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NOAA PMEL/UW CICOES
- Sand Point, Seattle, WA
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NOAA-PMEL-OME_Extraction_Sterivex_QIAvac_Protocol_BeBOP
NOAA-PMEL-OME_Extraction_Sterivex_QIAvac_Protocol_BeBOP PublicNucleic acids extraction from the sterivex filters using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol, including the use of a QIAvac. These extracti…
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NOAA-PMEL-OME_eDNA_Collection_Protocol_DiscFilters_VacuumManifold_BeBOP
NOAA-PMEL-OME_eDNA_Collection_Protocol_DiscFilters_VacuumManifold_BeBOP PublicWater collection + EtOH subsampling protocol, plus subsequent filtration using disc filters and a vacuum manifold. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular E…
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NOAA-PMEL-OME_eDNA_Collection_Protocol_Sterivex_GravityBag_BeBOP
NOAA-PMEL-OME_eDNA_Collection_Protocol_Sterivex_GravityBag_BeBOP PublicWater collection and filtration protocol using sterivex filters and gravity bags to collect environmental DNA from marine ecosystems. This collection and filtration protocol is used by the NOAA PME…
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NOAA-PMEL-OME_Organismal_Subsampling_Tissue_Extraction_Protocol_BeBOP
NOAA-PMEL-OME_Organismal_Subsampling_Tissue_Extraction_Protocol_BeBOP PublicOrganismal subsampling and Nucleic acids extraction from the tissue samples using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol. These collection and…
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