What's the purpose of this construct?
Integrase expression construct. Primary goal is to establish an efficient protocol for sequential insertion of DNA fragments into a backbone for (1) bar-coding purposes and (2) combinatorial pathway assembly.
Discussion & Comments
Judging from literature BxB1 is, so far, the best-performing Serine recombinase for cloning purposes. It features fast kinetics and near 100% recombination efficiency without any excision activity.
Implementation Progress
What's the purpose of this construct?
Integrase expression construct. Primary goal is to establish an efficient protocol for sequential insertion of DNA fragments into a backbone for (1) bar-coding purposes and (2) combinatorial pathway assembly.
Discussion & Comments
Judging from literature BxB1 is, so far, the best-performing Serine recombinase for cloning purposes. It features fast kinetics and near 100% recombination efficiency without any excision activity.
Implementation Progress