Skip to content

pygor3 Aborted : returned non-zero exit status 134. #5

@niccolocard99

Description

@niccolocard99

Hello

I work on WSL with ubuntu 20.04

I belive I have installed Igor and pygor3 successfully (downloaded from github, then ./configure && make && sudo make install ),
and commands like
pygor imgt-get-genomes --imgt-species Homo+sapiens --imgt-chain IGH -t VDJ
or
pygor model-create -M models/Homo+sapiens/IGH/ -t VDJ
run smoothly without errors.

but when i try to run pygor igor-infer -M models/Homo+sapiens/IGH/ -i ./Igor_testo5.txt -o new_IgHHHH

stops after the alignment (Aborted)
and then returns returned non-zero exit status 134.

I Also tried the same commands on jupyternotebooks , but the outcome is the same

Below is the output from my console:

$ pygor igor-infer -M models/Homo+sapiens/IGH/ -i ./Igor_testo5.txt -o new_IgHHHH
--------------------------------
===== Running inference =====
Reading Parms filename from:  models/Homo+sapiens/IGH//models/model_parms.txt
Reading Marginals filename from:  models/Homo+sapiens/IGH//models/model_marginals.txt
/usr/local/bin/igor -set_wd ./ -batch dataIGoRjb9x8CHN78 -read_seqs ./Igor_testo5.txt
/usr/local/bin/igor -set_wd ./ -batch dataIGoRjb9x8CHN78 -set_genomic  --V models/Homo+sapiens/IGH//ref_genome//genomicVs.fasta --D models/Homo+sapiens/IGH//ref_genome//genomicDs.fasta --J models/Homo+sapiens/IGH//ref_genome//genomicJs.fasta -set_CDR3_anchors  --V models/Homo+sapiens/IGH//ref_genome//V_gene_CDR3_anchors.csv --J models/Homo+sapiens/IGH//ref_genome//J_gene_CDR3_anchors.csv -align  --all
Batch name set to: dataIGoRjb9x8CHN78_
Working directory set to: "./"
Performing V alignments....
V_gene alignments [||||||||||||||||||||||||||||||||||||||||||||||||||]  Done.
Performing D alignments....
D_gene alignments [||||||||||||||||||||||||||||||||||||||||||||||||||]  Done.
Performing J alignments....
J_gene alignments [||||||||||||||||||||||||||||||||||||||||||||||||||]  Done.
Performing CDR3 sequence extraction ....
== Alignment finished! ==
Writing model parms in file  .//dataIGoRjb9x8CHN78_mdldata//models/model_parms.txt
Writing model marginals in file  .//dataIGoRjb9x8CHN78_mdldata//models/model_marginals.txt
/usr/local/bin/igor -set_wd ./ -batch dataIGoRjb9x8CHN78 -set_custom_model .//dataIGoRjb9x8CHN78_mdldata//models/model_parms.txt .//dataIGoRjb9x8CHN78_mdldata//models/model_marginals.txt -set_CDR3_anchors  --V .//dataIGoRjb9x8CHN78_mdldata//ref_genome/V_gene_CDR3_anchors.csv --J .//dataIGoRjb9x8CHN78_mdldata//ref_genome/J_gene_CDR3_anchors.csv -infer
Batch name set to: dataIGoRjb9x8CHN78_
GeneChoice read
GeneChoice read
GeneChoice read
Deletion read
Deletion read
Deletion read
Deletion read
Insertion read
DinucMarkov read
Aborted
Traceback (most recent call last):
  File "/home/nick99/.local/bin/pygor", line 8, in <module>
    sys.exit(cli())
  File "/usr/lib/python3/dist-packages/click/core.py", line 764, in __call__
    return self.main(*args, **kwargs)
  File "/usr/lib/python3/dist-packages/click/core.py", line 717, in main
    rv = self.invoke(ctx)
  File "/usr/lib/python3/dist-packages/click/core.py", line 1137, in invoke
    return _process_result(sub_ctx.command.invoke(sub_ctx))
  File "/usr/lib/python3/dist-packages/click/core.py", line 956, in invoke
    return ctx.invoke(self.callback, **ctx.params)
  File "/usr/lib/python3/dist-packages/click/core.py", line 555, in invoke
    return callback(*args, **kwargs)
  File "/home/nick99/.local/lib/python3.8/site-packages/pygor3/scripts/cli.py", line 341, in run_infer
    output = igortask._run_infer(igor_read_seqs=igor_read_seqs)
  File "/home/nick99/.local/lib/python3.8/site-packages/pygor3/IgorIO.py", line 7535, in _run_infer
    raise e
  File "/home/nick99/.local/lib/python3.8/site-packages/pygor3/IgorIO.py", line 7528, in _run_infer
    output = run_command_print(cmd)
  File "/home/nick99/.local/lib/python3.8/site-packages/pygor3/IgorIO.py", line 127, in run_command_print
    raise e
  File "/home/nick99/.local/lib/python3.8/site-packages/pygor3/IgorIO.py", line 121, in run_command_print
    for path in execute_command_generator(cmd):
  File "/home/nick99/.local/lib/python3.8/site-packages/pygor3/IgorIO.py", line 115, in execute_command_generator
    raise subprocess.CalledProcessError(return_code, cmd)
subprocess.CalledProcessError: Command '/usr/local/bin/igor -set_wd ./ -batch dataIGoRjb9x8CHN78 -set_custom_model .//dataIGoRjb9x8CHN78_mdldata//models/model_parms.txt .//dataIGoRjb9x8CHN78_mdldata//models/model_marginals.txt -set_CDR3_anchors 
--V .//dataIGoRjb9x8CHN78_mdldata//ref_genome/V_gene_CDR3_anchors.csv
 --J .//dataIGoRjb9x8CHN78_mdldata//ref_genome/J_gene_CDR3_anchors.csv -infer  ' returned non-zero exit status 134.

Can anybody help?
Thanks

Niccolò

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions