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4 changes: 2 additions & 2 deletions README.md
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# BioHackAcademy.github.io
Waag Society's BioHack Academy Syllabus
# BioHackAcademyPTBR.github.io
Ementa do BioHack Academy da Waag Society, em bom português!
7 changes: 4 additions & 3 deletions _config.yml
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# Site settings
title: Biohack Academy Syllabus
email: pieter@waag.org
title: Ementa Biohack Academy
email: info@garagemfablab.com
description: > # this means to ignore newlines until "baseurl:"
Waag Society's first Biohack Academy is an intense 10 week bioreactor hacking course held in the Amsterdam Fablab and Open Wetlab.
O primeiro BioHack Academy da Waag Society's é um curso intenso de 10 semanas sobre como construir e hackaear um biorreator com ferramentas de fabricação digital, conhecimento aberto e espírito DIY!
Será ministrado no Open WetLab do FabLab Amsterdam e em outros FabLabs pelo mundo, como no Garagem FabLab em SP e Olabi no RJ.
baseurl: "" # the subpath of your site, e.g. /blog/
url: "http://code.waag.org" # the base hostname & protocol for your site
twitter_username: waag
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9 changes: 5 additions & 4 deletions about.md
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permalink: /about/
---

Biohack Academy is an education program of Waag Society Amsterdam. You can find more information on [waag.org/biohackacademy](http://www.waag.org/biohackacademy)
O Biohack Academy é um programa educacional da Waag Society de Amsterdam. Você pode encontrar mais infos em [waag.org/biohackacademy](http://www.waag.org/biohackacademy)

![Waag Society Open Wetlab]({{ site.baseurl }}/biofactory/graphics/WS_openwetlab_logo.png)

This site was made using the base Jekyll theme. You can find out more info about customizing this Jekyll theme, as well as basic Jekyll usage documentation at [jekyllrb.com](http://jekyllrb.com/)
Este site foi criado usando como base o tema Jekyll. Você pode encontrar mais informações sobre como customizar esse tema, assim como a documentação básica sobre seu uso em [jekyllrb.com](http://jekyllrb.com/)

You can find the source code for the Jekyll new theme at: [github.com/jglovier/jekyll-new](https://github.com/jglovier/jekyll-new)
Aqui você encontra o código-fonte do Jekyll New Theme:
[github.com/jglovier/jekyll-new](https://github.com/jglovier/jekyll-new)

You can find the source code for Jekyll at [github.com/jekyll/jekyll](https://github.com/jekyll/jekyll)
E aqui você encontra o código-fonte do Jekyll [github.com/jekyll/jekyll](https://github.com/jekyll/jekyll)
88 changes: 44 additions & 44 deletions biofactory/class/1-incubator.md
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---
layout: page
title: Incubator (Class 1)
title: Incubadora (Class 1)
permalink: /biofactory/class/1-incubator/
---

## Synopsys
## Sinopse

Build your own incubator and start growing microbes under controlled conditions. During the class you will also get to know the fundamentals of microbiology. We’ll use our purple pigment bacteria as a standard, but you can also choose your own favourite biological agent. A crash course Arduino programming and soldering is included.
Construa sua própria incubadora e comece a cultivar seus próprios microorganismos sob condições controladas. Durante as aulas você vai conhecer também os fundamentos da microbiologia. Vamos usar um pigmento roxo padrão para vizualisar as bactérias, mas você também pode optar por seu agente biológico favorito. Um curso intensivo de programação Arduino e soldagem está incluso.

## Schedule
## Agenda

* Introduction
* Microbiology fundamentals & Biological safety instructions
* Arduino programming & soldering
* Incubator design
* Documentating your designs
* Microorganism cultivation & enrichment practical intro
* [Contamination test](/biofactory/class/1-incubator/contamination-test/)
* [Isolation of Yoghurt bacteria](/biofactory/class/1-incubator/yoghurt-bacteria-isolation/)
* [Isolation of Beer yeasts](/biofactory/class/1-incubator/beer-yeasts-isolation/)
* Tour around your lab
* Introdução
* Fundamentos da microbiologia & instruções de segurança biológica
* Programação Arduino & soldagem
* Projeto da incubadora
* Documentação dos projetos
* Introdução prática ao cultivo & enriquecimento de microorganismos
* [Teste de contaminação](/biofactory/class/1-incubator/contamination-test/)
* [Isolamento de bactérias do iogurte](/biofactory/class/1-incubator/yoghurt-bacteria-isolation/)
* [Isolamento de leveduras da cerveja](/biofactory/class/1-incubator/beer-yeasts-isolation/)
* Tour do laboratório

## Incubator Design
## Projeto da incubadora

This week we will build our first Open Hardware lab device: the incubator. Download the design files here:
Essa semana nós vamos construir nosso primeiro dispositivo de laboratório Open Hardware. Baixe os arquivos do projeto aqui:

* [Functional and technical requirements](/biofactory/class/1-incubator/requirements/)
* [SketchUp assembly](/biofactory/class/1/Incubator-Sketchup.skp)
* [SVG laser cut files](/biofactory/class/1/Incubator-SVGs.zip)
* [Fritzing wiring](/biofactory/class/1/Incubator-Fritzing.fzz)
* [Arduino code](/biofactory/class/1/Incubator-Arduino.txt)
* [Requisitos funcionais e técnicos](/biofactory/class/1-incubator/requirements/)
* [Montagem do SketchUp](/biofactory/class/1/Incubator-Sketchup.skp)
* [Arquivos SVG para corte a laser](/biofactory/class/1/Incubator-SVGs.zip)
* [Diagrama Fritzing](/biofactory/class/1/Incubator-Fritzing.fzz)
* [Código Arduino](/biofactory/class/1/Incubator-Arduino.txt)

![Incubator](/biofactory/class/1/Incubator.png)
![Incubadora](/biofactory/class/1/Incubator.png)

## Bill of Materials
## Lista de Materiais

In order to take part in this class you will need the following materials:
Para fazer parte dessa aula, você precisará dos seguintes materiais:

* [Incubator materials](/biofactory/class/1-incubator/incubator-materials/)
* [Microbiology practical materials](/biofactory/class/1-incubator/microbiology-materials/)
* [Materiais da incubadora](/biofactory/class/1-incubator/incubator-materials/)
* [Materiais para a prática da microbiologia](/biofactory/class/1-incubator/microbiology-materials/)

## Assignment
## Tarefa

Set up your own documentation site on Github.
Organize sua própria pasta de documentos aqui no Github.

## Additional reading and hacking
## Leituras e hacking adicionais

* Microbiology
* [Microbiology](http://education-portal.com/academy/course/microbiology-course.html)
* [Introduction to Microbiology Culture Techniques](https://www.youtube.com/watch?v=Et1v8EQP10U) YouTube video
* Microbiologia
* [Microbiologia](http://education-portal.com/academy/course/microbiology-course.html)
* [Introdução às Técnicas de Cultivo de Microbiologia](https://www.youtube.com/watch?v=Et1v8EQP10U)
* Arduino
* [Getting started with Arduino](http://www.makeuseof.com/tag/getting-started-with-arduino-a-beginners-guide/)
* [Ladyada Arduino Tutorials](http://www.ladyada.net/learn/arduino/)
* [Soldering](https://www.youtube.com/watch?v=oqV2xU1fee8) YouTube video
* Incubators
* [Começando com Arduino](http://www.makeuseof.com/tag/getting-started-with-arduino-a-beginners-guide/)
* [Tutoriais Ladyada Arduino ](http://www.ladyada.net/learn/arduino/)
* [Soldagem](https://www.youtube.com/watch?v=oqV2xU1fee8)
* Incubadoras
* [Climaduino](http://www.instructables.com/id/Introducing-Climaduino-The-Arduino-Based-Thermosta/)
* [Biodesign DIY Incubator](http://biodesign.cc/2013/12/25/diy-incubator/)
* [Bioprojeto DIY Incubadora](http://biodesign.cc/2013/12/25/diy-incubator/)
* Github
* [How the heck do I use Github](http://lifehacker.com/5983680/how-the-heck-do-i-use-github)
* [Como raios eu uso o Github?](http://lifehacker.com/5983680/how-the-heck-do-i-use-github)
* [Git cheatsheet](http://rogerdudler.github.io/git-guide/)
* Additional Practicals
* [Isolation of Bioluminescent bacteria](/biofactory/class/1-incubator/bioluminescent-bacteria-isolation/)
* [Isolation of Nitrogen-fixating bacteria](/biofactory/class/1-incubator/isolation-of-nitrogen-fixating-bacteria/)
* [Isolation of Sulphur-oxidizing bacteria](/biofactory/class/1-incubator/isolation-of-sulphur-oxidizing-bacteria/)
* Práticas Adicionais
* [Isolamento de bactérias bioluminescentes](/biofactory/class/1-incubator/bioluminescent-bacteria-isolation/)
* [Isolamento de bactérias fixadoras de Nitrogênio](/biofactory/class/1-incubator/isolation-of-nitrogen-fixating-bacteria/)
* [Isolamento de bactérias oxidantes de Enxofre](/biofactory/class/1-incubator/isolation-of-sulphur-oxidizing-bacteria/)

## Feeling lazy?
## Está com preguiça?

Go ahead and buy an incubator:
Vá em frente e compre uma incubadora:

* [Lucky Reptile](http://www.amazon.co.uk/Lucky-Reptile-HN-2UK-Nursery-Incubator/dp/B002NFR0HQ)
24 changes: 12 additions & 12 deletions biofactory/class/1/contamination-test.md
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Expand Up @@ -4,21 +4,21 @@ title: Contamination Test
permalink: /biofactory/class/1-incubator/contamination-test/
---

## Goal
## Objetivo

Microorganisms are always around and ready to contaminate your carefully grown cultures. In this practical we will determine what common airborne contaminents look like.
Os microorganismos estão sempre por perto e prontos para contaminar as suas culturas cuidadosamente cultivadas. Através deste teste, você vai aprender como surgem os contaminantes transportados pelo ar mais comuns.

## Materials
## Materiais

* 1 Peptone agar (PA) plate, favourable to bacteria
* 1 Malt agar (MA) plate, favourable to yeast and fungi
* 1 placa de Peptone Agar (PA), favorável às bactérias
* 1 placa de Agar de malte (MA), favorável à leveduras e fungos

## Method
## Metodologia

1. Find an interesting spot in the building.
2. Remove the lid from the plates and leave the plates for 30 minutes.
3. Collect the plates.
4. Incubate at 30° C for 24h.
5. Inspect your plates and look at them under the microscope.
1. Ache um local interessante no seu entorno. Pode ser uma sala, um banheiro…
2. Remova a tampa das placas e deixe as placas em repouso durante 30 minutos.
3. Recolha as placas.
4. Incube-as a 30°C durante 24h.
5. Inspecione suas placas e olhe-as com um microscópio.

Back to [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/)
De volta ao [Biofactory - Class 1: Incubator](/biofactory/class/1-incubator/)
41 changes: 20 additions & 21 deletions biofactory/class/1/incubator-materials.md
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---
layout: page
title: Incubator Materials
title: Materiais Incubadora
permalink: /biofactory/class/1-incubator/incubator-materials/
---

> PLEASE NOTE: as this is the first Bill of Materials of the Academy take note of the following:
> CONSIDERAÇÕES IMPORTANTES: como essa é a primeira BOM (Bill of Materials ou Lista de Materiais) do Curso, preste atenção ao seguinte:

1. The components on this list are the bare minimum you need to build an incubator and compatible with the incubator [3D design](/biofactory/class/1/Incubator-Sketchup.skp), [Fritzing wiring](/biofactory/class/1/Incubator-Fritzing.fzz) and [Arduino code](/biofactory/class/1/Incubator-Arduino.txt). Please feel free to deviate from this list and change the design accordingly. Once the classes started we will keep track of your hacks on your own documentation site.
2. The suppliers all ship to addresses in The Netherlands, and some also ship abroad. Alternative sources are listed on the [Suppliers](/suppliers/) page. Whenever you find a suitable alternative, please post it in the comments at the bottom of the page.
3. Check the stock level and shipping times of each component before ordering. If it takes more than two weeks, look for an alternative.
4. Some suppliers, such as Farnell, only ship to businesses by default. Contact their service department to order as an individual.
1. Os componentes dessa lista são o mínimo que você precisará para construir uma incubadora e que são compatíveis com o presente [Projeto 3D](/biofactory/class/1/Incubator-Sketchup.skp), [Diagrama Fritzing](/biofactory/class/1/Incubator-Fritzing.fzz) e o [código em Arduino](/biofactory/class/1/Incubator-Arduino.txt). Por favor sinta-se livre para fugir dessa lista e mudar seus desenhos e planos. Uma vez que as aulas começem, nós teremos o controle de seus "hacks" nas páginas de documentação.
2. Os fornecedores dessa lista enviam para todo o Brasil. Se você achar alguma alternativa melhor ou mais barata, por favor poste-a na área de comentários no final da página [Fornecedores](suppliers/).
3. Sempre cheque o tempo estimado de envio antes de comprar. Se não der tempo de chegar para a aula, procure alternativas.

## Bill of Materials
## Lista de Materiais

Needless to say, you will need an Arduino UNO or equivalent microcontroller. Most likely you have already bought one, as part of the [general preparations](/biofactory/preparations/).
Aqui você vai precisar de um microcontrolador Arduino UNO ou equivalente. Se você se preparou direitinho lendo a página sobre [Preparações](/biofactory/preparations/) você já está com o seu em mãos.

### Must have
### Devemos ter

These are the parts you will need to assemble the incubator:
Estas são as peças que você precisa para montar a incubadora:

|#|Amount|Description|Supplier NL|Cost|
|#|Quantidade|Descrição|Fornecedor BR|Custo|
|-:|----:|:---------|:-------|---:|
|1|1|3mm MDF 95 x 45 cm|Local wood store, like [Houthandel Schmidt](https://www.google.com/maps/dir/Waag+Society,+Nieuwmarkt,+Amsterdam,+Netherlands/Houthandel+Schmidt,+Oudezijds+Achterburgwal+53,+1012+DB+Amsterdam,+Netherlands/@52.3732195,4.8971869,17z/data=!3m1!4b1!4m13!4m12!1m5!1m1!1s0x47c609b93deae857:0xa3c3b57e66c44946!2m2!1d4.900298!2d52.372807!1m5!1m1!1s0x47c609b901ad7703:0x6d511a1e0f5be9c2!2m2!1d4.89915!2d52.373417)|5.00|
|2|1|Expanded polystyrene (EPS)|[Praxis](https://www.praxis.nl/bouwmaterialen/isolatie/isolatie/isolatieplaat-eps-60-100-x-50-x-5cm-5-stuks/5322572)|7.99|
|4|1|3mm Acrylic sheet 25 x 40 cm|[Plexiglas.nl](http://www.plexiglas.nl/shop/plexiglas-helder-3-mm)|3.50|
|5|1|12V 80 mm Axial Fan|[EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=13244), [Farnell](http://nl.farnell.com/bisonic/sp802512l-03/fan-80x25mm-12vdc/dp/1832326)|8.99|
|6|1|Power switch|[EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=11596), [iPrototype](https://iprototype.nl/products/components/buttons-switches/rocker-switch-large)|0.95|
|7|1|Water proof temperature sensor (*1)|[HobbyElectronica](http://www.hobbyelectronica.nl/product/ds18b20-waterdicht/), [iPrototype](https://iprototype.nl/products/components/sensors/waterproof-tmp-sensor-10k)|2.95|
|8|1|4 section 7 segment display|[EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=8390), [iPrototype](https://iprototype.nl/products/components/led-lcd/led-bar-4xsegment-red)|1.95|
|9|1|MOSFET|[Farnell](http://nl.farnell.com/stmicroelectronics/stp36nf06l/mosfet-n-logic-to-220/dp/9935614?CMP=i-bf9f-00001000), [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=1279)|0.98|
|10|4|10K resistor|[Farnell](http://nl.farnell.com/te-connectivity/cfr16j10k/resistor-carbon-10k-0-25w-5/dp/2329474), [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=13492)|0.06|
|11|1|Diode|[iPrototype](https://iprototype.nl/products/components/overige/diode), [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=10717)|0.19|
|1|1|Chapa de MDF 3mm (cortada 600x400mm)|[Leroy Merlin](http://www.leroymerlin.com.br/mdf-2750-mmx1830-mmx3-mm-5004014-leo-madeiras_88365025)|75.00|
|2|1|Pacote (5un) placas Isopor 100x50x5cm|[Só Isopor](http://www.soisopor.com.br/?1.0.0.0,1170,pacote-placa-50mm-tipo-1.html)|30.00|
|4|1|Chapa de acrílico cristal 3mm (cortada 600x400mm)|[Garagem](-)|15.00|
|5|1|Cooler 80x80x25mm 12V|[Solda Fria](http://www.soldafria.com.br/micro-ventilador-80x80x25hs-12v-sem-rolamento-p-4234.html)|5.74|
|6|1|Chave liga/desliga gangorra 20A|[Solda Fria](http://www.soldafria.com.br/chave-gangorra-kcd2-102n-vermelha-c-neon-e-marcacao-p-4534.html)|1.35|
|7|1|Termistor a prova dágua 10K|[Asla](http://asla.com.br/sensor-temperatura-ntc-10k-inox-ip67.html)|26.00|
|8|1|Display LED segmento quádruplo|[Solda Fria](http://www.soldafria.com.br/display-de-led-vermelho-catodo-quadruplo-d4156ksr-056-p-5992.html)|5.41|
|9|1|MOSFET|[Mouser](http://br.mouser.com/Search/ProductDetail.aspx?R=STP36NF06Lvirtualkey51120000virtualkey511-STP36NF06L)|4.2|
|10|4|Resistor 10K 1/4W|[Solda Fria](http://www.soldafria.com.br/resistor-de-10k-carbono-5-14w-mrptlrdr-p-163.html)|0.05|
|11|1|Diodo 1N4007|[Solda Fria](http://www.soldafria.com.br/diodo-1n4007-p-38.html)|0.10|
|12|2|Button|[iPrototype](https://iprototype.nl/products/components/buttons-switches/momentary-push-button), [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=14721)|0.55|
|13|1|White LED|[iPrototype](https://iprototype.nl/products/components/led-lcd/ledwit), [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=9088)|0.52|
|14|1|220 Ohm resistor|[iPrototype](https://iprototype.nl/products/components/resistors/220R), [EOO](http://www.eoo-bv.nl/index.php?_a=viewProd&productId=5785)|0.45|
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