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Kallisto

kallisto is a program for quantifying abundances of transcripts from bulk and single-cell RNA-Seq data, or more generally of target sequences using high-throughput sequencing reads.

Input data

In data directory, tool searches for .fastq.gz sequencing files and .fasta reference sequences.

Parameters

Main Parameters

Number of Threads

  • Number of cores used by featureCounts. If not specified, will use all available.

Type of Sequencing

  • Single or Paired Ended reads

Length of Fragment

Standard Deviation of Fragment

Number of Bootstraps k > 0

Kmer Size for DeBruijn Graph Int < 31 (Odd)

Prefix for Bus File

Chemistry Type - See Drop Down Menu

Source

https://pachterlab.github.io/kallisto/about

Output

.tsv: Output Counts Matrix .h5 .json Logging information

About

a tool for creating BUS files.

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