Why multiple aligners are used here?

[vivekruhela]

I think any one alignment tool like BWA ,Bowtie etc gives us sufficient details about alignment of exome sample to reference genome but I am getting why multiple tools are used here because it will be then more computationally complex. Will it give better alignment score of resultant file. Have you merged all the SAM files obtained after difference alignment tool operation? Thanks.

[yunfeiguo]

Hi, Yes, you are right. Each aligner by itself is pretty good, however, variants called on alignments by different aligners may differ a lot (see SeqMule paper). Thanks.

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On Mar 9, 2018, at 1:20 AM, vivekruhela ***@***.***> wrote: I think any one alignment tool like BWA ,Bowtie etc gives us sufficient details about alignment of exome sample to reference genome but I am getting why multiple tools are used here because it will be then more computationally complex. Will it give better alignment score of resultant file. Have you merged all the SAM files obtained after difference alignment tool operation? Thanks. — You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub <#170>, or mute the thread <https://github.com/notifications/unsubscribe-auth/AHYPztXDxHA_Uxtg_aiPS-WJXuEWU_bpks5tcklqgaJpZM4Sj-QD>.