Regulation of the activity of proteins enables control of complex cellular processes. This platform enables the construction of ON/OFF protein switches, their regulation by small molecules, and Boolean logic functions with a rapid response in mammalian cells.
This repository contains the code to predict optimal regulatory insertion sites. Structures containing regulatory coiled-coils are also predicted using AlphaFold2. The code is also available as a colab notebook.
If you use this software please cite: Plaper, Merljak, Fink et al, Cell Discovery, 2024, https://www.nature.com/articles/s41421-023-00635-y