Hi,
Recently I used TelSeq to estimate telomere length from WGS sequencing data (NovaSeq), with k = 7 and r = 151. The returned length falls in the range of 0.4 - 0.5kb. However for a different batch of samples (HiSeq) with the same parameters, the returned length is from 5kb to 15kb.
When I checked the output files, I could see the amount of TEL0 - TEL1 - TEL2 reads were much higher in NovaSeq samples compared to HiSeq samples (also the mount of TEL10+ was lower in NovaSeq samples). I troubleshooted by reducing k to 4, which helped increase the length to 1.4kb, but this is still not the normal range of telomere length.
May I know if you have experience with running NovaSeq samples before, and I kindly seek for recommendation to fix this short telomere length issue. Thank you very much! Your help is greatly appreciated.
Hi,
Recently I used TelSeq to estimate telomere length from WGS sequencing data (NovaSeq), with k = 7 and r = 151. The returned length falls in the range of 0.4 - 0.5kb. However for a different batch of samples (HiSeq) with the same parameters, the returned length is from 5kb to 15kb.
When I checked the output files, I could see the amount of TEL0 - TEL1 - TEL2 reads were much higher in NovaSeq samples compared to HiSeq samples (also the mount of TEL10+ was lower in NovaSeq samples). I troubleshooted by reducing k to 4, which helped increase the length to 1.4kb, but this is still not the normal range of telomere length.
May I know if you have experience with running NovaSeq samples before, and I kindly seek for recommendation to fix this short telomere length issue. Thank you very much! Your help is greatly appreciated.