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Automating processes on the Illumina HiSeq X, based on code used for the HiSeq 2500.

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PySeqX

Automating processes on the Illumina HiSeq X, based on code used for the HiSeq 2500.

Background: The HiSeq is an instrument used for DNA sequencing. It was produced by Illumina, who have recently discontinued this device in favor of newer sequencing technology. The HiSeq device has a 3-axis motorized stage (x-stage, y-stage, and z-stage), several methods of temperature control including a fluidics system, an integrated fluidics system, and TDI CCD cameras.

Project Goal: PySeqX is an open-source code that controls the HiSeq X device and automates additional HiSeq X applications that go beyond initial sequencing applications. PySeq2500 is an open-souce code that already fulfills this purpose for the HiSeq 2500/2000 device, which are predecessors to the HiSeq X device. However, the HiSeq X differs from the HiSeq 2500 in a number of ways, hence the need for updated code. The HiSeq X has a different driver for the Y stage, a different fluidics system, and a different driver for the CCD camera.

PySeq2500: Kunal's code Richard's code

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Automating processes on the Illumina HiSeq X, based on code used for the HiSeq 2500.

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