Download and install:
bowtie (not bowtie2)
django 1.9.5
python 2.7.9
biopython (python module)
In crispr_app/views.py, change all user specific references to bowtie to your directory of bowtie. In crispr_app/functions.py, change all user specific references to crispr_app/Rule_Set_2_scoring_v1/analysis to your own directory with the analysis executable.
Navigate to the parent directory in the terminal and run "python manage.py runserver".
Navigate to the default web page, given in the terminal. The default web page should be "http://127.0.0.1:8000". Once there, enter a gene symbol of interest and a CRISPR analysis category. Alternatively, enter a DNA sequence of appropriate length (>30bp) with at least one PAM sequence (NGG). Then, once the gene or sequence name has been entered, hit "Search" to process the data. Notes: The default gene loaded into the SQLite database is "APOB", and should return results quickly, and without processing any alignments.