Skip to content

Very high ambient RNA detected #253

New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

Sign up for GitHub

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Jump to bottom
Open
deepalivasoya opened this issue Mar 13, 2025 · 0 comments
Open

Very high ambient RNA detected #253

deepalivasoya opened this issue Mar 13, 2025 · 0 comments

Comments

@deepalivasoya
Copy link

I have been running souporcell on 20 samples and all of these samples have two individuals pooled. For 14 samples I have noticed very high ambientENA estimated (>95%). The barcodes were still split into two individuals and doublets and unassigned were detected within normal range. But with this high ambient RNA estimation, I was confused whether to trust the demultiplexed results. Can you explain or guide me what could be going on here? Thanks.
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Assignees
No one assigned
Labels
None yet
Projects
None yet
Milestone
No milestone
Development

No branches or pull requests
1 participant
@deepalivasoya