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These packages combine Nanopore data with high throughput expression data. It has been used on 16 mtPlates combined with Nanopore FastQ files. Merger_blast.py requires a BLAST+ library containing the gene of interest to run as well as the BLAST+ software.

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AskeU/Protein_expression_data

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Protein_expression_data repository description

Script description

This is the initial code for the article: "Application of long-read sequencing to establish a cost-efficient high-throughput automated pipeline for generation of organism specific protein-expression data" The Lead_script.py runs the Merger_blast.py, Double_barcode_Demultiplexer_vectorized.py and Demultiplexed_To_Stuctured_With_Lum_data.py in order to transform Nanopore FastQ files and Excel-sheets from out Hamilton robot into structured data and plots.

Fw/rev primers are the primers containing barcodes and the independent scripts Hamilton_Data_Extractor(Individual).py and Clustered_luminescence.py visualizes the data from the Hamilton alone and from the output of the Lead_script.py.

The scripts can still be improved but they work consistently with 16 mtplates at a time. They will be improved later.

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These packages combine Nanopore data with high throughput expression data. It has been used on 16 mtPlates combined with Nanopore FastQ files. Merger_blast.py requires a BLAST+ library containing the gene of interest to run as well as the BLAST+ software.

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