Analysis of proteomic data from single myofiber protein function omic experiments. In the case of this repository, data is derived from healthy human (N=8) and nemaline myopathy patients carrying mutations in ACTA1 (N=3) and TNNT1 (N=3). Single myofibers are cleaved from SkM biopsies and used to co-profile the conformational behaviour of myosin heads (DRX vs SRX relaxation state) and cell-wide proteomics.
There are two subdirectories, containing scripts for analyses conducted in Figure 1 and Figure 2 of the associated manuscript.
Analyses conducted in R Studio Version 2024.04.1+748 (2024.04.1+748). Raw data is available in PRIDE repository under the accession: