Welcome to the BSATOS wiki! bulked-segregant analyis tools for outbreeding species(BSATOS) is developed by the institute for horticultue plants, China Argicultural University and Edmund Mach Foundation, Italy. BSATOS is designed for NGS-based Bulked-segregant analysis for outbreeding species, includings fruits trees, such as apple, and cirtus.
To improve the gene mapping efficiency of next generation sequencing-based segregant analysis (BSA) in outbreeding species and realize the rapid candidate gene mining based on multi-omics data, bulked segregant analysis tools for outbreeding species (BSATOS) was developed. Different from the classic two-way pseudo-testcross (PT) strategy, Local phased 3-way BSA mapping strategy was introduced to use three type of segregate makers unbiased. After comparing different statistical methods to evaluate the allele frequency difference, G value method was selected. Multi-round G value screening and multi-omics data based gene mining were also integrated.
Introduction and Quick start:
https://github.com/maypoleflyn/BSATOS/wiki
Installation:
https://github.com/maypoleflyn/BSATOS/wiki/Installation
Commands:
https://github.com/maypoleflyn/BSATOS/wiki/Commands
Examples:
https://github.com/maypoleflyn/BSATOS/wiki/Examples
Documentation：
https://drive.google.com/file/d/1gPjisojwkKkCgLNS0fu3uqafwUp_o7Vm/view?usp=sharing
Citation:
Fei Shen et al, A bulked segregant analysis tool for out-crossing species (BSATOS) and QTL-based genomics-assisted prediction of complex traits in apple, Journal of Advanced Research, 2022, 2090-1232, https://doi.org/10.1016/j.jare.2022.03.013.
Please contact Fei Shen (shenf1028@gmail.com), if you have any question.