Running guppy on ecoli benchmark

[ardakdemir]

This is not a issue related to this github repository, but I wonder if you also had a similar error when basecalling on the Ecoli benchmark fast5 files:

Fast5 read file is invalid. Read is missing 'median_before' metadata.

Other basecallers work fine with your provided scripts yet, for these specific fast5 files guppy does not work because of the missing metadata. Is there a way to side-step this problem, or should I add the metadata manually inside the fast5 files?

[cameron-jack]

We've got this issue too. Does anyone have any insights as to how to fix/get-around it?

[Bessyyi]

This is not a issue related to this github repository, but I wonder if you also had a similar error when basecalling on the Ecoli benchmark fast5 files:

Fast5 read file is invalid. Read is missing 'median_before' metadata.

Other basecallers work fine with your provided scripts yet, for these specific fast5 files guppy does not work because of the missing metadata. Is there a way to side-step this problem, or should I add the metadata manually inside the fast5 files?

hello，professor. I got this issue too.Did you have solved this problem?