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🧬 Mediterranean Diet & Neuroinflammation Gene Expression (PREDIMED Substudy)

This repository contains R scripts and supplementary materials related to the data preprocessing and analysis of the study:

"Mediterranean Diet Modulation of Neuroinflammation-Related Genes in Elderly Adults at High Cardiovascular Risk"
Published in Nutrients (2024), 16, 3147.
🔗 DOI: 10.3390/nu16183147


📖 Study Purpose

This project explores the transcriptional impact of two traditional Mediterranean Diet (MedDiet) interventions—one enriched with extra-virgin olive oil (EVOO) and the other with Nuts—on gene expression related to neuroinflammatory pathways and cardiovascular risk. The analyses were performed using data from a subsample (n = 134) of the larger PREDIMED trial.

We evaluated key inflammation-related genes (e.g., CDKN2A, IFNG, TGFB2, NAMPT, NLRP3) at baseline and after a 12-month intervention using qRT-PCR techniques.

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