updating branch

.github/ISSUE_TEMPLATE/new-tool-method-entry.yml

@@ -2,7 +2,7 @@ name: New Method Catalogue Entry
 description: Request to add a new method to the catalogue
 title: "[METHOD]: "
 labels: ["method"]
-assignees: jmillanacosta
+assignees: ShakiraPortfolio
 
 body:
   - type: markdown

README.md

@@ -3,6 +3,10 @@
 Repository to document the services on the VHP4Safety Cloud, see this [index](docs/index.md)
 or the [online website](https://vhp4safety.github.io/cloud/).
 
+## Adding new methods
+* Post an issue requesting a new method
+* For maintainers: add the [`method-approved-for-pr` label](https://github.com/VHP4Safety/cloud/issues?q=is%3Aissue%20state%3Aopen%20label%3Amethod-approved-for-pr) when the generated JSON looks right.
+
 ## Adding new services
 
 * copy the `docs/services/template.json` file and adapt the content to the new service

docs/methods/index.md

@@ -8,7 +8,7 @@ This catalog contains experimental methods and protocols used in VHP4Safety rese
 
 [Request a new method](https://github.com/VHP4Safety/cloud/issues/new/choose) | [Browse all methods](https://github.com/VHP4Safety/cloud/tree/main/docs/methods)
 
-## Available Methods (18)
+## Available Methods (19)
 
 ### AOP Methods
 
@@ -44,6 +44,10 @@ mitochondrial membrane potential assay (Vendor: Thermofisher) [[more information
 
 Fluorescent-based assay to label mitochondria (Vendor: ThermoFisher Scientific) [[more information](mitotracker_deep_red_fm_assay.md)]
 
+#### Resazurine assay
+
+Fluorometric method to measure cell viability through enzymatic reduction of resazurin to resorufin by viable cells (Vendor: ThermoFisher Scientific) [[more information](resazurine_assay.md)]
+
 ### AOP, Adverse Outcome Methods
 
 #### Cytotoxicity Detection KitPLUS (LDH)

docs/methods/resazurine_assay.json

@@ -0,0 +1,25 @@
+{
+  "id": "resazurine_assay",
+  "method": "Resazurine assay",
+  "issue_number": 243,
+  "assay_name_content": "Resazurine assay",
+  "data_producer_content": "Unknown at this timepoint",
+  "available_sop_or_protocol_content": "_No response_",
+  "method_description_content": "Fluorometric method to measure cell viability through enzymatic reduction of resazurin to resorufin by viable cells",
+  "case_study_content": "kidney",
+  "http": "//purl.obolibrary.org/obo/OBI_0003757",
+  "ontology_term_url": "http://purl.obolibrary.org/obo/OBI_0003757",
+  "regulatory_question_content": "Q2",
+  "type_content": "ontology",
+  "relevant_vhp4safety_regulatory_question(s)_content": "Kidney Disease (b)",
+  "relevant_aop_wiki_key_event(s)_to_the_assay_content": "_No response_",
+  "relevant_aop_wiki_adverse_outcome_pathway(s)_to_the_assay_content": "_No response_",
+  "vendor_content": "ThermoFisher Scientific",
+  "catalog_number_content": "V23110",
+  "https": "//documents.thermofisher.com/TFS-Assets/LSG/manuals/mp23110.pdf",
+  "catalog_webpage_url": "https://www.thermofisher.com/order/catalog/product/V23110?SID=srch-srp-V23110",
+  "citation_url": "https://documents.thermofisher.com/TFS-Assets/LSG/manuals/mp23110.pdf",
+  "vhp4safety_workflow_stage_content": "AOP",
+  "workflow_substage_content": "Effects on Key Events",
+  "timestamp": "2026-03-09T09:56:34.458233Z"
+}

docs/methods/resazurine_assay.md

@@ -0,0 +1,45 @@
+# Resazurine assay
+
+<!--- This file is autogenerated. Edit resazurine_assay.json to make changes in this page. --->
+
+Fluorometric method to measure cell viability through enzymatic reduction of resazurin to resorufin by viable cells
+
+## Method Details
+
+* **Vendor:** ThermoFisher Scientific
+
+* **Catalog Number:** V23110
+
+* **Catalog Webpage:** [https://www.thermofisher.com/order/catalog/product/V23110?SID=srch-srp-V23110](https://www.thermofisher.com/order/catalog/product/V23110?SID=srch-srp-V23110)
+
+* **VHP4Safety Workflow Stage:** AOP
+
+* **Workflow Substage:** Effects on Key Events
+
+* **Case Study:** kidney
+
+* **Regulatory Question:** Q2
+
+## Additional Information
+
+* **Assay Name:** Resazurine assay
+
+* **Data Producer:** Unknown at this timepoint
+
+* **Relevant AOP Wiki Key Event(s):** _No response_
+
+* **Relevant AOP Wiki Adverse Outcome Pathway(s):** _No response_
+
+* **Available SOP or Protocol:** _No response_
+
+* **Ontology Term:** Not available
+
+* **Citation:** Not available
+
+---
+
+*Generated from issue #243*
+
+<script>
+(async()=>{document.querySelectorAll('li').forEach(li=>{if(li.textContent.includes('Ontology Term:')&&li.textContent.includes('No response')){const a=li.querySelector('a');if(a){const span=document.createElement('span');span.textContent='No ontology annotation';a.replaceWith(span)}}});const e=encodeURIComponent,t=document.querySelectorAll('a[href^="http://purl"]');for(const r of t){const t=r.closest('li');if(t?.textContent?.includes("Ontology Term:")){const o=r.href;try{const t=await fetch(`https://www.ebi.ac.uk/ols4/api/terms/${e(e(o))}?lang=en`),n=await t.json(),a=n._embedded?.terms?.[0];a&&(r.textContent=a.short_form?`${a.label} (${a.short_form})`:a.label,r.href=a._links.self.href)}catch(e){}await new Promise((e=>setTimeout(e,100)))}}})();
+</script>