failed to use cell volume as latent_var

[ynpan910]

Hi,

I'm analyzing spatial transcriptomics data. Instead of using the default log_umi as the latent_var, I would like to regress out cell volume. I have put cell volume for each cell as a column in seu@meta.data. However, I run the below code and got error:

seu$log_cell_volume <- log1p(seu$volume)  
seu <- SCTransform(
  seu,
  assay = "RNA",
  vars.to.regress = NULL,          
  latent_var = "log_cell_volume",  
  verbose = TRUE
)

error in h(simpleError(msg, call)) : 
  error in evaluating the argument 'x' in selecting a method for function 'as.matrix': undefined columns selected

The error should come from the step of Getting residuals for block 1(of 14) for counts dataset.

I have no NA in the log_cell_volume column.

Can you suggest if this is doable with SCT, and if so, how to correctly do it?

Thanks for any help!