLVY,,,15,2225.2475,SecA,1,011118_SecA_1min_3,4,5.265688,31207643,558.230452 +SecA,410,428,VVVPTNRPMIRKDLPDLVY,,,15,2225.2475,SecA,5,011118_SecA_5min_1,4,5.27406,34588213,558.7183 +SecA,410,428,VVVPTNRPMIRKDLPDLVY,,,15,2225.2475,SecA,5,011118_SecA_5min_2,4,5.27293,36774981,558.724344 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+SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_ADP,5,311018_SecA_ADP_5min_3,3,4.923026,5223475,590.687701 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_ADP,5,311018_SecA_ADP_5min_2,3,4.944662,5658374,590.647374 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_ADP,5,311018_SecA_ADP_5min_1,3,4.92776,5059376,590.635969 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_ADP,30.000002,311018_SecA_ADP_30min_3,3,4.929098,5716702,591.175964 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_ADP,30.000002,311018_SecA_ADP_30min_2,3,4.924731,5487430,591.178678 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_ADP,30.000002,311018_SecA_ADP_30min_1,3,4.938951,6899569,591.19005 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,0,291018_SecA_REF_5,3,4.923039,9453315,589.303785 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,0,291018_SecA_REF_4,3,4.948312,5359536,589.337456 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,0,291018_SecA_REF_3,3,4.922108,8250160,589.304563 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,0,291018_SecA_REF_2,3,4.949208,4287398,589.336646 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,0,291018_SecA_REF_1,3,4.926115,5784732,589.326851 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_3,3,4.969367,5395509,590.177337 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_2,3,4.9554,5620569,590.200566 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_1,3,4.934196,3338315,590.235257 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_3,3,4.995564,3913184,590.444092 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_2,3,4.997343,3939958,590.554246 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_1,3,4.987436,4089441,590.472825 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_3,3,4.975861,5831504,591.019095 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_2,3,4.993404,5120376,590.945459 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_1,3,5.004862,5728698,590.918911 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_3,3,5.008334,5943249,591.339582 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_2,3,4.995231,4032252,591.350177 +SecA,412,426,VPTNRPMIRKDLPDL,,,11,1764.9789,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_1,3,4.993827,5048013,591.35387 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,0,291018_SecA_REF_5,1,3.669694,98361,826.78552 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,0,291018_SecA_REF_4,1,3.683037,65208,826.772595 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,0,291018_SecA_REF_3,1,3.721615,138150,826.751853 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,0,291018_SecA_REF_2,1,3.725225,92042,826.76439 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,0,291018_SecA_REF_1,1,3.660583,40601,826.668843 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,0.25,011118_SecA_15sec_1,1,3.737984,77465,828.494491 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,0.25,011118_SecA_15sec_2,1,3.711691,133410,828.618257 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,0.25,011118_SecA_15sec_3,1,3.670698,108531,828.553798 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,1,011118_SecA_1min_1,1,3.765019,93332,828.54357 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,1,011118_SecA_1min_2,1,3.687475,112123,828.615645 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,1,011118_SecA_1min_3,1,3.742801,180856,828.683625 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,5,011118_SecA_5min_1,1,3.714005,158131,828.976854 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,5,011118_SecA_5min_2,1,3.729975,193181,828.902407 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,5,011118_SecA_5min_3,1,3.723239,151825,828.865657 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,30.000002,011118_SecA_30min_1,1,3.67601,99152,829.269731 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,30.000002,011118_SecA_30min_2,1,3.755868,170147,829.27935 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA,30.000002,011118_SecA_30min_3,1,3.656574,107560,829.094256 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,0,011118_SecA_YEG_ref_2,1,3.68235,192790,826.775114 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,0,011118_SecA_YEG_ref_3,1,3.710716,199234,826.748622 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,0,011118_SecA_YEG_ref_1,1,3.698443,153391,826.740516 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,0.25,011118_SecA_YEG_15sec_1,1,3.733655,60903,828.373238 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,0.25,011118_SecA_YEG_15sec_2,1,3.71572,98964,828.592233 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,0.25,011118_SecA_YEG_15sec_3,1,3.713117,148234,828.527555 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,1,011118_SecA_YEG_1min_1,1,3.751578,116808,828.729959 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,1,011118_SecA_YEG_1min_2,1,3.697336,112813,828.660888 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,1,011118_SecA_YEG_1min_3,1,3.679853,69066,828.472998 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,5,011118_SecA_YEG_5min_1,1,3.711393,148342,828.960837 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,5,011118_SecA_YEG_5min_3,1,3.744129,167764,829.01088 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,30.000002,011118_SecA_YEG_30min_1,1,3.752673,149973,829.381188 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,30.000002,011118_SecA_YEG_30min_2,1,3.695193,131472,829.306028 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG,30.000002,011118_SecA_YEG_30min_3,1,3.658217,134268,829.270528 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,1,3.68235,192790,826.775114 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,1,3.710716,199234,826.748622 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+SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,1,3.737682,140789,829.051312 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,1,3.698652,137328,829.344012 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,1,3.669945,79445,829.233083 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,1,3.672753,82007,829.225235 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,1,3.68235,192790,826.775114 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,1,3.710716,199234,826.748622 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,1,3.698443,153391,826.740516 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,1,3.784893,86468,828.530185 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,1,3.708156,62598,828.395411 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,1,3.784015,88142,828.543703 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,1,3.707263,77631,828.752876 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,1,3.762008,79539,828.701156 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,1,3.752139,100163,828.747124 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,1,3.75437,132443,828.96037 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,1,3.768718,117640,828.967109 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,1,3.690066,94026,829.234962 +SecA,426,432,LVYMTEA,,,6,826.4015,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,1,3.658107,18827,829.176201 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,0,291018_SecA_REF_5,1,3.669694,98361,826.78552 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,0,291018_SecA_REF_4,1,3.683037,65208,826.772595 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,0,291018_SecA_REF_3,1,3.721615,138150,826.751853 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,0,291018_SecA_REF_2,1,3.725225,92042,826.76439 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,0,291018_SecA_REF_1,1,3.660583,40601,826.668843 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,0.25,311018_SecA_ADP_15sec_4,1,3.732821,116581,828.677439 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,0.25,311018_SecA_ADP_15sec_3,1,3.756365,182667,828.667459 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,0.25,301018_SecA_ADP_15sec_2,1,3.656081,41317,828.597418 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,1,311018_SecA_ADP_1min_3,1,3.69625,86286,828.733955 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,1,311018_SecA_ADP_1min_2,1,3.759201,134220,828.871127 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,1,311018_SecA_ADP_1min_1,1,3.68899,49119,828.723344 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,5,311018_SecA_ADP_5min_3,1,3.753656,150130,829.087721 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,30.000002,311018_SecA_ADP_30min_3,1,3.652294,51655,829.153904 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,30.000002,311018_SecA_ADP_30min_2,1,3.764703,170047,829.389126 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_ADP,30.000002,311018_SecA_ADP_30min_1,1,3.781514,177572,829.410975 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,0,291018_SecA_REF_5,1,3.669694,98361,826.78552 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,0,291018_SecA_REF_4,1,3.683037,65208,826.772595 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,0,291018_SecA_REF_3,1,3.721615,138150,826.751853 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,0,291018_SecA_REF_2,1,3.725225,92042,826.76439 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,0,291018_SecA_REF_1,1,3.660583,40601,826.668843 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_3,1,3.664972,24656,828.518958 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_2,1,3.731409,87643,828.559695 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_1,1,3.683817,25463,828.383755 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_3,1,3.721475,62838,828.707595 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_2,1,3.758901,58756,828.750976 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_1,1,3.718504,44815,828.764419 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_3,1,3.637647,9457,829.220989 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_2,1,3.761475,87504,829.022641 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_1,1,3.745503,90538,828.988005 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_3,1,3.742036,96332,829.159769 +SecA,426,432,LVYMTEA,,,6,826.4015,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_1,1,3.778731,101423,829.251083 +SecA,427,431,VYMTE,,,4,642.2803,SecA,0,291018_SecA_REF_5,1,4.20385,2405357,642.622394 +SecA,427,431,VYMTE,,,4,642.2803,SecA,0,291018_SecA_REF_4,1,4.211689,2659773,642.576732 +SecA,427,431,VYMTE,,,4,642.2803,SecA,0,291018_SecA_REF_3,1,4.204825,3363782,642.543565 +SecA,427,431,VYMTE,,,4,642.2803,SecA,0,291018_SecA_REF_2,1,4.198411,2410386,642.615927 +SecA,427,431,VYMTE,,,4,642.2803,SecA,0,291018_SecA_REF_1,1,4.192008,1820189,642.611846 +SecA,427,431,VYMTE,,,4,642.2803,SecA,0.25,011118_SecA_15sec_1,1,4.214506,1323189,643.14909 +SecA,427,431,VYMTE,,,4,642.2803,SecA,0.25,011118_SecA_15sec_2,1,4.20665,2653897,643.093655 +SecA,427,431,VYMTE,,,4,642.2803,SecA,0.25,011118_SecA_15sec_3,1,4.198664,2817479,643.065128 +SecA,427,431,VYMTE,,,4,642.2803,SecA,1,011118_SecA_1min_1,1,4.22984,1539188,643.50534 +SecA,427,431,VYMTE,,,4,642.2803,SecA,1,011118_SecA_1min_2,1,4.210781,2306968,643.471477 +SecA,427,431,VYMTE,,,4,642.2803,SecA,1,011118_SecA_1min_3,1,4.212195,3536119,643.389946 +SecA,427,431,VYMTE,,,4,642.2803,SecA,5,011118_SecA_5min_1,1,4.218915,3156221,644.071892 +SecA,427,431,VYMTE,,,4,642.2803,SecA,5,011118_SecA_5min_2,1,4.203354,3539109,644.13478 +SecA,427,431,VYMTE,,,4,642.2803,SecA,5,011118_SecA_5min_3,1,4.207628,3469101,644.081456 +SecA,427,431,VYMTE,,,4,642.2803,SecA,30.000002,011118_SecA_30min_1,1,4.209874,3102818,644.277284 +SecA,427,431,VYMTE,,,4,642.2803,SecA,30.000002,011118_SecA_30min_2,1,4.209848,3156049,644.264921 +SecA,427,431,VYMTE,,,4,642.2803,SecA,30.000002,011118_SecA_30min_3,1,4.193745,2894923,644.179453 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,0,291018_SecA_SecYEG_REF_3,1,4.241873,50222,642.476549 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,0,291018_SecA_SecYEG_REF_2,1,4.245249,133841,642.628467 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,0,291018_SecA_YEG_REF_1,1,4.207438,95831,642.58086 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,0,011118_SecA_YEG_ref_2,1,4.205735,4800253,642.496367 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,0,011118_SecA_YEG_ref_3,1,4.213429,3452097,642.506727 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,0,011118_SecA_YEG_ref_1,1,4.219053,3362414,642.548374 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,0.25,011118_SecA_YEG_15sec_1,1,4.224091,1011482,643.28238 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,0.25,011118_SecA_YEG_15sec_2,1,4.209051,1375088,643.341441 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,0.25,011118_SecA_YEG_15sec_3,1,4.220584,2688308,643.166803 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,1,011118_SecA_YEG_1min_1,1,4.223097,3015467,643.646425 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,1,011118_SecA_YEG_1min_2,1,4.208153,1768819,643.712874 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,1,011118_SecA_YEG_1min_3,1,4.209016,1666220,643.732938 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,5,011118_SecA_YEG_5min_1,1,4.217902,2030310,644.24098 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,5,011118_SecA_YEG_5min_2,1,4.217002,3319547,644.121646 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,5,011118_SecA_YEG_5min_3,1,4.215527,2456433,644.172006 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,30.000002,011118_SecA_YEG_30min_1,1,4.21884,2783136,644.237755 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,30.000002,011118_SecA_YEG_30min_2,1,4.202146,2729524,644.294608 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG,30.000002,011118_SecA_YEG_30min_3,1,4.190034,3189234,644.404563 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_3,1,4.241873,50222,642.476549 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,1,4.245249,133841,642.628467 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,0,291018_SecA_YEG_REF_1,1,4.207438,95831,642.58086 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,1,4.205735,4800253,642.496367 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,1,4.213429,3452097,642.506727 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,1,4.219053,3362414,642.548374 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,1,4.229085,1658635,643.491321 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,1,4.210025,2365136,643.444199 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,1,4.212938,2380012,643.449185 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,1,4.220963,2191270,643.939099 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,1,4.209447,1988730,643.912402 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,1,4.22977,1536605,643.993727 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,1,4.215526,2973623,644.056808 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,1,4.219974,2880272,644.169526 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,1,4.202723,2942064,644.072994 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,1,4.20528,2616049,644.268866 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,1,4.209827,3131176,644.153474 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,1,4.203786,2775282,644.198658 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,1,4.241873,50222,642.476549 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,1,4.245249,133841,642.628467 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,0,291018_SecA_YEG_REF_1,1,4.207438,95831,642.58086 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,1,4.205735,4800253,642.496367 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,1,4.213429,3452097,642.506727 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,1,4.219053,3362414,642.548374 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,1,4.244007,1717996,643.352108 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,1,4.234416,1293211,643.246849 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,1,4.236037,2260782,643.174545 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,1,4.224408,2480451,643.734637 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,1,4.244871,1524977,643.760368 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,1,4.242425,2302141,643.758635 +SecA,427,431,VYMTE,,,4,642.2803,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,1,4.206001,2390680,644.219187 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+SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_ADP,30.000002,311018_SecA_ADP_30min_2,2,4.263373,1085418,803.003887 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_ADP,30.000002,311018_SecA_ADP_30min_1,2,4.271165,1088078,802.982592 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,0,291018_SecA_REF_5,2,4.277807,844917,800.212997 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,0,291018_SecA_REF_4,2,4.27553,539285,800.235907 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,0,291018_SecA_REF_3,2,4.259701,727868,800.23119 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,0,291018_SecA_REF_2,2,4.257016,516893,800.293472 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,0,291018_SecA_REF_1,2,4.250308,522554,800.273217 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_3,2,4.273357,570057,801.036166 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_2,2,4.267416,681848,801.097979 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_1,2,4.266443,557705,801.036071 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_3,2,4.29143,692782,801.462994 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_2,2,4.291913,590030,801.454676 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_1,2,4.298406,605660,801.487899 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_3,2,4.282769,942490,802.212286 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_2,2,4.295714,800747,802.206918 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_1,2,4.288675,733169,802.171299 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_3,2,4.301015,962712,803.006352 +SecA,648,660,YDDVANDQRRAIY,,,12,1598.7558,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_2,2,4.255871,840955,802.875413 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,0,291018_SecA_REF_5,2,4.041051,479521,718.791393 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,0,291018_SecA_REF_4,2,4.060514,348713,718.753477 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,0,291018_SecA_REF_3,2,4.051662,435650,718.733159 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,0,291018_SecA_REF_2,2,4.04492,370832,718.762776 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,0,291018_SecA_REF_1,2,4.033163,324373,718.734084 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,0.25,011118_SecA_15sec_1,2,4.054292,264578,719.5187 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,0.25,011118_SecA_15sec_2,2,4.043445,393921,719.558088 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,0.25,011118_SecA_15sec_3,2,4.040254,322133,719.554639 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,1,011118_SecA_1min_1,2,4.063367,216789,719.767708 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,1,011118_SecA_1min_2,2,4.055988,376604,719.852667 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,1,011118_SecA_1min_3,2,4.050351,418664,719.880948 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,5,011118_SecA_5min_1,2,4.050711,420551,720.810391 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,5,011118_SecA_5min_2,2,4.041066,368999,720.900366 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,5,011118_SecA_5min_3,2,4.044091,392791,720.824911 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,30.000002,011118_SecA_30min_1,2,4.045366,427453,721.589168 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,30.000002,011118_SecA_30min_2,2,4.046444,435257,721.533052 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA,30.000002,011118_SecA_30min_3,2,4.038434,470509,721.606702 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,0,291018_SecA_SecYEG_REF_2,2,4.293302,5207,718.605886 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,0,011118_SecA_YEG_ref_2,2,4.050324,694156,718.658588 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,0,011118_SecA_YEG_ref_3,2,4.04812,383965,718.663757 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,0,011118_SecA_YEG_ref_1,2,4.053453,435579,718.708232 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,0.25,011118_SecA_YEG_15sec_2,2,4.056782,195254,719.485498 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,0.25,011118_SecA_YEG_15sec_3,2,4.048939,286163,719.590022 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,1,011118_SecA_YEG_1min_1,2,4.060979,251433,719.788994 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,1,011118_SecA_YEG_1min_2,2,4.048758,261026,719.897436 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,1,011118_SecA_YEG_1min_3,2,4.047159,245907,719.902789 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,5,011118_SecA_YEG_5min_1,2,4.052061,268036,720.753715 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,5,011118_SecA_YEG_5min_2,2,4.057913,383459,720.702621 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,5,011118_SecA_YEG_5min_3,2,4.04674,324029,720.726401 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,30.000002,011118_SecA_YEG_30min_1,2,4.051279,372325,721.39548 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,30.000002,011118_SecA_YEG_30min_2,2,4.035065,343942,721.431835 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG,30.000002,011118_SecA_YEG_30min_3,2,4.024416,379210,721.488451 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,2,4.293302,5207,718.605886 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,2,4.050324,694156,718.658588 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,2,4.04812,383965,718.663757 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,2,4.053453,435579,718.708232 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,2,4.073178,320411,719.732935 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,2,4.061497,291286,719.690481 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,2,4.044876,343226,720.12585 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,2,4.077437,301292,720.140029 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,2,4.055041,528078,720.940589 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,2,4.055671,543025,720.90223 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,2,4.044509,412274,720.855292 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,2,4.040686,425142,721.647543 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,2,4.044934,402844,721.613915 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,2,4.293302,5207,718.605886 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,2,4.050324,694156,718.658588 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,2,4.04812,383965,718.663757 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,2,4.053453,435579,718.708232 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,2,4.1007,295831,719.517275 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,2,4.099502,350528,719.400265 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,2,4.095994,273024,719.771274 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,2,4.101654,361770,719.829549 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,2,4.049514,261644,720.288547 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,2,4.074017,245570,720.190048 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,2,4.064054,293601,720.790034 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,0,291018_SecA_REF_5,2,4.041051,479521,718.791393 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,0,291018_SecA_REF_4,2,4.060514,348713,718.753477 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,0,291018_SecA_REF_3,2,4.051662,435650,718.733159 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,0,291018_SecA_REF_2,2,4.04492,370832,718.762776 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,0,291018_SecA_REF_1,2,4.033163,324373,718.734084 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,0.25,311018_SecA_ADP_15sec_4,2,4.059631,500685,719.677075 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,0.25,311018_SecA_ADP_15sec_3,2,4.06068,488240,719.687904 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,0.25,301018_SecA_ADP_15sec_2,2,4.06632,442572,719.655295 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,1,311018_SecA_ADP_1min_2,2,4.064196,515803,720.256842 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,1,311018_SecA_ADP_1min_1,2,4.058833,321686,720.194389 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,5,311018_SecA_ADP_5min_3,2,4.053452,671379,721.08198 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,5,311018_SecA_ADP_5min_2,2,4.040391,485201,721.039448 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,5,311018_SecA_ADP_5min_1,2,4.013164,495341,720.98933 +SecA,649,660,DDVANDQRRAIY,,,11,1435.6924,SecA_ADP,30.000002,311018_SecA_ADP_30min_3,2,4.037964,551072,721.679877 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+SecA,716,722,LDLPIAE,,,5,770.4294,SecA,0.25,011118_SecA_15sec_3,1,5.656688,952504,771.175883 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA,1,011118_SecA_1min_1,1,5.678167,602900,771.42756 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA,1,011118_SecA_1min_2,1,5.669994,860449,771.545395 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA,1,011118_SecA_1min_3,1,5.672224,1083126,771.524307 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA,5,011118_SecA_5min_1,1,5.675123,1038224,772.286243 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA,5,011118_SecA_5min_2,1,5.659863,1172066,772.39509 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA,5,011118_SecA_5min_3,1,5.669147,1017518,772.279548 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA,30.000002,011118_SecA_30min_1,1,5.674978,1036695,773.178705 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA,30.000002,011118_SecA_30min_2,1,5.663151,918008,773.12111 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA,30.000002,011118_SecA_30min_3,1,5.659844,1151145,773.13553 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,0,291018_SecA_SecYEG_REF_3,1,5.693363,24728,770.664077 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,0,291018_SecA_SecYEG_REF_2,1,5.698534,74045,770.807999 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,0,291018_SecA_YEG_REF_1,1,5.690786,53010,770.681589 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,0,011118_SecA_YEG_ref_2,1,5.679646,863319,770.829011 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,0,011118_SecA_YEG_ref_3,1,5.670484,632387,770.800706 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,0,011118_SecA_YEG_ref_1,1,5.685891,733336,770.833564 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,0.25,011118_SecA_YEG_15sec_1,1,5.6848,348570,771.235014 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,0.25,011118_SecA_YEG_15sec_2,1,5.678073,465687,771.223147 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,0.25,011118_SecA_YEG_15sec_3,1,5.679287,606687,771.266929 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,1,011118_SecA_YEG_1min_1,1,5.681374,561874,771.591706 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,1,011118_SecA_YEG_1min_2,1,5.664134,482275,771.606779 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,1,011118_SecA_YEG_1min_3,1,5.67711,493329,771.528763 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,5,011118_SecA_YEG_5min_1,1,5.673936,488213,772.286394 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,5,011118_SecA_YEG_5min_2,1,5.672739,623953,772.385973 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,5,011118_SecA_YEG_5min_3,1,5.665635,659504,772.431571 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,30.000002,011118_SecA_YEG_30min_1,1,5.665202,608390,773.171072 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,30.000002,011118_SecA_YEG_30min_2,1,5.662907,591026,773.171451 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG,30.000002,011118_SecA_YEG_30min_3,1,5.657181,651845,773.156798 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_3,1,5.693363,24728,770.664077 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,1,5.698534,74045,770.807999 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,0,291018_SecA_YEG_REF_1,1,5.690786,53010,770.681589 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,1,5.679646,863319,770.829011 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,1,5.670484,632387,770.800706 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,1,5.685891,733336,770.833564 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,1,5.676271,640306,771.394302 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,1,5.66758,624838,771.403645 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,1,5.676032,618770,771.403212 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,1,5.681904,647405,771.935898 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,1,5.674369,540317,771.930396 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,1,5.687528,547764,771.950033 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,1,5.670763,673299,772.865807 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,1,5.673232,862978,772.748463 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,1,5.660174,779609,772.791789 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,1,5.66282,794475,773.330461 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,1,5.671689,764174,773.388724 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,1,5.672081,741813,773.228029 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,1,5.693363,24728,770.664077 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,1,5.698534,74045,770.807999 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,0,291018_SecA_YEG_REF_1,1,5.690786,53010,770.681589 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,1,5.679646,863319,770.829011 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,1,5.670484,632387,770.800706 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,1,5.685891,733336,770.833564 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,1,5.694641,567845,771.270852 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,1,5.68819,521954,771.214856 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,1,5.690866,584135,771.229966 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,1,5.697705,644990,771.835293 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,1,5.69488,479222,771.791475 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,1,5.69206,582787,771.82704 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,1,5.689948,695657,772.662876 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,1,5.688503,584796,772.584286 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,1,5.692854,552752,772.593396 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,1,5.68352,817958,773.076599 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,1,5.684513,636204,773.050795 +SecA,716,722,LDLPIAE,,,5,770.4294,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,1,5.684869,680109,773.045124 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,0,291018_SecA_REF_5,1,5.671212,1139132,770.808509 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,0,291018_SecA_REF_4,1,5.692057,772059,770.867634 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,0,291018_SecA_REF_3,1,5.668724,946255,770.900487 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,0,291018_SecA_REF_2,1,5.686686,750844,770.832778 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,0,291018_SecA_REF_1,1,5.679165,580787,770.919662 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,0.25,311018_SecA_ADP_15sec_4,1,5.681638,988009,771.435388 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,0.25,311018_SecA_ADP_15sec_3,1,5.669475,1119397,771.338635 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,0.25,301018_SecA_ADP_15sec_2,1,5.685275,933591,771.41142 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,1,311018_SecA_ADP_1min_3,1,5.673398,935733,771.894694 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,1,311018_SecA_ADP_1min_2,1,5.679175,969475,771.959078 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,1,311018_SecA_ADP_1min_1,1,5.687364,744683,771.857698 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,5,311018_SecA_ADP_5min_3,1,5.668497,1301014,772.867222 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,5,311018_SecA_ADP_5min_2,1,5.678235,1179048,772.960758 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,5,311018_SecA_ADP_5min_1,1,5.664518,1329288,772.871319 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,30.000002,311018_SecA_ADP_30min_3,1,5.669687,1046456,773.27524 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,30.000002,311018_SecA_ADP_30min_2,1,5.674928,1216262,773.290121 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_ADP,30.000002,311018_SecA_ADP_30min_1,1,5.682412,1084151,773.332269 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_AMPPNP,0,291018_SecA_REF_5,1,5.671212,1139132,770.808509 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_AMPPNP,0,291018_SecA_REF_4,1,5.692057,772059,770.867634 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_AMPPNP,0,291018_SecA_REF_3,1,5.668724,946255,770.900487 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_AMPPNP,0,291018_SecA_REF_2,1,5.686686,750844,770.832778 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_AMPPNP,0,291018_SecA_REF_1,1,5.679165,580787,770.919662 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_3,1,5.683113,588151,771.391308 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_2,1,5.68187,702134,771.389864 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_1,1,5.688468,480966,771.325947 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_3,1,5.696151,602930,771.933491 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_2,1,5.700766,598657,771.938794 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_1,1,5.701816,645860,771.94763 +SecA,716,722,LDLPIAE,,,5,770.4294,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_3,1,5.686777,962651,772.780479 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+SecG,34,42,FGAGASATL,,,8,794.4043,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,1,5.046437,281591,798.426524 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,0,291018_SecA_SecYEG_REF_3,1,4.075836,461204,647.704515 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,0,291018_SecA_SecYEG_REF_2,1,4.086336,400956,647.627437 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,0,291018_SecA_YEG_REF_1,1,4.04914,357997,647.62509 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,0,011118_SecA_YEG_ref_2,1,4.052089,1869525,647.527301 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,0,011118_SecA_YEG_ref_3,1,4.047073,1501733,647.520307 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,0,011118_SecA_YEG_ref_1,1,4.064198,1563937,647.519774 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,0.25,011118_SecA_YEG_15sec_1,1,4.063179,435044,651.163127 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,0.25,011118_SecA_YEG_15sec_2,1,4.055344,560522,651.289364 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,1,011118_SecA_YEG_1min_1,1,4.067013,826690,651.00899 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,1,011118_SecA_YEG_1min_2,1,4.048105,704893,650.854207 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,1,011118_SecA_YEG_1min_3,1,4.05226,684691,650.8326 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,5,011118_SecA_YEG_5min_1,1,4.055119,856350,650.886597 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,5,011118_SecA_YEG_5min_2,1,4.0614,1155174,650.870526 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,5,011118_SecA_YEG_5min_3,1,4.054917,958890,650.851975 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,30.000002,011118_SecA_YEG_30min_1,1,4.06016,998310,650.785695 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,30.000002,011118_SecA_YEG_30min_2,1,4.042984,940097,650.829201 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG,30.000002,011118_SecA_YEG_30min_3,1,4.03108,1060702,650.695876 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_3,1,4.075836,461204,647.704515 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,1,4.086336,400956,647.627437 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,0,291018_SecA_YEG_REF_1,1,4.04914,357997,647.62509 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,1,4.052089,1869525,647.527301 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,1,4.047073,1501733,647.520307 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,1,4.064198,1563937,647.519774 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,1,4.070265,674367,650.780492 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,1,4.044787,605001,651.062384 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,1,4.065145,755123,650.7352 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,1,4.041191,688174,650.872353 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,1,4.074423,631550,650.720772 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,1,4.054708,892324,650.668439 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,1,4.063169,1009568,650.713014 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,1,4.047863,823786,650.749815 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,1,4.043972,919439,650.695819 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,1,4.052098,934066,650.584293 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,1,4.04907,989046,650.654468 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,1,4.075836,461204,647.704515 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,1,4.086336,400956,647.627437 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,0,291018_SecA_YEG_REF_1,1,4.04914,357997,647.62509 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,1,4.052089,1869525,647.527301 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,1,4.047073,1501733,647.520307 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,1,4.064198,1563937,647.519774 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,1,4.083505,792897,650.922081 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,1,4.069111,668497,650.947178 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,1,4.075395,848823,650.680163 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,1,4.077806,852809,650.567165 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,1,4.081186,728071,650.797155 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,1,4.045793,1005436,650.386428 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,1,4.070377,893353,650.537009 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,1,4.076248,852458,650.528093 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,1,4.062023,1153999,650.655497 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,1,4.048784,1153501,650.625466 +SecG,35,42,GAGASATL,,,7,647.3359,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,1,4.058203,912638,650.560055 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,0,291018_SecA_SecYEG_REF_3,1,5.726813,18100,538.485117 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,0,291018_SecA_SecYEG_REF_2,1,5.676578,18253,538.438473 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,0,011118_SecA_YEG_ref_2,1,5.650687,311509,538.552335 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,0,011118_SecA_YEG_ref_3,1,5.643332,281129,538.575431 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,0.25,011118_SecA_YEG_15sec_1,1,5.665207,245190,538.73424 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,0.25,011118_SecA_YEG_15sec_2,1,5.652181,292573,538.709265 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,0.25,011118_SecA_YEG_15sec_3,1,5.656617,274423,538.704624 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,1,011118_SecA_YEG_1min_1,1,5.658935,283531,538.797087 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,1,011118_SecA_YEG_1min_2,1,5.640451,338099,538.728796 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,1,011118_SecA_YEG_1min_3,1,5.651906,307655,538.767491 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,5,011118_SecA_YEG_5min_1,1,5.647962,270110,539.119945 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,5,011118_SecA_YEG_5min_2,1,5.649323,263964,539.120426 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,5,011118_SecA_YEG_5min_3,1,5.648693,245103,539.116321 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,30.000002,011118_SecA_YEG_30min_1,1,5.641888,271286,539.513571 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,30.000002,011118_SecA_YEG_30min_2,1,5.638007,255218,539.597223 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG,30.000002,011118_SecA_YEG_30min_3,1,5.635539,247540,539.641949 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_3,1,5.726813,18100,538.485117 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,1,5.676578,18253,538.438473 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,1,5.650687,311509,538.552335 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,1,5.643332,281129,538.575431 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,1,5.659551,323574,538.713954 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,1,5.649716,286816,538.764014 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,1,5.659122,324456,538.763402 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,1,5.656774,285092,538.793024 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,1,5.67288,275778,538.765098 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,1,5.64915,339459,539.103704 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,1,5.65086,376015,539.064577 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,1,5.648163,344633,539.018629 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,1,5.644495,334468,539.747041 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,1,5.655879,346605,539.678385 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,1,5.654938,301313,539.662447 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,1,5.726813,18100,538.485117 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,1,5.676578,18253,538.438473 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,1,5.650687,311509,538.552335 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,1,5.643332,281129,538.575431 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,1,5.680055,314907,538.646494 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,1,5.676214,308314,538.657808 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,1,5.675887,395941,538.644891 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,1,5.680928,350322,538.67361 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,1,5.678419,291617,538.72859 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,1,5.678416,321836,538.707158 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,1,5.671977,297576,538.872211 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,1,5.669249,252896,538.841556 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,1,5.673315,283684,538.839478 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,1,5.6695,350921,539.045868 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,1,5.669524,259592,539.07208 +SecG,39,43,SATLF,,,4,538.2871,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,1,5.670276,294824,539.018451 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,0,291018_SecA_SecYEG_REF_3,1,4.949912,363372,859.800954 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,0,291018_SecA_SecYEG_REF_2,1,4.967542,272977,859.717285 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,0,291018_SecA_YEG_REF_1,1,4.928376,272418,859.724886 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,0,011118_SecA_YEG_ref_2,1,4.922603,2350354,859.655123 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,0,011118_SecA_YEG_ref_3,1,4.919473,2470027,859.649764 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,0,011118_SecA_YEG_ref_1,1,4.935852,1798394,859.724503 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,0.25,011118_SecA_YEG_15sec_1,1,4.943401,478335,861.36781 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,0.25,011118_SecA_YEG_15sec_2,1,4.930763,668062,861.457793 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,0.25,011118_SecA_YEG_15sec_3,1,4.935511,1026538,861.34604 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,1,011118_SecA_YEG_1min_1,1,4.934277,877660,861.387797 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,1,011118_SecA_YEG_1min_2,1,4.922693,34755,865.628957 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,5,011118_SecA_YEG_5min_1,1,4.932296,1358090,861.277739 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,5,011118_SecA_YEG_5min_3,1,4.923217,1460022,861.3439 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG,30.000002,011118_SecA_YEG_30min_3,1,4.913047,1530138,861.250215 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_3,1,4.949912,363372,859.800954 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,1,4.967542,272977,859.717285 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,0,291018_SecA_YEG_REF_1,1,4.928376,272418,859.724886 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,1,4.922603,2350354,859.655123 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,1,4.919473,2470027,859.649764 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,1,4.935852,1798394,859.724503 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,1,4.940537,676394,861.236264 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,1,4.927845,620143,861.321161 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,1,4.938438,531279,861.581264 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,1,4.939587,798736,861.113135 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,1,4.934421,802799,861.230775 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,1,4.944785,728906,861.237505 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,1,4.932436,1084863,861.194189 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,1,4.934554,1360686,861.239663 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,1,4.928623,1185300,861.175863 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,1,4.923836,1194886,861.166931 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,1,4.93137,1238371,861.169193 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,1,4.931896,1209664,861.22546 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,1,4.949912,363372,859.800954 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,1,4.967542,272977,859.717285 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_AMPPNP,0,291018_SecA_YEG_REF_1,1,4.928376,272418,859.724886 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,1,4.922603,2350354,859.655123 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,1,4.919473,2470027,859.649764 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,1,4.935852,1798394,859.724503 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,1,4.942913,584505,861.386686 +SecG,43,51,FGSSGSGNF,,,8,859.3581,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,1,4.943519,423102,861.499005 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+SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_3,2,4.94575,13863429,440.53147 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,2,4.970732,9136894,440.556598 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0,291018_SecA_YEG_REF_1,1,4.950417,378215,879.996664 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0,291018_SecA_YEG_REF_1,2,4.932112,5739316,440.470852 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,1,4.934233,1870345,879.890304 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,2,4.931005,21007347,440.550389 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,1,4.929972,1913997,879.853054 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,2,4.929458,20315994,440.544387 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,1,4.945493,1410996,879.960594 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,2,4.939144,16216768,440.537373 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,1,4.958745,712573,880.962937 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,2,4.95244,13501157,440.969808 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,1,4.944272,710817,880.987122 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,2,4.950776,10760237,440.997705 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,1,4.952788,591082,881.017572 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,2,4.949419,7723329,440.953644 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,1,4.951806,822197,881.254051 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,2,4.940365,11892567,441.107684 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,1,4.953906,789175,881.238354 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,2,4.9519,10874633,441.084972 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,1,4.960957,700877,881.219429 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,2,4.94852,9565036,441.110233 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,1,4.948529,1304161,881.657687 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,2,4.949872,16416067,441.355451 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,1,4.947262,1530928,881.706274 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,2,4.943534,21498298,441.39615 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,1,4.937861,1260476,882.311696 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,2,4.936598,14163443,441.708914 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,1,4.941892,1245452,882.334794 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,2,4.940772,14463019,441.730952 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,1,4.940269,1384065,882.355282 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,2,4.93067,18239162,441.734211 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,1,4.97393,437782,880.116509 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,2,4.94575,13863429,440.53147 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,2,4.970732,9136894,440.556598 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0,291018_SecA_YEG_REF_1,1,4.950417,378215,879.996664 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0,291018_SecA_YEG_REF_1,2,4.932112,5739316,440.470852 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,1,4.934233,1870345,879.890304 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,2,4.931005,21007347,440.550389 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,1,4.929972,1913997,879.853054 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,2,4.929458,20315994,440.544387 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,1,4.945493,1410996,879.960594 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,2,4.939144,16216768,440.537373 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,1,4.972863,762995,880.937757 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,2,4.955024,12494369,440.982089 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,1,4.982784,690913,880.923202 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,2,4.970087,12835406,440.970137 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,1,4.981918,748690,881.001602 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,2,4.975587,11538103,440.984191 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,1,4.985509,992493,881.247264 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,2,4.972672,17120422,441.146787 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,1,4.9809,842564,881.233958 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,2,4.974971,15202288,441.138285 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,1,4.985834,994426,881.247154 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,2,4.988127,16532593,441.128237 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,1,4.970996,1489361,881.711044 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,2,4.956685,17495421,441.311332 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,1,4.961643,1411371,881.65307 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,2,4.965981,22726615,441.321639 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,1,4.968838,1372293,881.676659 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,2,4.973127,18057691,441.350636 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,1,4.966928,1412793,882.438922 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,2,4.953409,20858785,441.780309 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,1,4.969475,1610261,882.406372 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,2,4.965737,19269024,441.783711 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,1,4.966693,1289829,882.416083 +SecY,95,102,LTVVHPTL,,,6,879.5298,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,2,4.9558,16001137,441.749853 +SecY,96,102,TVVHPTL,,,5,766.4458,SecA,0.25,011118_SecA_15sec_2,1,4.937173,15866,766.801624 +SecY,96,102,TVVHPTL,,,5,766.4458,SecA,0.25,011118_SecA_15sec_3,1,4.940951,11572,766.70426 +SecY,96,102,TVVHPTL,,,5,766.4458,SecA,1,011118_SecA_1min_1,1,4.956374,13776,766.801869 +SecY,96,102,TVVHPTL,,,5,766.4458,SecA,1,011118_SecA_1min_2,1,4.946895,9803,766.803519 +SecY,96,102,TVVHPTL,,,5,766.4458,SecA,1,011118_SecA_1min_3,1,4.538343,14022,772.413651 +SecY,96,102,TVVHPTL,,,5,766.4458,SecA,5,011118_SecA_5min_1,2,4.418708,13580,384.105802 +SecY,96,102,TVVHPTL,,,5,766.4458,SecA,5,011118_SecA_5min_2,2,4.429112,12379,384.032679 +SecY,96,102,TVVHPTL,,,5,766.4458,SecA,5,011118_SecA_5min_3,2,4.436841,11848,384.017767 +SecY,96,102,TVVHPTL,,,5,766.4458,SecA,30.000002,011118_SecA_30min_1,2,4.456065,13454,384.112603 +SecY,96,102,TVVHPTL,,,5,766.4458,SecA,30.000002,011118_SecA_30min_2,1,4.703526,193858,766.924851 +SecY,96,102,TVVHPTL,,,5,766.4458,SecA,30.000002,011118_SecA_30min_3,1,4.525292,12367,774.450522 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,0,291018_SecA_SecYEG_REF_3,1,4.431045,70621,766.76118 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,0,291018_SecA_SecYEG_REF_2,1,4.434328,51396,766.799275 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,0,291018_SecA_YEG_REF_1,1,4.408588,54394,766.763266 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,0,011118_SecA_YEG_ref_2,1,4.40112,197718,766.830302 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,0,011118_SecA_YEG_ref_3,1,4.401514,172957,766.780981 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,0,011118_SecA_YEG_ref_1,1,4.414828,144540,766.792975 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,0.25,011118_SecA_YEG_15sec_1,1,4.422157,41984,767.737207 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,0.25,011118_SecA_YEG_15sec_2,1,4.408335,80383,767.623089 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,0.25,011118_SecA_YEG_15sec_3,1,4.408396,125614,767.6749 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,1,011118_SecA_YEG_1min_1,1,4.412384,112384,767.892684 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,1,011118_SecA_YEG_1min_2,1,4.402687,88938,767.808677 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,1,011118_SecA_YEG_1min_3,1,4.412954,101306,767.897868 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,5,011118_SecA_YEG_5min_1,1,4.412658,142082,768.347649 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,5,011118_SecA_YEG_5min_2,1,4.408432,159539,768.342356 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,5,011118_SecA_YEG_5min_3,1,4.404844,153257,768.407722 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,30.000002,011118_SecA_YEG_30min_1,1,4.403522,159213,768.954918 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,30.000002,011118_SecA_YEG_30min_2,1,4.399629,166558,768.913534 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG,30.000002,011118_SecA_YEG_30min_3,1,4.389706,172368,768.945708 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_3,1,4.431045,70621,766.76118 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,1,4.434328,51396,766.799275 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG_ADP,0,291018_SecA_YEG_REF_1,1,4.408588,54394,766.763266 +SecY,96,102,TVVHPTL,,,5,766.4458,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,1,4.40112,197718,766.830302 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+SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,1,5.832802,507898,763.84748 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,1,5.830952,571532,763.872436 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,1,5.842183,227589,763.79685 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,1,5.850572,141261,763.740292 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,0,291018_SecA_YEG_REF_1,1,5.834831,133225,763.699425 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,1,5.828068,544460,763.791876 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,1,5.821469,570052,763.808792 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,1,5.83654,544800,763.819492 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,1,5.846819,310817,763.821649 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,1,5.84512,272712,763.766134 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,1,5.844054,229121,763.800942 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,1,5.849057,410639,763.807758 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,1,5.848732,406724,763.83277 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,1,5.851549,363970,763.783203 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,1,5.844303,621960,763.866301 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,1,5.842647,663574,763.807508 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,1,5.845509,518305,763.837052 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,1,5.838777,716908,763.814893 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,1,5.84395,690734,763.817311 +SecY,172,177,MWLGEQ,,,5,763.3443,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,1,5.839968,641496,763.784811 +SecY,172,177,MWLGEQ,,,5,763.3443,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_2,2,5.535336,10016,382.881495 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecA,1,011118_SecA_1min_1,3,4.311615,8915,376.377992 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,0,291018_SecA_SecYEG_REF_3,2,4.137312,1891722,559.050019 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,0,291018_SecA_SecYEG_REF_2,2,4.131375,1048500,559.093358 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,0,291018_SecA_YEG_REF_1,2,4.088478,1083508,559.039948 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,0,011118_SecA_YEG_ref_2,2,4.099612,6738390,559.059018 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,0,011118_SecA_YEG_ref_3,2,4.092019,5469518,559.032171 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,0,011118_SecA_YEG_ref_1,2,4.10254,5566792,559.072849 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,0.25,011118_SecA_YEG_15sec_1,2,4.114208,1449038,559.382172 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,0.25,011118_SecA_YEG_15sec_2,2,4.09681,4185236,559.356123 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,0.25,011118_SecA_YEG_15sec_3,2,4.101755,4715328,559.335663 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,1,011118_SecA_YEG_1min_1,2,4.110227,4332754,559.52539 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,1,011118_SecA_YEG_1min_2,2,4.093639,3857839,559.642929 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,1,011118_SecA_YEG_1min_3,2,4.092559,3552871,559.615133 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,5,011118_SecA_YEG_5min_1,2,4.110338,7033036,560.104122 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,5,011118_SecA_YEG_5min_2,2,4.11171,6502681,560.124812 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,5,011118_SecA_YEG_5min_3,2,4.099807,3625121,560.108434 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,30.000002,011118_SecA_YEG_30min_1,2,4.101087,3469499,560.946093 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,30.000002,011118_SecA_YEG_30min_2,2,4.085717,4604809,560.946343 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG,30.000002,011118_SecA_YEG_30min_3,2,4.078965,4811892,560.949862 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_3,2,4.137312,1891722,559.050019 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,2,4.131375,1048500,559.093358 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,0,291018_SecA_YEG_REF_1,2,4.088478,1083508,559.039948 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,2,4.099612,6738390,559.059018 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,2,4.092019,5469518,559.032171 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,2,4.10254,5566792,559.072849 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,2,4.119242,1554361,559.652505 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,2,4.107084,1525659,559.643262 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,2,4.111772,1287852,559.578427 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,2,4.11497,1765875,559.864395 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,2,4.100792,2208053,559.935367 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,2,4.121896,1722128,559.962478 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,2,4.108946,3625452,560.512287 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,2,4.110329,2479614,560.490512 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,2,4.095983,3412791,560.575074 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,2,4.090285,2681721,561.355335 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,2,4.104516,2646554,561.373543 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,2,4.092491,2885168,561.345008 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,2,4.137312,1891722,559.050019 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,2,4.131375,1048500,559.093358 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,0,291018_SecA_YEG_REF_1,2,4.088478,1083508,559.039948 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,2,4.099612,6738390,559.059018 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,2,4.092019,5469518,559.032171 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,2,4.10254,5566792,559.072849 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,2,4.137646,1871509,559.710697 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,2,4.120681,1476857,559.83063 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,2,4.140331,1186636,559.787033 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,2,4.12185,3080900,560.073778 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,2,4.132622,1624266,560.108628 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,2,4.1435,2110809,560.132379 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,2,4.102052,4632635,560.508157 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,2,4.124774,3556969,560.576709 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,2,4.127979,3047003,560.415672 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,2,4.112268,4218968,561.432244 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,2,4.10042,4659279,561.35784 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,2,4.112254,3218047,561.249238 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecA_ADP,0.25,311018_SecA_ADP_15sec_3,3,4.297656,11981,376.112542 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_3,3,4.371539,29591,376.206737 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_3,3,4.356445,30876,376.296579 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_2,3,4.344319,38134,376.386152 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_2,3,4.316956,14295,376.438318 +SecY,178,188,ITERGIGNGIS,,,10,1116.6008,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_1,3,4.336025,17323,376.710793 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG,0,291018_SecA_SecYEG_REF_3,1,7.026602,882062,505.763612 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG,0,291018_SecA_SecYEG_REF_2,1,7.024096,810537,505.688254 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG,0,291018_SecA_YEG_REF_1,1,7.002026,829200,505.662725 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG,0,011118_SecA_YEG_ref_2,1,6.994261,6890805,505.536567 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+SecY,189,192,IIIF,,,3,505.3384,SecAYEG,5,011118_SecA_YEG_5min_3,1,7.002122,5932058,505.582879 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG,30.000002,011118_SecA_YEG_30min_1,1,6.989996,5558068,505.541358 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG,30.000002,011118_SecA_YEG_30min_2,1,7.003384,7403728,505.572187 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG,30.000002,011118_SecA_YEG_30min_3,1,6.985252,6811300,505.611941 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_3,1,7.026602,882062,505.763612 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,1,7.024096,810537,505.688254 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG_ADP,0,291018_SecA_YEG_REF_1,1,7.002026,829200,505.662725 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,1,6.994261,6890805,505.536567 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,1,6.997881,6846970,505.537235 +SecY,189,192,IIIF,,,3,505.3384,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,1,7.023149,5543959,505.540981 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+SecY,336,345,VFNPRETADN,,,8,1162.5487,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,2,3.718586,599596,583.291491 +SecY,336,345,VFNPRETADN,,,8,1162.5487,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,2,3.76277,37782,583.409996 +SecY,336,345,VFNPRETADN,,,8,1162.5487,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,2,3.708659,972705,583.435927 +SecY,336,345,VFNPRETADN,,,8,1162.5487,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,2,3.728147,831659,583.376472 +SecY,336,345,VFNPRETADN,,,8,1162.5487,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,2,3.63574,82839,583.400523 +SecY,336,345,VFNPRETADN,,,8,1162.5487,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,2,3.771836,40786,583.464618 +SecY,336,345,VFNPRETADN,,,8,1162.5487,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,2,3.764004,50239,583.296279 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,0,291018_SecA_SecYEG_REF_3,2,4.876647,3089972,638.649883 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,0,291018_SecA_SecYEG_REF_2,2,4.903184,2185464,638.648662 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,0,291018_SecA_YEG_REF_1,2,4.866231,1935413,638.600642 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,0,011118_SecA_YEG_ref_2,2,4.86055,11245146,638.638259 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,0,011118_SecA_YEG_ref_3,2,4.86158,9825177,638.642053 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,0,011118_SecA_YEG_ref_1,2,4.869859,8263591,638.656512 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,0.25,011118_SecA_YEG_15sec_1,2,4.876122,2371126,639.903646 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,0.25,011118_SecA_YEG_15sec_2,2,4.862399,5668159,639.847876 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,0.25,011118_SecA_YEG_15sec_3,2,4.862291,6690778,639.839438 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,1,011118_SecA_YEG_1min_1,2,4.868669,7044617,639.97972 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,1,011118_SecA_YEG_1min_2,2,4.84935,6427360,640.009309 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,1,011118_SecA_YEG_1min_3,2,4.8705,5008397,640.043027 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,5,011118_SecA_YEG_5min_1,2,4.852211,9637219,640.216064 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,5,011118_SecA_YEG_5min_2,2,4.856332,7766253,640.222625 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,5,011118_SecA_YEG_5min_3,2,4.855535,8527368,640.202678 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,30.000002,011118_SecA_YEG_30min_1,2,4.852929,9764313,640.653317 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,30.000002,011118_SecA_YEG_30min_2,2,4.845056,11114622,640.62506 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG,30.000002,011118_SecA_YEG_30min_3,2,4.837207,11154367,640.586949 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_3,2,4.876647,3089972,638.649883 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,2,4.903184,2185464,638.648662 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,0,291018_SecA_YEG_REF_1,2,4.866231,1935413,638.600642 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,2,4.86055,11245146,638.638259 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,2,4.86158,9825177,638.642053 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,2,4.869859,8263591,638.656512 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,2,4.870768,3848232,639.983598 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,2,4.866103,3009009,639.954061 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,2,4.870709,2096324,640.076655 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,2,4.875484,3633522,640.006627 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,2,4.876991,4200785,640.066941 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,2,4.877254,3121436,640.114298 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,2,4.866202,4414013,640.338946 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,2,4.86839,8120083,640.398912 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,2,4.85582,4346321,640.549376 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,2,4.866169,3721249,640.475512 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,2,4.85452,6539726,640.594911 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,2,4.876647,3089972,638.649883 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,2,4.903184,2185464,638.648662 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,0,291018_SecA_YEG_REF_1,2,4.866231,1935413,638.600642 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,2,4.86055,11245146,638.638259 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,2,4.86158,9825177,638.642053 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,2,4.869859,8263591,638.656512 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,2,4.890213,3567407,639.796969 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,2,4.889489,3749680,639.7842 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,2,4.899277,2888801,639.931644 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,2,4.896622,6619648,639.936527 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,2,4.887222,4196644,640.011906 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,2,4.90949,4854968,639.97231 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_3,2,4.875676,8804059,640.154712 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,2,4.891647,7690216,640.074864 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,2,4.889996,5984974,640.08179 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,2,4.878335,9397722,640.228133 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,2,4.885368,7842827,640.171102 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,2,4.88025,8453926,640.20977 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecA_ADP,5,311018_SecA_ADP_5min_2,1,5.17251,3453,1288.272338 +SecY,336,346,VFNPRETADNL,,,9,1275.6328,SecA_ADP,30.000002,311018_SecA_ADP_30min_1,1,5.180513,4445,1289.120796 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,0,291018_SecA_SecYEG_REF_3,1,3.856376,122615,533.449637 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,0,291018_SecA_SecYEG_REF_2,1,3.866234,90056,533.462174 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,0,291018_SecA_YEG_REF_1,1,3.815499,65592,533.448101 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,0,011118_SecA_YEG_ref_2,1,3.80845,317035,533.459813 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,0,011118_SecA_YEG_ref_3,1,3.812747,290491,533.490425 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,0,011118_SecA_YEG_ref_1,1,3.821601,291857,533.481838 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,0.25,011118_SecA_YEG_15sec_1,1,3.837464,120536,533.633373 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,0.25,011118_SecA_YEG_15sec_2,1,3.828633,206771,533.744086 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,0.25,011118_SecA_YEG_15sec_3,1,3.826661,276779,533.718413 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,1,011118_SecA_YEG_1min_1,1,3.847295,291479,533.859834 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,1,011118_SecA_YEG_1min_2,1,3.813854,256410,533.826011 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,1,011118_SecA_YEG_1min_3,1,3.812285,243195,533.811319 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,5,011118_SecA_YEG_5min_1,1,3.818834,342603,534.063867 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,5,011118_SecA_YEG_5min_2,1,3.846589,352265,534.105223 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,5,011118_SecA_YEG_5min_3,1,3.833641,344416,534.111171 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,30.000002,011118_SecA_YEG_30min_1,1,3.839723,306420,534.49888 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,30.000002,011118_SecA_YEG_30min_2,1,3.813042,415407,534.424583 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG,30.000002,011118_SecA_YEG_30min_3,1,3.798688,397001,534.504634 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_3,1,3.856376,122615,533.449637 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,0,291018_SecA_SecYEG_REF_2,1,3.866234,90056,533.462174 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,0,291018_SecA_YEG_REF_1,1,3.815499,65592,533.448101 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,0,011118_SecA_YEG_ref_2,1,3.80845,317035,533.459813 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,0,011118_SecA_YEG_ref_3,1,3.812747,290491,533.490425 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,0,011118_SecA_YEG_ref_1,1,3.821601,291857,533.481838 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_3,1,3.849919,173371,533.775777 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_2,1,3.814927,144274,533.711677 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,0.25,311018_SecA_YEG_ADP_15sec_1,1,3.839769,115826,533.712767 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_3,1,3.840191,180373,533.937575 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_2,1,3.80412,194052,533.910639 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,1,311018_SecA_YEG_ADP_1min_1,1,3.857762,154942,533.969111 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_3,1,3.839016,244010,534.278956 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_2,1,3.842625,281132,534.256557 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,5,311018_SecA_YEG_ADP_5min_1,1,3.825422,233828,534.231071 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_3,1,3.812525,261154,534.455337 +SecY,342,346,TADNL,,,4,533.2566,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,1,3.819261,296669,534.487529 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+SecY,429,438,YESALKKANL,,,9,1136.631,SecA,0.25,011118_SecA_15sec_2,2,4.123908,5819,569.107513 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA,0.25,011118_SecA_15sec_3,2,4.47399,343974,572.838404 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA,1,011118_SecA_1min_1,2,4.516883,283309,572.947114 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA,1,011118_SecA_1min_2,2,4.513506,319903,572.845952 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA,1,011118_SecA_1min_2,3,4.530737,42584,380.192707 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA,1,011118_SecA_1min_3,2,4.525738,40749,571.561234 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA,5,011118_SecA_5min_1,2,4.507919,544020,573.309272 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA,5,011118_SecA_5min_2,2,4.382683,16253,572.513558 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA,5,011118_SecA_5min_3,2,4.393261,15139,572.136542 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA,30.000002,011118_SecA_30min_1,2,4.672436,20361,570.485622 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+SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_2,2,4.125471,465890,571.888823 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_ADP,30.000002,311018_SecA_YEG_ADP_30min_1,2,4.122746,512243,571.855199 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_3,2,4.182008,364314,569.110651 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,0,291018_SecA_SecYEG_REF_2,2,4.184011,256875,569.185661 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,0,291018_SecA_YEG_REF_1,2,4.152394,273822,569.191057 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_2,2,4.120287,1160793,568.943957 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_3,2,4.130917,679063,569.012087 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,0,011118_SecA_YEG_ref_1,2,4.141397,703486,569.019827 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_3,2,4.205216,529864,572.004964 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_2,2,4.199866,491570,571.971491 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,0.25,291018_SecYEGA_AMPPNP_15sec_1,2,4.205101,537920,571.988235 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_3,2,4.201366,737137,571.961681 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_2,2,4.204551,564221,572.00041 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,1,291018_SecYEGA_AMPPNP_1min_1,2,4.212775,559863,572.037932 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_2,2,4.175781,619012,571.778484 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,5,301018_SecYEGA_AMPPNP_5min_1,2,4.171919,604112,571.867189 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_3,2,4.175372,839506,571.804443 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_2,2,4.16905,772511,571.812322 +SecY,429,438,YESALKKANL,,,9,1136.631,SecAYEG_AMPPNP,30.000002,301018_SecYEGA_AMPPNP_30min_1,2,4.167359,635771,571.858653 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,0.25,311018_SecA_ADP_15sec_4,2,4.522315,234546,573.082216 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,0.25,311018_SecA_ADP_15sec_3,1,4.49795,6479,1143.727612 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,0.25,311018_SecA_ADP_15sec_3,2,4.509761,400804,572.88128 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,0.25,301018_SecA_ADP_15sec_2,2,4.1869,7604,572.642752 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,1,311018_SecA_ADP_1min_3,2,4.551866,61299,572.238158 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,1,311018_SecA_ADP_1min_3,3,4.568298,62082,380.198165 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,1,311018_SecA_ADP_1min_2,2,4.540386,295873,572.920642 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,1,311018_SecA_ADP_1min_2,3,4.533258,12284,379.718671 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,1,311018_SecA_ADP_1min_1,2,4.550186,270784,573.172729 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,5,311018_SecA_ADP_5min_3,2,4.386834,24085,572.092401 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,5,311018_SecA_ADP_5min_2,2,4.391625,31417,572.29737 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,5,311018_SecA_ADP_5min_1,2,4.369931,11472,572.985238 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,30.000002,311018_SecA_ADP_30min_3,2,4.381028,18780,572.177434 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,30.000002,311018_SecA_ADP_30min_2,2,4.395677,21308,572.590628 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_ADP,30.000002,311018_SecA_ADP_30min_1,1,4.256219,10533,1137.068723 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_3,2,4.606201,38144,573.110497 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_2,2,4.389245,52655,572.274261 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_AMPPNP,0.25,291018_SecA_AMPPNP_15sec_1,2,4.525706,142952,572.873588 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_2,2,4.405489,66610,572.339982 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_AMPPNP,1,291018_SecA_AMPPNP_1min_1,2,4.40866,58017,572.686599 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_3,2,4.188464,11905,574.027836 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_AMPPNP,5,291018_SecA_AMPPNP_5min_1,2,4.40222,44843,572.155237 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_3,2,4.409287,12741,573.959896 +SecY,429,438,YESALKKANL,,,9,1136.631,SecA_AMPPNP,30.000002,291018_SecA_AMPPNP_30min_1,2,4.412867,25993,572.170605 From 572dae746c5fd0797fc71a60bbbc47acdbed4adf Mon Sep 17 00:00:00 2001 From: "Oliver M. Crook" Date: Tue, 15 Feb 2022 16:38:22 +0000 Subject: [PATCH 02/12] fix typo in forest plot --- R/functionalAnalysis-plots.R | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/R/functionalAnalysis-plots.R b/R/functionalAnalysis-plots.R index 380f25a..6ac07a8 100644 --- a/R/functionalAnalysis-plots.R +++ b/R/functionalAnalysis-plots.R @@ -693,7 +693,7 @@ forestPlot <- function(params, condition = c(1,2)) { model2 <- params@alternative@nlsmodels[[2]] tmp <- summary(model1)$parameters - tmp2 <- summary(model1)$parameters + tmp2 <- summary(model2)$parameters df <- data.frame(tmp) df$rownames <- rownames(df) From 5e83a1f52a9fe095b94304ae3f11d4e1668f5cec Mon Sep 17 00:00:00 2001 From: "Oliver M. Crook" Date: Wed, 16 Feb 2022 12:35:56 +0000 Subject: [PATCH 03/12] new case study --- vignettes/hdxstats-secA-casestudy.Rmd | 307 +++ vignettes/hdxstats-secA-casestudy.html | 2645 ++++++++++++++++++++++++ 2 files changed, 2952 insertions(+) create mode 100644 vignettes/hdxstats-secA-casestudy.Rmd create mode 100644 vignettes/hdxstats-secA-casestudy.html diff --git a/vignettes/hdxstats-secA-casestudy.Rmd b/vignettes/hdxstats-secA-casestudy.Rmd new file mode 100644 index 0000000..9c479dd --- /dev/null +++ b/vignettes/hdxstats-secA-casestudy.Rmd @@ -0,0 +1,307 @@ +--- +title: "SecA hydrogen deuterium exchange mass spectrometry data" +author: +- name: Oliver M. Crook +package: hdxstats +output: + BiocStyle::html_document: + toc_float: yes +abstract: "This vignette describes how to analyse a mass-spectrometry based hydrogen + deuterium exchange experiment, in particular we focus on empirical Bayes functional + models and visualisations. This vignette descibes a case-study for analysing + secA protein \n" +vignette: | + %\VignetteIndexEntry{SecA hydrogen deuterium exchange mass spectrometry data} + %\VignetteEngine{knitr::rmarkdown} + %%\VignetteKeywords{Mass Spectrometry, MS, MSMS, Proteomics, Metabolomics, Infrastructure, Quantitative} %\VignetteEncoding{UTF-8} +--- + +```{r style, echo = FALSE, results = 'asis'} +BiocStyle::markdown() +knitr::opts_chunk$set(dpi=300) +``` + +# Introduction + +Ahdash et al. performed HDX-MS on the baceterial sec translocon, a multi-protein +complex responsible for translocating diverse proteins across the plasma +membrane. Here we analyse this experiment using our functional data analysis +approaches + +# loading packges + +We will first load the packages required in the analysis of this approach. +Please install these packages if you do not have them. + +```{r env, message = FALSE, warning = FALSE, echo = FALSE} +library("hdxstats") +library("dplyr") +library("ggplot2") +library("RColorBrewer") +library("tidyr") +library("pheatmap") +library("scales") +library("viridis") +library("patchwork") +library("Biostrings") +library("knitr") +``` + +# Data + +The data is stored as a .csv, we need to covert it to an object of class +`QFeatures`. This is performed in a number of steps which we detail below. The +data is contained within the package and so can be loaded by specificy +the correct path. + +```{r,} +secApath <- system.file("extdata", "Project_2_SecA_Cluster_Data.csv", + package = "hdxstats") +``` + +We can now read in the .csv file and have a quick look at the .csv. +```{r,} +secA <- read.csv(secApath) +head(secA) # have a look +length(unique(secA$Sequence)) # peptide sequences +unique(secA$State) # States +``` +Let us have a quick visualisation of some the data so that we can see some of +the features + +```{r,} +filter(secA, Sequence == unique(secA$Sequence[1]), z == 1) %>% + ggplot(aes(x = Exposure, y = Center, color = factor(State))) + + theme_classic() + geom_point(size = 2) + + scale_color_manual(values = brewer.pal(n = 7, name = "Set2")) + + labs(color = "experiment", x = "Deuterium Exposure", y = "Peptide Mass") +``` +# Parsing to an object of class QFeatures + +Working from a .csv is likely to cause issues downstream. Indeed, we run +the risk of accidently changing the data or corrupting the file in some way. +Secondly, all .csvs will be formatted slightly different and so making extensible +tools for these files will be inefficient. Furthermore, working with a generic +class used in other mass-spectrometry fields can speed up analysis and adoption +of new methods. We will work the class `QFeatures` from the `QFeatures` class +as it is a powerful and scalable way to store quantitative mass-spectrometry data. + +Firstly, the data is storted in long format rather than wide format. We first +switch the data to wide format. +```{r,} +secA <- pivot_wider(data.frame(secA), + values_from = "Center", + names_from = c("Exposure", "File", "State"), + id_cols = c("Sequence", "z")) +head(secA) + +``` + +We notice that there are many columns with `NA`s. The follow code chunk removes +these columns. +```{r,} +secA <- secA[, colSums(is.na(secA)) != nrow(secA)] +``` + +We also note that the colnames are not very informative. We are going to format +in a very specific way so that later functions can automatically infer the design +from the column names. We provide in the format X(time)rep(replicate)cond(condition) +```{r,} +colnames(secA[-c(1,2)]) + +new.colnames <- gsub("0_", "0rep", paste0("X", colnames(secA)[-c(1,2)])) +new.colnames <- gsub("X0.25_", "X15rep", new.colnames) # convert to seconds too +new.colnames <- gsub("X1_", "X60rep", new.colnames) # convert to seconds too +new.colnames <- gsub("X5_", "X300rep", new.colnames) # convert to seconds too +new.colnames <- gsub("X30.000002_", "X1800rep", new.colnames) # convert to seconds too +new.colnames <- gsub(new.colnames, pattern = "rep.*?SecA_", replacement = "rep") +new.colnames <- gsub(new.colnames, pattern = "rep.*?_[0-9]_", replacement = "repcond") +repnumber <- c(seq.int(5), rep(c(1,2,3), times = 19), + seq.int(5), rep(c(1,2,3), times = 4), + seq.int(5), rep(c(1,2,3), times = 4), rep(c(4,5,6), each = 3)) +new.colnames <- stringr::str_replace_all(string = new.colnames, + pattern = "rep", + replacement = paste0("rep", repnumber)) +new.colnames +``` +We will now parse the data into an object of class `QFeatures`, we have provided +a function to assist with this in the package. If you want to do this yourself +use the `readQFeatures` function from the `QFeatures` package. +```{r,} +secAqDF <- parseDeutData(object = DataFrame(secA), + design = new.colnames, + quantcol = 3:105, sequence = "Sequence", charge = "z") +``` + +# normalisation + +We normalise the data to uptake values based on the "undeuterated" mass of the +peptide + +```{r,} +secA_newdf <- DataFrame(data.frame(assay(secAqDF) - apply(assay(secAqDF), 1, function(x) min(x, na.rm = TRUE)))) +secA_newdf <- cbind(DataFrame(secA)[,1:2], secA_newdf) + +secAqDF_norm <- parseDeutData(secA_newdf, design = new.colnames, quantcol = 3:105, sequence = "Sequence", charge = "z") +``` + + +Normalised by exchange amides + +```{r,} +secAqDF_norm1 <- normalisehdx(secAqDF_norm, + sequence = unique(secA$Sequence), + method = "pc") + +``` + +# Heatmap visualisations of HDX data + +To help us get used to the `QFeatures` we show how to generate a heatmap +of these data from this object: + +```{r, fig.height = 16, fig.width = 20, fig.align = "center"} +pheatmap(t(assay(secAqDF_norm1)), + cluster_rows = FALSE, + cluster_cols = FALSE, + color = brewer.pal(n = 9, name = "BuPu"), + main = "secA heatmap", + fontsize = 14, + legend_breaks = c(0, 1, 2, 3, 4, 5, 6, max(assay(secAqDF))), + legend_labels = c("0", "1", "2", "3", "4", "5", "6", "Incorporation")) +``` +# Analysis + +To simplify the analysis, we subset to examine the protein secA and seA bound +to ADP. + +```{r,} +secAqDF_norm1_sub <- secAqDF_norm1[,c(1:17, 63:79)] +``` + +# Functional data analysis of HDX-MS data + +The `hdxstats` package uses an empirical Bayes functional approach to analyse +the data. We explain this idea in steps so that we can get an idea of the approach. +First we fit the parametric model to the data. This will allow us to explore +the `HdxStatModel` class. + +```{r,} +res <- differentialUptakeKinetics(object = secAqDF_norm1_sub, #provide a QFeature object + feature = rownames(secAqDF_norm1_sub)[[1]][5], # which peptide to do we fit + start = list(a = NULL, b = NULL, d = NULL, p = 1)) # what are the starting parameter guesses +``` +Here, we see the `HdxStatModel` class, and that a Functional Model was applied +to the data and a total of 2 models were fitted. +```{r,} +res +``` +The `nullmodel` and `alternative` slots of an instance of `HdxStatModel` provide +the underlying fitted models. The `method` and `formula` slots provide vital +information about what analysis was performed. The `vis` slot provides a `ggplot` +object so that we can visualise the functional fits. +```{r,} +res@vis +``` + +Since this is a ggplot object, we can customise in the usual grammatical ways. +```{r,} +res@vis + scale_color_manual(values = brewer.pal(n = 8, name = "Set2")[-c(1,2)]) +``` + +# Analysis of secA HDX-MS experiment + +We have seen the basic aspects of our functional modelling approach. We now +wish to roll out our method across all peptides in the experiment. The +`fitUptakeKinetics` function allows us to apply our modelling approach across +all the peptide in the experiment. We need to provide a `QFeatures` object +and the features for which we are fitting the model. The design will be extracted +from the column names or you can provide a design yourself. The parameter +initilisation should also be provided. Sometimes the model can't be fit on the +kinetics. This is either because there is not enough data or through lack of +convergence. An error will be reported in these cases but this should not +perturb the user. You may wish to try a few starting values if there +excessive models that fail fitting. + +```{r,} +res <- fitUptakeKinetics(object = secAqDF_norm1_sub, + feature = rownames(secAqDF_norm1_sub)[[1]], + start = list(a = NULL, b = NULL, d = NULL, p = 1)) + +``` + +The code chunk above returns a class `HdxStatModels` indicating that a number +of models for peptide have been fit. This is simply a holder for a list +of `HdxStatModel` instances. +```{r,} +res +``` + +We can easily examine individual fits by going to the underyling `HdxStatModel` +class: +```{r,} +res@statmodels[[4]]@vis +``` + + +We now wish to apply statistical analysis to these fitted curves. Our approach +is an empirical Bayes testing procedure, which borrows information across peptides +to stablise variance estimates. Here, we need to provide the original data +that was analysed and the `HdxStatModels` class. The following code chunk +returns an object of class `HdxStatRes`. This object tell us that statistical +analysis was performed using our Functional model. + +```{r,} +out <- processFunctional(object = secAqDF_norm1_sub, params = res) +out +``` + +The main slot of interest is the `results` slot which returns quantities of +interest such as `p-values` and `fdr` corrected p-values because of multiple testing. +The following is the `DataFrame` of interest. +```{r,} +out@results +``` + +We can now examine the peptides for which the false discovery rate is less +than 0.01 + +```{r,} +which(out@results$ebayes.fdr < 0.01) +``` +Let us visualise some of these examples: +```{r,} +res@statmodels[[24]]@vis + res@statmodels[[25]]@vis + +``` + +We an use a forest plot to examine the differneces + +```{r,} +fp <- forestPlot(params = res@statmodels[[25]], condition = c(1, 2)) +``` + + +We can produce a table to actual numbers. We see that at all 4 timepoints +the deuterium difference is negative, though the confidence intervals overlap +with 0. Our functional approach is picking up this small but reproducible difference. +```{r,} +knitr::kable(fp$data) +``` + +We can make a Manhattan plot to better spatially visualize what's happening. + +```{r, fig.width= 22, fig.height = 15} +#We need to provide an indication of "difference" so we can examine deprotected +# or prected regions +diffdata <- assay(secAqDF_norm1_sub)[,34] - assay(secAqDF_norm1_sub)[,17] +secA <- read.csv(secApath) + +sigplots <- manhattanplot(params = out, + sequences = unique(paste0(secA$Sequence, "_", secA$z)), + region = secA[, c("Start", "End")], + difference = diffdata, + nrow = 1) +sigplots[[1]] + plot_layout(guides = 'collect') +``` + diff --git a/vignettes/hdxstats-secA-casestudy.html b/vignettes/hdxstats-secA-casestudy.html new file mode 100644 index 0000000..3f67900 --- /dev/null +++ b/vignettes/hdxstats-secA-casestudy.html @@ -0,0 +1,2645 @@ + + + + + + + + + + + + + + +SecA hydrogen deuterium exchange mass spectrometry data + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+ + + +
+
+
+
+
+ +
+ + + + + + + + + +
+

1 Introduction

+

Ahdash et al. performed HDX-MS on the baceterial sec translocon, a multi-protein +complex responsible for translocating diverse proteins across the plasma +membrane. Here we analyse this experiment using our functional data analysis +approaches

+
+
+

2 loading packges

+

We will first load the packages required in the analysis of this approach. +Please install these packages if you do not have them.

+
+
+

3 Data

+

The data is stored as a .csv, we need to covert it to an object of class +QFeatures. This is performed in a number of steps which we detail below. The +data is contained within the package and so can be loaded by specificy +the correct path.

+
secApath <- system.file("extdata", "Project_2_SecA_Cluster_Data.csv",
+                        package = "hdxstats")
+

We can now read in the .csv file and have a quick look at the .csv.

+
secA <- read.csv(secApath)
+head(secA) # have a look
+
##   Protein Start End Sequence Modification Fragment MaxUptake      MHP State
+## 1  RANDOM     4   8    LGGTQ           NA       NA         4 475.2511  SecA
+## 2  RANDOM     4   8    LGGTQ           NA       NA         4 475.2511  SecA
+## 3  RANDOM     4   8    LGGTQ           NA       NA         4 475.2511  SecA
+## 4  RANDOM     4   8    LGGTQ           NA       NA         4 475.2511  SecA
+## 5  RANDOM     4   8    LGGTQ           NA       NA         4 475.2511  SecA
+## 6  RANDOM     4   8    LGGTQ           NA       NA         4 475.2511  SecA
+##   Exposure                File z       RT  Inten   Center
+## 1     0.00   291018_SecA_REF_5 1 3.693435 710541 475.5310
+## 2     0.00   291018_SecA_REF_4 1 4.077553  13038 476.8321
+## 3     0.00   291018_SecA_REF_3 1 3.719365 819312 475.4421
+## 4     0.00   291018_SecA_REF_2 1 3.712732 559986 475.4829
+## 5     0.00   291018_SecA_REF_1 1 3.677982 527423 475.5147
+## 6     0.25 011118_SecA_15sec_1 1 3.724814 506213 475.9036
+
length(unique(secA$Sequence)) # peptide sequences
+
## [1] 397
+
unique(secA$State) # States
+
## [1] "SecA"           "SecAYEG"        "SecAYEG_ADP"    "SecAYEG_AMPPNP"
+## [5] "SecA_ADP"       "SecA_AMPPNP"
+

Let us have a quick visualisation of some the data so that we can see some of +the features

+
filter(secA, Sequence == unique(secA$Sequence[1]), z == 1) %>%
+    ggplot(aes(x = Exposure, y = Center, color = factor(State))) + 
+    theme_classic() + geom_point(size = 2) + 
+    scale_color_manual(values = brewer.pal(n = 7, name = "Set2")) + 
+    labs(color = "experiment", x = "Deuterium Exposure", y = "Peptide Mass")
+

+# Parsing to an object of class QFeatures

+

Working from a .csv is likely to cause issues downstream. Indeed, we run +the risk of accidently changing the data or corrupting the file in some way. +Secondly, all .csvs will be formatted slightly different and so making extensible +tools for these files will be inefficient. Furthermore, working with a generic +class used in other mass-spectrometry fields can speed up analysis and adoption +of new methods. We will work the class QFeatures from the QFeatures class +as it is a powerful and scalable way to store quantitative mass-spectrometry data.

+

Firstly, the data is storted in long format rather than wide format. We first +switch the data to wide format.

+
secA <- pivot_wider(data.frame(secA),
+                        values_from = "Center",
+                        names_from = c("Exposure", "File", "State"),
+                        id_cols = c("Sequence", "z"))
+head(secA)
+
## # A tibble: 6 x 107
+##   Sequence     z `0_291018_SecA_REF_5_SecA` `0_291018_SecA_RE~ `0_291018_SecA_R~
+##   <chr>    <int>                      <dbl>              <dbl>             <dbl>
+## 1 LGGTQ        1                       476.               477.              475.
+## 2 MLIKL        1                       618.               618.              618.
+## 3 LIKLLT       1                       701.               701.              701.
+## 4 IKLLT        1                       588.               588.              588.
+## 5 IINAME       1                       691.               691.              691.
+## 6 AMEPEM       1                       708.               708.              708.
+## # ... with 102 more variables: 0_291018_SecA_REF_2_SecA <dbl>,
+## #   0_291018_SecA_REF_1_SecA <dbl>, 0.25_011118_SecA_15sec_1_SecA <dbl>,
+## #   0.25_011118_SecA_15sec_2_SecA <dbl>, 0.25_011118_SecA_15sec_3_SecA <dbl>,
+## #   1_011118_SecA_1min_1_SecA <dbl>, 1_011118_SecA_1min_2_SecA <dbl>,
+## #   1_011118_SecA_1min_3_SecA <dbl>, 5_011118_SecA_5min_1_SecA <dbl>,
+## #   5_011118_SecA_5min_2_SecA <dbl>, 5_011118_SecA_5min_3_SecA <dbl>,
+## #   30.000002_011118_SecA_30min_1_SecA <dbl>, ...
+

We notice that there are many columns with NAs. The follow code chunk removes +these columns.

+
secA <- secA[, colSums(is.na(secA)) != nrow(secA)]
+

We also note that the colnames are not very informative. We are going to format +in a very specific way so that later functions can automatically infer the design +from the column names. We provide in the format X(time)rep(replicate)cond(condition)

+
colnames(secA[-c(1,2)])
+
##   [1] "0_291018_SecA_REF_5_SecA"                              
+##   [2] "0_291018_SecA_REF_4_SecA"                              
+##   [3] "0_291018_SecA_REF_3_SecA"                              
+##   [4] "0_291018_SecA_REF_2_SecA"                              
+##   [5] "0_291018_SecA_REF_1_SecA"                              
+##   [6] "0.25_011118_SecA_15sec_1_SecA"                         
+##   [7] "0.25_011118_SecA_15sec_2_SecA"                         
+##   [8] "0.25_011118_SecA_15sec_3_SecA"                         
+##   [9] "1_011118_SecA_1min_1_SecA"                             
+##  [10] "1_011118_SecA_1min_2_SecA"                             
+##  [11] "1_011118_SecA_1min_3_SecA"                             
+##  [12] "5_011118_SecA_5min_1_SecA"                             
+##  [13] "5_011118_SecA_5min_2_SecA"                             
+##  [14] "5_011118_SecA_5min_3_SecA"                             
+##  [15] "30.000002_011118_SecA_30min_1_SecA"                    
+##  [16] "30.000002_011118_SecA_30min_2_SecA"                    
+##  [17] "30.000002_011118_SecA_30min_3_SecA"                    
+##  [18] "0_011118_SecA_YEG_ref_2_SecAYEG"                       
+##  [19] "0_011118_SecA_YEG_ref_3_SecAYEG"                       
+##  [20] "0_011118_SecA_YEG_ref_1_SecAYEG"                       
+##  [21] "0.25_011118_SecA_YEG_15sec_1_SecAYEG"                  
+##  [22] "0.25_011118_SecA_YEG_15sec_2_SecAYEG"                  
+##  [23] "0.25_011118_SecA_YEG_15sec_3_SecAYEG"                  
+##  [24] "1_011118_SecA_YEG_1min_1_SecAYEG"                      
+##  [25] "1_011118_SecA_YEG_1min_2_SecAYEG"                      
+##  [26] "1_011118_SecA_YEG_1min_3_SecAYEG"                      
+##  [27] "5_011118_SecA_YEG_5min_1_SecAYEG"                      
+##  [28] "5_011118_SecA_YEG_5min_2_SecAYEG"                      
+##  [29] "5_011118_SecA_YEG_5min_3_SecAYEG"                      
+##  [30] "30.000002_011118_SecA_YEG_30min_1_SecAYEG"             
+##  [31] "30.000002_011118_SecA_YEG_30min_2_SecAYEG"             
+##  [32] "30.000002_011118_SecA_YEG_30min_3_SecAYEG"             
+##  [33] "0_011118_SecA_YEG_ref_2_SecAYEG_ADP"                   
+##  [34] "0_011118_SecA_YEG_ref_3_SecAYEG_ADP"                   
+##  [35] "0_011118_SecA_YEG_ref_1_SecAYEG_ADP"                   
+##  [36] "0.25_311018_SecA_YEG_ADP_15sec_3_SecAYEG_ADP"          
+##  [37] "0.25_311018_SecA_YEG_ADP_15sec_2_SecAYEG_ADP"          
+##  [38] "0.25_311018_SecA_YEG_ADP_15sec_1_SecAYEG_ADP"          
+##  [39] "1_311018_SecA_YEG_ADP_1min_3_SecAYEG_ADP"              
+##  [40] "1_311018_SecA_YEG_ADP_1min_2_SecAYEG_ADP"              
+##  [41] "1_311018_SecA_YEG_ADP_1min_1_SecAYEG_ADP"              
+##  [42] "5_311018_SecA_YEG_ADP_5min_3_SecAYEG_ADP"              
+##  [43] "5_311018_SecA_YEG_ADP_5min_2_SecAYEG_ADP"              
+##  [44] "5_311018_SecA_YEG_ADP_5min_1_SecAYEG_ADP"              
+##  [45] "30.000002_311018_SecA_YEG_ADP_30min_3_SecAYEG_ADP"     
+##  [46] "30.000002_311018_SecA_YEG_ADP_30min_2_SecAYEG_ADP"     
+##  [47] "30.000002_311018_SecA_YEG_ADP_30min_1_SecAYEG_ADP"     
+##  [48] "0_011118_SecA_YEG_ref_2_SecAYEG_AMPPNP"                
+##  [49] "0_011118_SecA_YEG_ref_3_SecAYEG_AMPPNP"                
+##  [50] "0_011118_SecA_YEG_ref_1_SecAYEG_AMPPNP"                
+##  [51] "0.25_291018_SecYEGA_AMPPNP_15sec_3_SecAYEG_AMPPNP"     
+##  [52] "0.25_291018_SecYEGA_AMPPNP_15sec_2_SecAYEG_AMPPNP"     
+##  [53] "0.25_291018_SecYEGA_AMPPNP_15sec_1_SecAYEG_AMPPNP"     
+##  [54] "1_291018_SecYEGA_AMPPNP_1min_3_SecAYEG_AMPPNP"         
+##  [55] "1_291018_SecYEGA_AMPPNP_1min_2_SecAYEG_AMPPNP"         
+##  [56] "1_291018_SecYEGA_AMPPNP_1min_1_SecAYEG_AMPPNP"         
+##  [57] "5_301018_SecYEGA_AMPPNP_5min_3_SecAYEG_AMPPNP"         
+##  [58] "5_301018_SecYEGA_AMPPNP_5min_2_SecAYEG_AMPPNP"         
+##  [59] "5_301018_SecYEGA_AMPPNP_5min_1_SecAYEG_AMPPNP"         
+##  [60] "30.000002_301018_SecYEGA_AMPPNP_30min_3_SecAYEG_AMPPNP"
+##  [61] "30.000002_301018_SecYEGA_AMPPNP_30min_2_SecAYEG_AMPPNP"
+##  [62] "30.000002_301018_SecYEGA_AMPPNP_30min_1_SecAYEG_AMPPNP"
+##  [63] "0_291018_SecA_REF_5_SecA_ADP"                          
+##  [64] "0_291018_SecA_REF_4_SecA_ADP"                          
+##  [65] "0_291018_SecA_REF_3_SecA_ADP"                          
+##  [66] "0_291018_SecA_REF_2_SecA_ADP"                          
+##  [67] "0_291018_SecA_REF_1_SecA_ADP"                          
+##  [68] "0.25_311018_SecA_ADP_15sec_4_SecA_ADP"                 
+##  [69] "0.25_311018_SecA_ADP_15sec_3_SecA_ADP"                 
+##  [70] "0.25_301018_SecA_ADP_15sec_2_SecA_ADP"                 
+##  [71] "1_311018_SecA_ADP_1min_3_SecA_ADP"                     
+##  [72] "1_311018_SecA_ADP_1min_2_SecA_ADP"                     
+##  [73] "1_311018_SecA_ADP_1min_1_SecA_ADP"                     
+##  [74] "5_311018_SecA_ADP_5min_3_SecA_ADP"                     
+##  [75] "5_311018_SecA_ADP_5min_2_SecA_ADP"                     
+##  [76] "5_311018_SecA_ADP_5min_1_SecA_ADP"                     
+##  [77] "30.000002_311018_SecA_ADP_30min_3_SecA_ADP"            
+##  [78] "30.000002_311018_SecA_ADP_30min_2_SecA_ADP"            
+##  [79] "30.000002_311018_SecA_ADP_30min_1_SecA_ADP"            
+##  [80] "0_291018_SecA_REF_5_SecA_AMPPNP"                       
+##  [81] "0_291018_SecA_REF_4_SecA_AMPPNP"                       
+##  [82] "0_291018_SecA_REF_3_SecA_AMPPNP"                       
+##  [83] "0_291018_SecA_REF_2_SecA_AMPPNP"                       
+##  [84] "0_291018_SecA_REF_1_SecA_AMPPNP"                       
+##  [85] "0.25_291018_SecA_AMPPNP_15sec_3_SecA_AMPPNP"           
+##  [86] "0.25_291018_SecA_AMPPNP_15sec_2_SecA_AMPPNP"           
+##  [87] "0.25_291018_SecA_AMPPNP_15sec_1_SecA_AMPPNP"           
+##  [88] "1_291018_SecA_AMPPNP_1min_3_SecA_AMPPNP"               
+##  [89] "1_291018_SecA_AMPPNP_1min_2_SecA_AMPPNP"               
+##  [90] "1_291018_SecA_AMPPNP_1min_1_SecA_AMPPNP"               
+##  [91] "5_291018_SecA_AMPPNP_5min_3_SecA_AMPPNP"               
+##  [92] "5_291018_SecA_AMPPNP_5min_2_SecA_AMPPNP"               
+##  [93] "5_291018_SecA_AMPPNP_5min_1_SecA_AMPPNP"               
+##  [94] "30.000002_291018_SecA_AMPPNP_30min_3_SecA_AMPPNP"      
+##  [95] "30.000002_291018_SecA_AMPPNP_30min_2_SecA_AMPPNP"      
+##  [96] "30.000002_291018_SecA_AMPPNP_30min_1_SecA_AMPPNP"      
+##  [97] "0_291018_SecA_SecYEG_REF_2_SecAYEG"                    
+##  [98] "0_291018_SecA_SecYEG_REF_2_SecAYEG_ADP"                
+##  [99] "0_291018_SecA_SecYEG_REF_2_SecAYEG_AMPPNP"             
+## [100] "0_291018_SecA_YEG_REF_1_SecAYEG"                       
+## [101] "0_291018_SecA_YEG_REF_1_SecAYEG_ADP"                   
+## [102] "0_291018_SecA_YEG_REF_1_SecAYEG_AMPPNP"                
+## [103] "0_291018_SecA_SecYEG_REF_3_SecAYEG"                    
+## [104] "0_291018_SecA_SecYEG_REF_3_SecAYEG_ADP"                
+## [105] "0_291018_SecA_SecYEG_REF_3_SecAYEG_AMPPNP"
+
new.colnames <- gsub("0_", "0rep", paste0("X", colnames(secA)[-c(1,2)]))
+new.colnames <- gsub("X0.25_", "X15rep", new.colnames) # convert to seconds too
+new.colnames <- gsub("X1_", "X60rep", new.colnames) # convert to seconds too
+new.colnames <- gsub("X5_", "X300rep", new.colnames) # convert to seconds too
+new.colnames <- gsub("X30.000002_", "X1800rep", new.colnames) # convert to seconds too
+new.colnames <- gsub(new.colnames, pattern = "rep.*?SecA_", replacement = "rep")
+new.colnames <- gsub(new.colnames, pattern = "rep.*?_[0-9]_", replacement = "repcond")
+repnumber <- c(seq.int(5), rep(c(1,2,3), times = 19),
+               seq.int(5), rep(c(1,2,3), times = 4),
+               seq.int(5), rep(c(1,2,3), times = 4), rep(c(4,5,6), each = 3))
+new.colnames <- stringr::str_replace_all(string = new.colnames,
+                                pattern = "rep",
+                                replacement = paste0("rep", repnumber))
+new.colnames
+
##   [1] "X0rep1condSecA"              "X0rep2condSecA"             
+##   [3] "X0rep3condSecA"              "X0rep4condSecA"             
+##   [5] "X0rep5condSecA"              "X15rep1condSecA"            
+##   [7] "X15rep2condSecA"             "X15rep3condSecA"            
+##   [9] "X60rep1condSecA"             "X60rep2condSecA"            
+##  [11] "X60rep3condSecA"             "X300rep1condSecA"           
+##  [13] "X300rep2condSecA"            "X300rep3condSecA"           
+##  [15] "X1800rep1condSecA"           "X1800rep2condSecA"          
+##  [17] "X1800rep3condSecA"           "X0rep1condSecAYEG"          
+##  [19] "X0rep2condSecAYEG"           "X0rep3condSecAYEG"          
+##  [21] "X15rep1condSecAYEG"          "X15rep2condSecAYEG"         
+##  [23] "X15rep3condSecAYEG"          "X60rep1condSecAYEG"         
+##  [25] "X60rep2condSecAYEG"          "X60rep3condSecAYEG"         
+##  [27] "X300rep1condSecAYEG"         "X300rep2condSecAYEG"        
+##  [29] "X300rep3condSecAYEG"         "X1800rep1condSecAYEG"       
+##  [31] "X1800rep2condSecAYEG"        "X1800rep3condSecAYEG"       
+##  [33] "X0rep1condSecAYEG_ADP"       "X0rep2condSecAYEG_ADP"      
+##  [35] "X0rep3condSecAYEG_ADP"       "X15rep1condSecAYEG_ADP"     
+##  [37] "X15rep2condSecAYEG_ADP"      "X15rep3condSecAYEG_ADP"     
+##  [39] "X60rep1condSecAYEG_ADP"      "X60rep2condSecAYEG_ADP"     
+##  [41] "X60rep3condSecAYEG_ADP"      "X300rep1condSecAYEG_ADP"    
+##  [43] "X300rep2condSecAYEG_ADP"     "X300rep3condSecAYEG_ADP"    
+##  [45] "X1800rep1condSecAYEG_ADP"    "X1800rep2condSecAYEG_ADP"   
+##  [47] "X1800rep3condSecAYEG_ADP"    "X0rep1condSecAYEG_AMPPNP"   
+##  [49] "X0rep2condSecAYEG_AMPPNP"    "X0rep3condSecAYEG_AMPPNP"   
+##  [51] "X15rep1condSecAYEG_AMPPNP"   "X15rep2condSecAYEG_AMPPNP"  
+##  [53] "X15rep3condSecAYEG_AMPPNP"   "X60rep1condSecAYEG_AMPPNP"  
+##  [55] "X60rep2condSecAYEG_AMPPNP"   "X60rep3condSecAYEG_AMPPNP"  
+##  [57] "X300rep1condSecAYEG_AMPPNP"  "X300rep2condSecAYEG_AMPPNP" 
+##  [59] "X300rep3condSecAYEG_AMPPNP"  "X1800rep1condSecAYEG_AMPPNP"
+##  [61] "X1800rep2condSecAYEG_AMPPNP" "X1800rep3condSecAYEG_AMPPNP"
+##  [63] "X0rep1condSecA_ADP"          "X0rep2condSecA_ADP"         
+##  [65] "X0rep3condSecA_ADP"          "X0rep4condSecA_ADP"         
+##  [67] "X0rep5condSecA_ADP"          "X15rep1condSecA_ADP"        
+##  [69] "X15rep2condSecA_ADP"         "X15rep3condSecA_ADP"        
+##  [71] "X60rep1condSecA_ADP"         "X60rep2condSecA_ADP"        
+##  [73] "X60rep3condSecA_ADP"         "X300rep1condSecA_ADP"       
+##  [75] "X300rep2condSecA_ADP"        "X300rep3condSecA_ADP"       
+##  [77] "X1800rep1condSecA_ADP"       "X1800rep2condSecA_ADP"      
+##  [79] "X1800rep3condSecA_ADP"       "X0rep1condSecA_AMPPNP"      
+##  [81] "X0rep2condSecA_AMPPNP"       "X0rep3condSecA_AMPPNP"      
+##  [83] "X0rep4condSecA_AMPPNP"       "X0rep5condSecA_AMPPNP"      
+##  [85] "X15rep1condSecA_AMPPNP"      "X15rep2condSecA_AMPPNP"     
+##  [87] "X15rep3condSecA_AMPPNP"      "X60rep1condSecA_AMPPNP"     
+##  [89] "X60rep2condSecA_AMPPNP"      "X60rep3condSecA_AMPPNP"     
+##  [91] "X300rep1condSecA_AMPPNP"     "X300rep2condSecA_AMPPNP"    
+##  [93] "X300rep3condSecA_AMPPNP"     "X1800rep1condSecA_AMPPNP"   
+##  [95] "X1800rep2condSecA_AMPPNP"    "X1800rep3condSecA_AMPPNP"   
+##  [97] "X0rep4condSecAYEG"           "X0rep4condSecAYEG_ADP"      
+##  [99] "X0rep4condSecAYEG_AMPPNP"    "X0rep5condSecAYEG"          
+## [101] "X0rep5condSecAYEG_ADP"       "X0rep5condSecAYEG_AMPPNP"   
+## [103] "X0rep6condSecAYEG"           "X0rep6condSecAYEG_ADP"      
+## [105] "X0rep6condSecAYEG_AMPPNP"
+

We will now parse the data into an object of class QFeatures, we have provided +a function to assist with this in the package. If you want to do this yourself +use the readQFeatures function from the QFeatures package.

+
secAqDF <- parseDeutData(object = DataFrame(secA),
+                        design = new.colnames,
+                        quantcol = 3:105, sequence = "Sequence", charge = "z")
+
+
+

4 normalisation

+

We normalise the data to uptake values based on the “undeuterated” mass of the +peptide

+
secA_newdf <- DataFrame(data.frame(assay(secAqDF) - apply(assay(secAqDF), 1, function(x) min(x, na.rm = TRUE))))
+secA_newdf <- cbind(DataFrame(secA)[,1:2], secA_newdf)
+
+secAqDF_norm <- parseDeutData(secA_newdf, design = new.colnames, quantcol = 3:105, sequence = "Sequence", charge = "z")
+

Normalised by exchange amides

+
secAqDF_norm1 <- normalisehdx(secAqDF_norm,
+                             sequence = unique(secA$Sequence),
+                             method = "pc")
+
+
+

5 Heatmap visualisations of HDX data

+

To help us get used to the QFeatures we show how to generate a heatmap +of these data from this object:

+
pheatmap(t(assay(secAqDF_norm1)),
+         cluster_rows = FALSE, 
+         cluster_cols = FALSE,
+         color = brewer.pal(n = 9, name = "BuPu"),
+         main = "secA heatmap", 
+         fontsize = 14,
+         legend_breaks = c(0, 1, 2, 3, 4, 5, 6, max(assay(secAqDF))),
+         legend_labels = c("0", "1", "2", "3", "4", "5", "6", "Incorporation"))
+

+# Analysis

+

To simplify the analysis, we subset to examine the protein secA and seA bound +to ADP.

+
secAqDF_norm1_sub <- secAqDF_norm1[,c(1:17, 63:79)]
+
+
+

6 Functional data analysis of HDX-MS data

+

The hdxstats package uses an empirical Bayes functional approach to analyse +the data. We explain this idea in steps so that we can get an idea of the approach. +First we fit the parametric model to the data. This will allow us to explore +the HdxStatModel class.

+
res <- differentialUptakeKinetics(object = secAqDF_norm1_sub, #provide a QFeature object
+                                  feature = rownames(secAqDF_norm1_sub)[[1]][5], # which peptide to do we fit
+                                  start = list(a = NULL, b = NULL,  d = NULL, p = 1)) # what are the starting parameter guesses
+
## Warning in differentialUptakeKinetics(object = secAqDF_norm1_sub, feature =
+## rownames(secAqDF_norm1_sub)[[1]][5], : NAs introduced by coercion
+

Here, we see the HdxStatModel class, and that a Functional Model was applied +to the data and a total of 2 models were fitted.

+
res
+
## Object of class "HdxStatModel"
+## Method: Functional Model 
+## Fitted 2
+

The nullmodel and alternative slots of an instance of HdxStatModel provide +the underlying fitted models. The method and formula slots provide vital +information about what analysis was performed. The vis slot provides a ggplot +object so that we can visualise the functional fits.

+
res@vis
+

+

Since this is a ggplot object, we can customise in the usual grammatical ways.

+
res@vis + scale_color_manual(values = brewer.pal(n = 8, name = "Set2")[-c(1,2)])
+
## Scale for 'colour' is already present. Adding another scale for 'colour',
+## which will replace the existing scale.
+

+
+
+

7 Analysis of secA HDX-MS experiment

+

We have seen the basic aspects of our functional modelling approach. We now +wish to roll out our method across all peptides in the experiment. The +fitUptakeKinetics function allows us to apply our modelling approach across +all the peptide in the experiment. We need to provide a QFeatures object +and the features for which we are fitting the model. The design will be extracted +from the column names or you can provide a design yourself. The parameter +initilisation should also be provided. Sometimes the model can’t be fit on the +kinetics. This is either because there is not enough data or through lack of +convergence. An error will be reported in these cases but this should not +perturb the user. You may wish to try a few starting values if there +excessive models that fail fitting.

+
res <- fitUptakeKinetics(object = secAqDF_norm1_sub,
+                         feature = rownames(secAqDF_norm1_sub)[[1]],
+                         start = list(a = NULL, b = NULL,  d = NULL, p = 1))
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "too few data points to fit model"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## [1] "too few data points to fit model"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## [1] "too few data points to fit model"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## [1] "too few data points to fit model"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## [1] "too few data points to fit model"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## [1] "too few data points to fit model"
+## [1] "too few data points to fit model"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## [1] "too few data points to fit model"
+
## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
+## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
+## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
+## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## [1] "too few data points to fit model"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
+## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
+## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
+## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
+## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
+## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
+## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
+## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## [1] "too few data points to fit model"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## [1] "too few data points to fit model"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
+## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
+## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
+## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in nls.lm(par = start, fn = FCT, jac = jac, control = control, lower = lower, : lmdif: info = 0. Improper input parameters.
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+## [1] "too few data points to fit model"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## Error in nlsModel(formula, mf, start, wts) : 
+##   singular gradient matrix at initial parameter estimates
+## [1] "Could not fit model, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## [1] "too few data points to fit model"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+## [1] "too few data points to fit model"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+
## Warning in max(data$value): no non-missing arguments to max; returning -Inf
+
## Warning in min(data$value): no non-missing arguments to min; returning Inf
+
## Warning in differentialUptakeKinetics(object = object, feature = x, start =
+## start, : NAs introduced by coercion
+
## [1] "model fit failed, likely exessive missing values"
+

The code chunk above returns a class HdxStatModels indicating that a number +of models for peptide have been fit. This is simply a holder for a list +of HdxStatModel instances.

+
res
+
## Object of class "HdxStatModels"
+## Number of models 204
+

We can easily examine individual fits by going to the underyling HdxStatModel +class:

+
res@statmodels[[4]]@vis
+

+

We now wish to apply statistical analysis to these fitted curves. Our approach +is an empirical Bayes testing procedure, which borrows information across peptides +to stablise variance estimates. Here, we need to provide the original data +that was analysed and the HdxStatModels class. The following code chunk +returns an object of class HdxStatRes. This object tell us that statistical +analysis was performed using our Functional model.

+
out <- processFunctional(object = secAqDF_norm1_sub, params = res)
+
## Warning in pf(q = Fstat, df1 = d1, df2 = d2, lower.tail = FALSE): NaNs produced
+
## Warning in pf(q = modFstat, df1 = d1, df2 = d2, lower.tail = FALSE): NaNs
+## produced
+
out
+
## Object of class "HdxStatRes"
+## Analysed using Functional model
+

The main slot of interest is the results slot which returns quantities of +interest such as p-values and fdr corrected p-values because of multiple testing. +The following is the DataFrame of interest.

+
out@results
+
## DataFrame with 204 rows and 8 columns
+##                   Fstat.Fstat Fstat.numerator Fstat.denomenator       pvals
+##                        <list>          <list>            <list>   <numeric>
+## LGGTQ_1               0.39418        0.004996         0.0126744 8.10901e-01
+## IINAME_1              3.62553      0.00127486       0.000351634 1.89955e-02
+## AMEPEM_1              13.6476      0.00240748       0.000176403 4.02221e-06
+## MEPEM_1               21.7177       0.0063718       0.000293391 5.65838e-08
+## EKLSDEELKGKTAE_3       6.6861     8.53707e-05       1.27684e-05 9.17601e-04
+## ...                       ...             ...               ...         ...
+## IFQSIG_1             0.114395       0.0051095         0.0446654    0.975564
+## PGMQG_1              0.527581       0.0210774          0.039951    0.717140
+## VINPGFAF_1           0.753211      0.00637704        0.00846647    0.570388
+## VTGTMFL_1            0.219675        0.216819          0.986999    0.922320
+## VYAAQSTHLPLKVNM_3           0               0        -0.0287776         NaN
+##                           fdr ebayes.pvals  ebayes.fdr fitcomplete
+##                     <numeric>    <numeric>   <numeric>   <integer>
+## LGGTQ_1           8.52917e-01  7.97194e-01 8.38499e-01           1
+## IINAME_1          2.98922e-02  1.58705e-02 2.53678e-02           2
+## AMEPEM_1          1.27579e-05  3.02336e-06 1.04024e-05           3
+## MEPEM_1           2.87163e-07  3.84268e-08 1.95016e-07           4
+## EKLSDEELKGKTAE_3  1.80848e-03  2.18096e-03 4.02486e-03           5
+## ...                       ...          ...         ...         ...
+## IFQSIG_1             1.000000     0.971886    1.000000         200
+## PGMQG_1              0.762196     0.688158    0.731394         201
+## VINPGFAF_1           0.612639     0.534855    0.574474         202
+## VTGTMFL_1            0.955260     0.908255    0.945517         203
+## VYAAQSTHLPLKVNM_3         NaN          NaN         NaN         204
+

We can now examine the peptides for which the false discovery rate is less +than 0.01

+
which(out@results$ebayes.fdr < 0.01)
+
##                AMEPEM_1                 MEPEM_1        EKLSDEELKGKTAE_3 
+##                       3                       4                       5 
+##       EKLSDEELKGKTAEF_2            RARLEKGEVL_3           RARLEKGEVLE_3 
+##                       6                       8                       9 
+##         RARLEKGEVLENL_2              MRHFDVQL_2               DVQLLGG_1 
+##                      10                      18                      19 
+##               LLGGMVL_1              LGGMVLNE_1                 MVLNE_1 
+##                      20                      21                      22 
+##              NERSIAEM_1         MRTGEGKTLTATL_2          RTGEGKTLTATL_2 
+##                      23                      24                      25 
+##               ATLPAYL_1                  PAYL_1          NALTGKGVHVVT_2 
+##                      26                      27                      29 
+##       NALTGKGVHVVTVND_2         AQRDAENNRPLFE_2               FLGLTVG_1 
+##                      30                      31                      34 
+##                LGLTVG_1   LTVGINLPGMPAPAKREAY_3 TVGINLPGMPAPAKREAYAAD_3 
+##                      35                      36                      37 
+##    INLPGMPAPAKREAYAAD_2               LPGMPAP_1           ITYGTNNEYGF_1 
+##                      38                      39                      41 
+##          ITYGTNNEYGFD_2                 NEYGF_1                NEYGFD_1 
+##                      42                      43                      44 
+##         DYLRDNMAFSPEE_2              NMAFSPEE_1                AFSPEE_1 
+##                      45                      48                      49 
+##                 VDEVD_1                EVDSIL_1       IDEARTPLIISGPAE_3 
+##                      51                      52                      54 
+##   IDEARTPLIISGPAEDSSE_2          ARTPLIISGPAE_2      ARTPLIISGPAEDSSE_2 
+##                      55                      56                      57 
+##               FQGEGHF_2                 GEGHF_1              LLVKEGIM_1 
+##                      60                      61                      66 
+##            LLVKEGIMDE_2               LVKEGIM_1             LVKEGIMDE_1 
+##                      67                      69                      70 
+##          LVKEGIMDEGES_2         LVKEGIMDEGESL_2               YSPANIM_1 
+##                      71                      72                      75 
+##              MHHVTAAL_1            AKEGVQIQNE_2        AKEGVQIQNENQTL_2 
+##                      78                      85                      86 
+##      AKEGVQIQNENQTLAS_2              IQNENQTL_1               SITFQNY_1 
+##                      87                      88                      90 
+##                  FQNY_1        YEKLAGMTGTADTE_2       YEKLAGMTGTADTEA_2 
+##                      93                      96                      97 
+##                 FEFSS_1     VVVPTNRPMIRKDLPDL_4   VVVPTNRPMIRKDLPDLVY_4 
+##                      98                     103                     104 
+##       VPTNRPMIRKDLPDL_3                 VYMTE_1                VYMTEA_1 
+##                     105                     107                     108 
+##      IIEDIKERTAKGQPVL_3                  QPVL_1               VGTISIE_1 
+##                     109                     110                     111 
+##                SELVSN_1                 AAIVA_1          AAIVAQAGYPAA_1 
+##                     115                     118                     119 
+##            IVAQAGYPAA_1          IVAQAGYPAAVT_1               QAGYPAA_1 
+##                     120                     121                     122 
+##               VTIATNM_1                IVLGGS_1               IVLGGSW_1 
+##                     127                     129                     130 
+##             ALENPTAEQ_1                 EAGGL_1                  YLSM_1 
+##                     133                     137                     139 
+##                 YLSME_1         IEHPWVTKAIANA_2              FDIRKQLL_2 
+##                     140                     143                     144 
+##             FDIRKQLLE_2            FDIRKQLLEY_2         YDDVANDQRRAIY_2 
+##                     145                     146                     147 
+##          DDVANDQRRAIY_2               SQRNELL_1                DVSETI_1 
+##                     148                     149                     152 
+##                IREDVF_1         KATIDAYIPPQSL_2                WDIPGL_1 
+##                     153                     154                     158 
+##              WDIPGLQE_1            WDIPGLQERL_2                  PGLQ_1 
+##                     159                     160                     161 
+##          QERLKNDFDLDL_2               ERLKNDF_2           ERLKNDFDLDL_2 
+##                     162                     163                     164 
+##                RLKNDF_2            RLKNDFDLDL_2                  DLDL_1 
+##                     165                     166                     167 
+##               LDLPIAE_1                  PIAE_1                PIAEWL_1 
+##                     168                     169                     170 
+##              WLDKEPEL_1                 HEETL_1               HEETLRE_2 
+##                     171                     175                     176 
+##            RERILAQSIE_2              RILAQSIE_1                  LQTL_1 
+##                     177                     178                     180 
+##                QTLDSL_1              WKEHLAAM_1             WKEHLAAMD_2 
+##                     181                     183                     184 
+##                 AAMDY_1                 FAAML_1                LESLKY_1 
+##                     185                     192                     194 
+##           ESLKYEVISTL_2                EVISTL_1 
+##                     195                     196
+

Let us visualise some of these examples:

+
res@statmodels[[24]]@vis + res@statmodels[[25]]@vis
+

+

We an use a forest plot to examine the differneces

+
fp <- forestPlot(params = res@statmodels[[25]], condition = c(1, 2))
+

+

We can produce a table to actual numbers. We see that at all 4 timepoints +the deuterium difference is negative, though the confidence intervals overlap +with 0. Our functional approach is picking up this small but reproducible difference.

+
knitr::kable(fp$data)
+
+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
EstimateconfLconfUrownamescondition
a0.89081280.82081930.9608062a1
d0.0227637-0.00114660.0466741d1
p0.58467150.49300510.6763380p1
b0.03894110.02275610.0551262b1
a10.89081280.82081930.9608062a2
d10.0227637-0.00114660.0466741d2
p10.58467150.49300510.6763380p2
b10.03894110.02275610.0551262b2
10.0000000-0.01622510.0162251Timepoint 0Deuterium Difference
20.10035960.07790540.1228137Timepoint 15Deuterium Difference
30.17617360.12700210.2253451Timepoint 60Deuterium Difference
40.43589390.38364910.4881387Timepoint 300Deuterium Difference
50.57593910.47041570.6814625Timepoint 1800Deuterium Difference
+
+ + + +
+
+ +
+ + + + + + + + + + + + + + + + + From 33534a5b5a8bacbd80aae4ff336364f6ecb6b256 Mon Sep 17 00:00:00 2001 From: "Oliver M. Crook" Date: Wed, 16 Feb 2022 12:41:02 +0000 Subject: [PATCH 04/12] smll change to case study --- vignettes/hdxstats-secA-casestudy.Rmd | 10 ++++++++-- 1 file changed, 8 insertions(+), 2 deletions(-) diff --git a/vignettes/hdxstats-secA-casestudy.Rmd b/vignettes/hdxstats-secA-casestudy.Rmd index 9c479dd..a24f833 100644 --- a/vignettes/hdxstats-secA-casestudy.Rmd +++ b/vignettes/hdxstats-secA-casestudy.Rmd @@ -297,9 +297,15 @@ We can make a Manhattan plot to better spatially visualize what's happening. diffdata <- assay(secAqDF_norm1_sub)[,34] - assay(secAqDF_norm1_sub)[,17] secA <- read.csv(secApath) +#carefully subset regions and sequences +region = secA[, c("Start", "End")] +region <- unique(region[paste0(secA$Sequence, "_", secA$z) %in% rownames(out@results),]) +sequences = rownames(out@results) + + sigplots <- manhattanplot(params = out, - sequences = unique(paste0(secA$Sequence, "_", secA$z)), - region = secA[, c("Start", "End")], + sequences = sequences, + region = region, difference = diffdata, nrow = 1) sigplots[[1]] + plot_layout(guides = 'collect') From c942862935e4462ce822b40429ac9253f8d11ba1 Mon Sep 17 00:00:00 2001 From: "Oliver M. Crook" Date: Wed, 16 Feb 2022 17:49:25 +0000 Subject: [PATCH 05/12] fix namespace --- DESCRIPTION | 19 ++++++++++--------- NAMESPACE | 6 +++++- 2 files changed, 15 insertions(+), 10 deletions(-) diff --git a/DESCRIPTION b/DESCRIPTION index e858cd7..1c2d145 100644 --- a/DESCRIPTION +++ b/DESCRIPTION @@ -1,18 +1,18 @@ Package: hdxstats Title: Statistical analysis of hydrogen deuterium exchange mass spectrometry -Version: 1.0 -Author: c(person("Crook", "Oliver M.", - email = "oliver.crook@stats.ox.ac.uk", - comment = c(ORCID = "0000-0001-5669-8506"), - role = c("aut", "cre"))) -Maintainer: Oliver Crook +Version: 0.99.0 +Authors@R: c(person(family = "Crook", given = "Oliver M.", + email = "oliver.crook@stats.ox.ac.uk", + comment = c(ORCID = "0000-0001-5669-8506"), + role = c("aut", "cre"))) Description: The hdxstats package enables the analysis and visualisation of hydrogen deuterium exchange mass spectromery experiments. It supports all types of HDX-MS experiments but provides additional support for epitope - mapping experiments. + mapping experiments. The package focuses and implements empirical Bayes + functional models for hydrogen deuterium exchange data. License: Artistic-2.0 Encoding: UTF-8 -Depends: R (>= 4.0), S4Vectors, Biobase, QFeatures +Depends: R (>= 4.1), S4Vectors, Biobase, QFeatures Imports: Rcpp (>= 1.0.4.6), ggplot2, dplyr, @@ -29,7 +29,8 @@ Imports: Rcpp (>= 1.0.4.6), broom, limma, lmerTest, - harmonicmeanp + harmonicmeanp, + stats Suggests: pheatmap, viridis, scales, diff --git a/NAMESPACE b/NAMESPACE index 8034202..9f94326 100644 --- a/NAMESPACE +++ b/NAMESPACE @@ -9,7 +9,11 @@ exportPattern("^[[:alpha:]]+") importFrom("minpack.lm", "nlsLM", "nls.lm.control") importFrom("broom", "tidy") importFrom("Biobase", "exprs") - importFrom("QFeatures", "readQFeatures") + importFrom("QFeatures", "readQFeatures", "longFormat") importFrom("S4Vectors", "DataFrame") importFrom("harmonicmeanp", "p.hmp") + importFrom("RColorBrewer", "brewer.pal") + importFrom("methods", "is") +import("Rcpp", "ggplot2", "dplyr", "plyr", "knitr", "BiocParallel", "robustbase", + "BiocStyle", "stringi") exportMethods(logLik, residuals, vcov, likRatio, coef, deviance, summary, wilk) \ No newline at end of file From 771d4cfd723e83a86cab1bacfcf2cb49c2ac6b85 Mon Sep 17 00:00:00 2001 From: "Oliver M. Crook" Date: Thu, 1 Sep 2022 14:01:05 +0100 Subject: [PATCH 06/12] small fix --- R/hdxstat-methods.R | 5 + vignettes/flexible-fits.html | 674 +++++++++++++++++++++++++++++++++++ 2 files changed, 679 insertions(+) create mode 100644 vignettes/flexible-fits.html diff --git a/R/hdxstat-methods.R b/R/hdxstat-methods.R index dd536ef..8053eb8 100644 --- a/R/hdxstat-methods.R +++ b/R/hdxstat-methods.R @@ -66,6 +66,11 @@ setMethod("wilk", "HdxStatModel", setMethod("coef", "HdxStatModel", function(object) { .out <- t(sapply(c(list(object@nullmodel), object@alternative@nlsmodels), coef)) + if(dim(.out)[1] == 1){ + nm <- colnames(.out)[1] + .out <- t(.out) + colnames(.out) <- nm + } rownames(.out) <- c("null", paste0("alt", seq.int(length(object)))) .out }) diff --git a/vignettes/flexible-fits.html b/vignettes/flexible-fits.html new file mode 100644 index 0000000..f74f6e1 --- /dev/null +++ b/vignettes/flexible-fits.html @@ -0,0 +1,674 @@ + + + + + + + + + + + + + + +Fitting different functional models + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+ + + +
+
+
+
+
+ +
+ + + + + + + + + +
+

1 A well-defined HDX-MS experiment

+

This vignette describeds how to analyse time-resolved differential HDX-MS +experiments. The key elements are at least two conditions i.e. apo + antibody, +apo + small molecule or protein closed + protien open, etc. The experiment can +be replicated, though if there are sufficient time points analysed (>=3) then +occasionally signficant results can be obtained. The data provided should be +centroid-centric data. This package does not yet support analysis straight +from raw spectra. Typically this will be provided as a .csv from tools such as +dynamiX or HDExaminer.

+
+
+

2 Main elements of the package

+

The package relies of Bioconductor infrastructure so that it integrates with +other data types and can benefit from advantages in other fields of mass-spectrometry. +There are package specific object, classes and methods but importantly there is +reuse of classes found in quantitative proteomics data, mainly the QFeatures +object which extends the SummarisedExperiment class for mass spectrometry data. +The focus of this package is on testing and visualisation of the testing results.

+
+
+

3 Data

+

We will begin with a structural variant experiment in which MHP and a structural +variant were mixed in different proportions. HDX-MS was performed on these samples +and we expect to see reproducible but subtle differences. We first load the data +from the package and it is .csv format.

+
MBPpath <- system.file("extdata", "MBP.csv", package = "hdxstats")
+

We can now read in the .csv file and have a quick look at the .csv.

+
MBP <- read.csv(MBPpath)
+head(MBP) # have a look
+
##   hx_sample pep_start pep_end pep_sequence pep_charge     d confidence  score
+## 1       10%        19      30 VIWINGDKGYNG          2 2.120     medium 0.8686
+## 2       10%        19      30 VIWINGDKGYNG          2 2.146     medium 0.8173
+## 3       10%        19      30 VIWINGDKGYNG          2 2.143     medium 0.8839
+## 4       10%        19      30 VIWINGDKGYNG          2    NA       <NA>     NA
+## 5       10%        19      30 VIWINGDKGYNG          2    NA       <NA>     NA
+## 6       10%        19      30 VIWINGDKGYNG          2    NA       <NA>     NA
+##   hx_time time_unit replicate_cnt
+## 1      30         s             1
+## 2      30         s             2
+## 3      30         s             3
+## 4      30         s             4
+## 5      30         s             5
+## 6      30         s             6
+
length(unique(MBP$pep_sequence)) # peptide sequences
+
## [1] 115
+

Let us have a quick visualisation of some the data so that we can see some of +the features

+
filter(MBP, pep_sequence == unique(MBP$pep_sequence[1]), pep_charge == 2) %>%
+    ggplot(aes(x = hx_time, y = d, group = factor(replicate_cnt),
+               color = factor(hx_sample,
+                              unique(MBP$hx_sample)[c(7,5,1,2,3,4,6)]))) + 
+    theme_classic() + geom_point(size = 2) + 
+    scale_color_manual(values = brewer.pal(n = 7, name = "Set2")) + 
+    labs(color = "experiment", x = "Deuterium Exposure", y = "Deuterium incoperation")
+
## Warning: Removed 96 rows containing missing values (geom_point).
+

+We can see that the units of the time dimension are in seconds and that +Deuterium incoperation has been normalized into Daltons.

+
+
+

4 Parsing to an object of class QFeatures

+

Working from a .csv is likely to cause issues downstream. Indeed, we run +the risk of accidently changing the data or corrupting the file in some way. +Secondly, all .csvs will be formatted slightly different and so making extensible +tools for these files will be inefficient. Furthermore, working with a generic +class used in other mass-spectrometry fields can speed up analysis and adoption +of new methods. We will work the class QFeatures from the QFeatures class +as it is a powerful and scalable way to store quantitative mass-spectrometry data.

+

Firstly, the data is storted in long format rather than wide format. We first +switch the data to wide format.

+
MBP_wide <- pivot_wider(data.frame(MBP),
+                        values_from = d,
+                        names_from = c("hx_time", "replicate_cnt", "hx_sample"),
+                        id_cols = c("pep_sequence", "pep_charge"))
+head(MBP_wide)
+
## # A tibble: 6 x 198
+##   pep_sequence pep_charge `30_1_10%` `30_2_10%` `30_3_10%` `30_4_10%` `30_5_10%`
+##   <chr>             <int>      <dbl>      <dbl>      <dbl>      <dbl>      <dbl>
+## 1 VIWINGDKGYNG          2      2.12       2.15       2.14          NA         NA
+## 2 VIWINGDKGYN~          2      2.12       2.12       2.11          NA         NA
+## 3 GDKGYNGLAEVG          3      0.552      0.555      0.553         NA         NA
+## 4 LAEVGKKFEKD~          4      2.41       2.36       2.42          NA         NA
+## 5 AEVGKKFEKDT~          4      0.458      0.425      0.573         NA         NA
+## 6 TVEHPDKL              3      1.43       1.44       1.44          NA         NA
+## # ... with 191 more variables: 30_6_10% <dbl>, 30_7_10% <dbl>, 240_1_10% <dbl>,
+## #   240_2_10% <dbl>, 240_3_10% <dbl>, 240_4_10% <dbl>, 240_5_10% <dbl>,
+## #   240_6_10% <dbl>, 240_7_10% <dbl>, 1800_1_10% <dbl>, 1800_2_10% <dbl>,
+## #   1800_3_10% <dbl>, 1800_4_10% <dbl>, 1800_5_10% <dbl>, 1800_6_10% <dbl>,
+## #   1800_7_10% <dbl>, 14400_1_10% <dbl>, 14400_2_10% <dbl>, 14400_3_10% <dbl>,
+## #   14400_4_10% <dbl>, 14400_5_10% <dbl>, 14400_6_10% <dbl>, 14400_7_10% <dbl>,
+## #   30_1_15% <dbl>, 30_2_15% <dbl>, 30_3_15% <dbl>, 30_4_15% <dbl>, ...
+

We notice that there are many columns with NAs. The follow code chunk removes +these columns.

+
MBP_wide <- MBP_wide[, colSums(is.na(MBP_wide)) != nrow(MBP_wide)]
+

We also note that the colnames are not very informative. We are going to format +in a very specific way so that later functions can automatically infer the design +from the column names. We provide in the format X(time)rep(replicate)cond(condition)

+
colnames(MBP_wide)[-c(1,2)]
+
##   [1] "30_1_10%"        "30_2_10%"        "30_3_10%"        "240_1_10%"      
+##   [5] "240_2_10%"       "240_3_10%"       "1800_1_10%"      "1800_2_10%"     
+##   [9] "1800_3_10%"      "14400_1_10%"     "14400_2_10%"     "14400_3_10%"    
+##  [13] "30_1_15%"        "30_2_15%"        "30_3_15%"        "240_1_15%"      
+##  [17] "240_2_15%"       "240_3_15%"       "1800_1_15%"      "1800_2_15%"     
+##  [21] "1800_3_15%"      "14400_1_15%"     "14400_2_15%"     "14400_3_15%"    
+##  [25] "30_1_20%"        "30_2_20%"        "30_3_20%"        "240_1_20%"      
+##  [29] "240_2_20%"       "240_3_20%"       "1800_1_20%"      "1800_2_20%"     
+##  [33] "1800_3_20%"      "14400_1_20%"     "14400_2_20%"     "14400_3_20%"    
+##  [37] "30_1_25%"        "30_2_25%"        "30_3_25%"        "240_1_25%"      
+##  [41] "240_2_25%"       "240_3_25%"       "1800_1_25%"      "1800_2_25%"     
+##  [45] "1800_3_25%"      "14400_1_25%"     "14400_2_25%"     "14400_3_25%"    
+##  [49] "30_1_5%"         "30_2_5%"         "30_3_5%"         "240_1_5%"       
+##  [53] "240_2_5%"        "240_3_5%"        "1800_1_5%"       "1800_2_5%"      
+##  [57] "1800_3_5%"       "14400_1_5%"      "14400_2_5%"      "14400_3_5%"     
+##  [61] "30_1_W169G"      "30_2_W169G"      "30_3_W169G"      "240_1_W169G"    
+##  [65] "240_2_W169G"     "240_3_W169G"     "1800_1_W169G"    "1800_2_W169G"   
+##  [69] "1800_3_W169G"    "14400_1_W169G"   "14400_2_W169G"   "14400_3_W169G"  
+##  [73] "30_1_WT Null"    "30_2_WT Null"    "30_3_WT Null"    "30_4_WT Null"   
+##  [77] "30_5_WT Null"    "30_6_WT Null"    "30_7_WT Null"    "240_1_WT Null"  
+##  [81] "240_2_WT Null"   "240_3_WT Null"   "240_4_WT Null"   "240_5_WT Null"  
+##  [85] "240_6_WT Null"   "240_7_WT Null"   "1800_1_WT Null"  "1800_2_WT Null" 
+##  [89] "1800_3_WT Null"  "1800_4_WT Null"  "1800_5_WT Null"  "1800_6_WT Null" 
+##  [93] "1800_7_WT Null"  "14400_1_WT Null" "14400_2_WT Null" "14400_3_WT Null"
+##  [97] "14400_4_WT Null" "14400_5_WT Null" "14400_6_WT Null" "14400_7_WT Null"
+
new.colnames <- gsub("0_", "0rep", paste0("X", colnames(MBP_wide)[-c(1,2)]))
+new.colnames <- gsub("_", "cond", new.colnames)
+
+# remove annoying % signs
+new.colnames <- gsub("%", "", new.colnames)
+
+# remove space (NULL could get confusing later and WT is clear)
+new.colnames <- gsub(" .*", "", new.colnames)
+
+new.colnames
+
##   [1] "X30rep1cond10"       "X30rep2cond10"       "X30rep3cond10"      
+##   [4] "X240rep1cond10"      "X240rep2cond10"      "X240rep3cond10"     
+##   [7] "X1800rep1cond10"     "X1800rep2cond10"     "X1800rep3cond10"    
+##  [10] "X14400rep1cond10"    "X14400rep2cond10"    "X14400rep3cond10"   
+##  [13] "X30rep1cond15"       "X30rep2cond15"       "X30rep3cond15"      
+##  [16] "X240rep1cond15"      "X240rep2cond15"      "X240rep3cond15"     
+##  [19] "X1800rep1cond15"     "X1800rep2cond15"     "X1800rep3cond15"    
+##  [22] "X14400rep1cond15"    "X14400rep2cond15"    "X14400rep3cond15"   
+##  [25] "X30rep1cond20"       "X30rep2cond20"       "X30rep3cond20"      
+##  [28] "X240rep1cond20"      "X240rep2cond20"      "X240rep3cond20"     
+##  [31] "X1800rep1cond20"     "X1800rep2cond20"     "X1800rep3cond20"    
+##  [34] "X14400rep1cond20"    "X14400rep2cond20"    "X14400rep3cond20"   
+##  [37] "X30rep1cond25"       "X30rep2cond25"       "X30rep3cond25"      
+##  [40] "X240rep1cond25"      "X240rep2cond25"      "X240rep3cond25"     
+##  [43] "X1800rep1cond25"     "X1800rep2cond25"     "X1800rep3cond25"    
+##  [46] "X14400rep1cond25"    "X14400rep2cond25"    "X14400rep3cond25"   
+##  [49] "X30rep1cond5"        "X30rep2cond5"        "X30rep3cond5"       
+##  [52] "X240rep1cond5"       "X240rep2cond5"       "X240rep3cond5"      
+##  [55] "X1800rep1cond5"      "X1800rep2cond5"      "X1800rep3cond5"     
+##  [58] "X14400rep1cond5"     "X14400rep2cond5"     "X14400rep3cond5"    
+##  [61] "X30rep1condW169G"    "X30rep2condW169G"    "X30rep3condW169G"   
+##  [64] "X240rep1condW169G"   "X240rep2condW169G"   "X240rep3condW169G"  
+##  [67] "X1800rep1condW169G"  "X1800rep2condW169G"  "X1800rep3condW169G" 
+##  [70] "X14400rep1condW169G" "X14400rep2condW169G" "X14400rep3condW169G"
+##  [73] "X30rep1condWT"       "X30rep2condWT"       "X30rep3condWT"      
+##  [76] "X30rep4condWT"       "X30rep5condWT"       "X30rep6condWT"      
+##  [79] "X30rep7condWT"       "X240rep1condWT"      "X240rep2condWT"     
+##  [82] "X240rep3condWT"      "X240rep4condWT"      "X240rep5condWT"     
+##  [85] "X240rep6condWT"      "X240rep7condWT"      "X1800rep1condWT"    
+##  [88] "X1800rep2condWT"     "X1800rep3condWT"     "X1800rep4condWT"    
+##  [91] "X1800rep5condWT"     "X1800rep6condWT"     "X1800rep7condWT"    
+##  [94] "X14400rep1condWT"    "X14400rep2condWT"    "X14400rep3condWT"   
+##  [97] "X14400rep4condWT"    "X14400rep5condWT"    "X14400rep6condWT"   
+## [100] "X14400rep7condWT"
+

We will now parse the data into an object of class QFeatures, we have provided +a function to assist with this in the package. If you want to do this yourself +use the readQFeatures function from the QFeatures package.

+
MBPqDF <- parseDeutData(object = DataFrame(MBP_wide),
+                        design = new.colnames,
+                        quantcol = 3:102)
+
+
+

5 Heatmap visualisations of HDX data

+

To help us get used to the QFeatures we show how to generate a heatmap +of these data from this object:

+
pheatmap(t(assay(MBPqDF)),
+         cluster_rows = FALSE, 
+         cluster_cols = FALSE,
+         color = brewer.pal(n = 9, name = "BuPu"),
+         main = "Stuctural variant deuterium incoperation heatmap", 
+         fontsize = 14,
+         legend_breaks = c(0, 2, 4, 6, 8, 10, 12, max(assay(MBPqDF))),
+         legend_labels = c("0", "2", "4", "6", "8","10", "12", "Incorporation"))
+

+If you prefer to have the start-to-end residue numbers in the heatmap instead +you can change the plot as follows:

+
regions <- unique(MBP[,c("pep_start", "pep_end")])
+xannot <- paste0("[", regions[,1], ",", regions[,2], "]")
+pheatmap(t(assay(MBPqDF)),
+         cluster_rows = FALSE, 
+         cluster_cols = FALSE,
+         color = brewer.pal(n = 9, name = "BuPu"),
+         main = "Stuctural variant deuterium incoperation heatmap", 
+         fontsize = 14,
+         legend_breaks = c(0, 2, 4, 6, 8, 10, 12, max(assay(MBPqDF))),
+         legend_labels = c("0", "2", "4", "6", "8","10", "12", "Incorporation"),
+         labels_col = xannot)
+

+It maybe useful to normalize HDX-MS data for either interpretation or +visualization purposes. We can normalize by the number of exchangeable amides +or by using back-exchange correction values. We first use percentage +incorporation as normalisation and visualise as a heatmap.

+
MBPqDF_norm1 <- normalisehdx(MBPqDF,
+                             sequence = unique(MBP$pep_sequence),
+                             method = "pc")
+
+
+pheatmap(t(assay(MBPqDF_norm1)),
+         cluster_rows = FALSE, 
+         cluster_cols = FALSE,
+         color = brewer.pal(n = 9, name = "BuPu"),
+         main = "Stuctural variant deuterium incoperation heatmap normalised", 
+         fontsize = 14,
+         legend_breaks = c(0, 0.2, 0.4, 0.6, 0.8, 1, 1.2),
+         legend_labels = c("0", "0.2", "0.4", "0.6", "0.8","1", "Incorporation"),
+         labels_col = xannot)
+

+Now, we demonstrate a back-exchange correction calculation. The +back-exchange value are fictious by the code chunk below demonstrates how +to set this up.

+
# made-up correction factor
+correction <- (exchangeableAmides(unique(MBP$pep_sequence)) + 1) * 0.9
+
+MBPqDF_norm2 <- normalisehdx(MBPqDF,
+                             sequence = unique(MBP$pep_sequence),
+                             method = "bc", 
+                             correction = correction)
+
+
+pheatmap(t(assay(MBPqDF_norm2)),
+         cluster_rows = FALSE, 
+         cluster_cols = FALSE,
+         color = brewer.pal(n = 9, name = "BuPu"),
+         main = "Stuctural variant deuterium incoperation heatmap normalised", 
+         fontsize = 14,
+         legend_breaks = c(0, 0.2, 0.4, 0.6, 0.8, 1, 1.2),
+         legend_labels = c("0", "0.2", "0.4", "0.6", "0.8","1", "Incorporation"),
+         labels_col = xannot)
+

+
+
+

6 Functional data analysis of HDX-MS data

+

The hdxstats package uses an empirical Bayes functional approach to analyse +the data. We explain this idea in steps so that we can get an idea of the approach. +First we fit the parametric model to the data. This will allow us to explore +the HdxStatModel class.

+
res <- differentialUptakeKinetics(object = MBPqDF[,1:100], #provide a QFeature object
+                                  feature = rownames(MBPqDF)[[1]][37], # which peptide to do we fit
+                                  start = list(a = NULL, b = 0.0001,  d = NULL, p = 1)) # what are the starting parameter guesses
+
## Warning in differentialUptakeKinetics(object = MBPqDF[, 1:100], feature =
+## rownames(MBPqDF)[[1]][37], : NAs introduced by coercion
+

Here, we see the HdxStatModel class, and that a Functional Model was applied +to the data and a total of 7 models were fitted.

+
res
+
## Object of class "HdxStatModel"
+## Method: Functional Model 
+## Fitted 7
+

The nullmodel and alternative slots of an instance of HdxStatModel provide +the underlying fitted models. The method and formula slots provide vital +information about what analysis was performed. The vis slot provides a ggplot +object so that we can visualise the functional fits.

+
res@vis
+

+We can also explore other functional model rather than the weibull for example +a model that is the sum of two logistic curves.

+
res2 <- differentialUptakeKinetics(object = MBPqDF[,1:100], #provide a QFeature object,
+                                  formula = value ~ a*(1 - exp(-b * timepoint)) + c * (1 - exp(-d * timepoint)),
+                                  feature = rownames(MBPqDF)[[1]][37], # which peptide to do we fit
+                                  start = list(a = 0, b = 0.0001,  c = 0, d = 0.0001)) # what are the starting parameter guesses
+
## Warning in differentialUptakeKinetics(object = MBPqDF[, 1:100], formula = value
+## ~ : NAs introduced by coercion
+

We can then visualize the output of our curve fitting

+
res2@vis
+

+

one question would be can we statistically say whether one model is better +than another. We can do this by computing the likelihood ratio between the two +proposed models. We can see the likelihoods from the second model are generally +higher.

+
logLik(res)
+
##       null       alt1       alt2       alt3       alt4       alt5       alt6 
+## -43.887971  13.126180  11.888973  14.550056  16.637855  12.387702   9.762426 
+##       alt7 
+##  29.610175
+
logLik(res2)
+
##      null      alt1      alt2      alt3      alt4      alt5      alt6      alt7 
+## -41.95843  23.54976  22.10180  25.32149  23.77991  22.07919  26.54933  52.04347
+

Let compute minus twice the difference of the log-likelihoods:

+
diff <- -2 * (logLik(res) - logLik(res2))
+

Wilk’s theorem tell us that this statistics is asymptotically distributed as +chi-squared with degree of freedom equal to the difference of the degrees +of freedom of each model. Each model has 4 degrees of freedom so in this +case there is no need to apply any theoretical results. The second model is +preferred, as it as higher log-likelihood

+
+ + + +
+
+ +
+ + + + + + + + + + + + + + + + + From 6c6acb8d5cb6b1d6b55c5f159b0aa08a01f7a969 Mon Sep 17 00:00:00 2001 From: Broncio Aguilar Sanjuan Date: Thu, 1 Sep 2022 15:14:42 +0100 Subject: [PATCH 07/12] swapped print with return in foresplot function --- R/functionalAnalysis-plots.R | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/R/functionalAnalysis-plots.R b/R/functionalAnalysis-plots.R index 6ac07a8..88355f5 100644 --- a/R/functionalAnalysis-plots.R +++ b/R/functionalAnalysis-plots.R @@ -729,7 +729,7 @@ forestPlot <- function(params, condition = c(1,2)) { scale_color_viridis(discrete = TRUE, begin = 0.2, direction = 1) + theme(text = element_text(size = 16)) + geom_vline(xintercept = 0, linetype = "dashed") - print(gg) + return(gg) } ##' Underlying computation for compute residue level differences plots. Uses ##' average differences at each residue. From 20d69ce4912293bee26a74598386cb7f6c1c5e05 Mon Sep 17 00:00:00 2001 From: "Oliver M. Crook" Date: Thu, 15 Jan 2026 12:39:10 +0000 Subject: [PATCH 08/12] Update hdxstat-class.R --- R/hdxstat-class.R | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/R/hdxstat-class.R b/R/hdxstat-class.R index ce2d45e..7f25047 100644 --- a/R/hdxstat-class.R +++ b/R/hdxstat-class.R @@ -32,7 +32,7 @@ setOldClass("gg") .hdxstatmodel <- setClass("HdxStatModel", slots = c(nullmodel = "nls", alternative = "nlsList", - vis = "gg", + vis = "ggplot", method = "character", formula = "formula"), validity = function(object){ From a8de7aecda71b7ba0bb1832855cec3bd01b0e3b9 Mon Sep 17 00:00:00 2001 From: "Oliver M. Crook" Date: Thu, 15 Jan 2026 13:44:24 +0000 Subject: [PATCH 09/12] Update hdxstat-class.R --- R/hdxstat-class.R | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/R/hdxstat-class.R b/R/hdxstat-class.R index 7f25047..ce2d45e 100644 --- a/R/hdxstat-class.R +++ b/R/hdxstat-class.R @@ -32,7 +32,7 @@ setOldClass("gg") .hdxstatmodel <- setClass("HdxStatModel", slots = c(nullmodel = "nls", alternative = "nlsList", - vis = "ggplot", + vis = "gg", method = "character", formula = "formula"), validity = function(object){ From 457f63a587ecf057a1e18e20b102d9847b071be6 Mon Sep 17 00:00:00 2001 From: "Oliver M. Crook" Date: Thu, 15 Jan 2026 14:07:20 +0000 Subject: [PATCH 10/12] Change vis slot type to ANY in HdxStatModel --- R/hdxstat-class.R | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/R/hdxstat-class.R b/R/hdxstat-class.R index ce2d45e..d50e1c9 100644 --- a/R/hdxstat-class.R +++ b/R/hdxstat-class.R @@ -32,7 +32,7 @@ setOldClass("gg") .hdxstatmodel <- setClass("HdxStatModel", slots = c(nullmodel = "nls", alternative = "nlsList", - vis = "gg", + vis = "ANY", method = "character", formula = "formula"), validity = function(object){ From c1f2400fd719ffd8635686f060e4a5c0de857011 Mon Sep 17 00:00:00 2001 From: "Oliver M. Crook" Date: Thu, 15 Jan 2026 14:18:35 +0000 Subject: [PATCH 11/12] Update DESCRIPTION --- DESCRIPTION | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/DESCRIPTION b/DESCRIPTION index 1c2d145..b0dcfac 100644 --- a/DESCRIPTION +++ b/DESCRIPTION @@ -1,6 +1,6 @@ Package: hdxstats Title: Statistical analysis of hydrogen deuterium exchange mass spectrometry -Version: 0.99.0 +Version: 0.99.1 Authors@R: c(person(family = "Crook", given = "Oliver M.", email = "oliver.crook@stats.ox.ac.uk", comment = c(ORCID = "0000-0001-5669-8506"), From d0dd4e00e6e083e561d9e07d78c1e92fd62fcf4d Mon Sep 17 00:00:00 2001 From: "Oliver M. Crook" Date: Fri, 30 Jan 2026 13:28:31 +0000 Subject: [PATCH 12/12] Bump version from 0.99.1 to 1.0.1 --- DESCRIPTION | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/DESCRIPTION b/DESCRIPTION index b0dcfac..860bf38 100644 --- a/DESCRIPTION +++ b/DESCRIPTION @@ -1,6 +1,6 @@ Package: hdxstats Title: Statistical analysis of hydrogen deuterium exchange mass spectrometry -Version: 0.99.1 +Version: 1.0.1 Authors@R: c(person(family = "Crook", given = "Oliver M.", email = "oliver.crook@stats.ox.ac.uk", comment = c(ORCID = "0000-0001-5669-8506"),