Releases: SAMtoBAM/PAQman
V1.2.0
Coverage:
- Changes Filtlong downsampling for faster Rasusa (and now purely random)
- Uses downsampled reads for coverage analysis to speed up alignment phase (default: maximum 30X coverage)
- Option to set fold coverage parameter --coveragemax
- Skip downsampling by setting --coveragemax to 0
- Use Mosdepth for calculating coverage from alignments (and removing need to resort)
Meryl/Merqury:
- Adds user-modifiable parameters for meryl (--merylkmer and --merylmem)
- Adds ability to input local meryl database (--meryldb)
Adds option to input local BUSCO database (--localbuscodb)
CRAQ/Correctness:
- Reduced mapping redundancy by proving CRAQ with pre-aligned reads also used for coverage analysis (and checked for consistency with default CRAQ alignment)
- Post-installation edits of CRAQ scripts (runSR.sh and runLR.sh) to add multithreading during
samtools depth
Adds --resume option for runs
Can now use regular expressions as input for --telomererepeat
Add memory per thread option during samtools sort (--sortmem)
Quast is now multithreaded
Added multithreaded pigz compression after downsampling
v1.1.0
Updated conda package to include all R packages including ggradar and removing installation check during script run
Adding --sequences option to disitnguish between assemblies to be kept scaffolded or broken into contigs for all subsequent analyses. Previously it was a mix of both scaffolds and contigs.
Fixed paqplot issues with library installation
Fixed paqplot issues with variables being plotted
Modified paqplot variable for plotting (e.g. remove underscores for the plots, change labelling scheme)
v1.0.0-beta
Version 1 release of PAQman
Contains both functions paqman.sh and paqplot.sh
v0.0.1-beta
Zenodo reference release
Added Apptainer/singularity image
Added coverage_normal(%) stat to paqman output
Updated paqplots to handle coverage_normal(%) stat