BCA Results

This template is to calculate BCA concentrations with set up BSA standards

Library Setup

Please have a check on file "Library.txt" before running for this template If it is first time running for this template Then please install those packages by copy to your R terminal

install.packages(c("readxl", "summarytools", "skimr", "ggplot2"))

You can drag and drop your xlsx file to run.bat if you have setup R and all packages locally. These files will be generated:

• BCA_Graph.jpg
• output.log
• Rplots.pdf

Use run-portable.bat if you have the R installed on a thumb drive. It supports R 4.1.3 on Windows 7 (32 bit) and up.

BSA Standards

In this template, we have 0.05g/l, 0.1g/l, 0.2g/l and 0.4g/l standard to form linear regression models If you are having those four standard concentrations please proceed without modification

96-wells plate setup

In this template, we setup 96-well plate in .xlsx style which is:

A1 for each variables name
A2~C2 for each blanks
A3~C3 for each 0.05g/l standard
A4~C4 for each 0.1g/l standard
A5~C5 for each 0.2g/l standard
A6~C6 for each 0.4g/l standard
Ax~Cx for each unknown proteins

If plate setup followed in that way, then delete all the other empty column The "Sample.xlsx" and "Sample.csv" will be shown in this folder

Notes

Remember that this template is only having two unknown proteins If we are having more than 2 unknown proteins Then you need to add another 'Unk3', 'Unk4 etc.

More than 2 samples

Remember to add in each line that related to 'Unk_x'

Warning

This template is only to use for calculating of BCA results Do not respond for any self-examination of validity of data Please check R-square and p-value themselves in terminal as the command has been added in this template