EasyFRAP assists quantitative and qualitative analysis of FRAP data. The user can handle simultaneously large datasets of raw data, visualize fluorescence recovery curves, exclude low quality data, perform data normalization, extract quantitative parameters, perform batch analysis and save the resulting data and figures for further analysis. EasyFRAP is implemented as a single-screen Graphical User Interface and is highly interactive, as it permits parameterization and visual data quality assessment at various points in the analysis. EasyFRAP was developed by the Cell Cycle Lab of the University of Patras and is free software, available under the General Public License version 3 (GPL v3).
For updates regularly check out our lab's webpage!
If you use our tool please cite:
Rapsomaniki MA, Kotsantis P, Symeonidou IE, Giakoumakis NN, Taraviras S and Lygerou Z (2012). easyFRAP: an interactive, easy-to-use tool for qualitative and quantitative analysis of Fluorescence Recovery After Photobleaching data. Bioinformatics. 28(13):1800-1801 (https://doi.org/10.1093/bioinformatics/bts241)
See also:
EasyFRAP-web: EasyFRAP became web-based! Visit EasyFRAP web here or read our paper:
Koulouras G, Panagopoulos A, Rapsomaniki MA, Giakoumakis NN, Taraviras S, Lygerou Z (2018). EasyFRAP-web: a web-based tool for the analysis of fluorescence recovery after photobleaching data. Nucleic Acids Research, Volume 46, Issue W1, 2 July 2018, Pages W467–W472, (https://doi.org/10.1093/nar/gky508)
Stochastic modeling of FRAP experiments: For inference of kinetic parameters from FRAP data (diffusion coefficient, bound fraction and residence time) see:
Rapsomaniki MA, Cinquemani E, Giakoumakis NN, Kotsantis P, Lygeros J, & Lygerou Z (2015). Inference of protein kinetics by stochastic modeling and simulation of fluorescence recovery after photobleaching experiments. Bioinformatics, 2015 Feb 1;31(3):355-62. (https://doi.org/10.1093/bioinformatics/btu619)