co-translating_ribosome_scripts

Python codes related to the analysis of co-translating ribosomes on outer mitochondrial membrane for this publication:

Cytoplasmic ribosomes on mitochondria alter the local membrane environment for protein import. Ya-Ting Chang, Benjamin A. Barad, Juliette Hamid, Hamidreza Rahmani, Brian M. Zid, Danielle A. Grotjahn J Cell Biol. (2025) 224 (4): e202407110; doi:https://doi.org/10.1083/jcb.202407110

The development of the scripts was based on the Surface Morphometrics toolkit. Installing Surface Morphometrics is highly recommended.

Citation

Pycurv and Surface Morphometrics toolkits were applied to this project.

Surface morphometrics: https://github.com/GrotjahnLab/surface_morphometrics

Quantifying organellar ultrastructure in cryo-electron tomography using a surface morphometrics pipeline.
Benjamin A. Barad^†, Michaela Medina^†, Daniel Fuentes, R. Luke Wiseman, Danielle A. Grotjahn
J Cell Biol. 2023 Apr 3;222(4):e202204093; doi: https://doi.org/10.1083/jcb.202204093

Pycurv: https://github.com/kalemaria/pycurv

Reliable estimation of membrane curvature for cryo-electron tomography.
Maria Salfer,Javier F. Collado,Wolfgang Baumeister,Rubén Fernández-Busnadiego,Antonio Martínez-Sánchez
PLOS Comp Biol August 2020; doi: https://doi.org/10.1371/journal.pcbi.1007962