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Rasmus Hammar edited this page Apr 10, 2026
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RNA-seq and Tn-seq reveal fitness determinants of vancomycin-resistant Enterococcus faecium during growth in human serum
Species is a vancomycin resistant strain of Enterococcus faecium (E. faecium)
https://www.ebi.ac.uk/ena/browser/view/prjeb19025
Identify genes in E. faecium relevant for the adaptation to growing in human serum.
Genes of interest found in E. faecium according to the paper.
| Gene | Description |
|---|---|
| manZ_3 | Mannose PTS permease subunit (sugar transport) |
| manY_2 | Mannose PTS permease subunit (sugar transport) |
| ptsL | Phosphotransferase system (PTS) component (sugar uptake) |
| algB | Response regulator / transcriptional regulator |
| guaB | IMP dehydrogenase (purine biosynthesis) |
| purA | Adenylosuccinate synthase (purine biosynthesis) |
| pyrF | Orotidine 5'-phosphate decarboxylase (pyrimidine biosynthesis) |
| pyrK_2 | Dihydroorotate dehydrogenase-related subunit (pyrimidine biosynthesis) |
| purD | Phosphoribosylamine–glycine ligase (purine biosynthesis) |
| purH | AICAR transformylase/IMP cyclohydrolase (purine biosynthesis) |
| purL | Phosphoribosylformylglycinamidine synthase (purine biosynthesis) |
| purQ | Phosphoribosylformylglycinamidine synthase subunit (purine biosynthesis) |
| purC | SAICAR synthase (purine biosynthesis) |
| clsA_1 | Cardiolipin synthase (membrane lipid biosynthesis) |
| ddcP | D-alanyl-D-alanine carboxypeptidase / cell wall remodeling |
| ldt_fmp | L,D-transpeptidase (peptidoglycan cross-linking) |
| mgs | Methylglyoxal synthase (glycolytic side-path) |
| lytA_2 | Autolysin (peptidoglycan hydrolase) |
Uses PacBio long reads assembled with Canu.
RNA-seq from two culture conditions, BHI and human serum.