Main repository for NOAA Pacific Marine Environmental Laboratory Ocean Molecular Ecology group bioinformatics and data analysis.

Collection of biomolecular protocol templates for an organization

Minimum Information about an Omics Protocol (MIOP) specifications

Protocols used by AOML Omics labs, following the BeBOP-OBON standard

Protocols for sampling, processing, and analysis of eDNA collected on CalCOFI cruises. All protocols are formatted according to guidelines from Better Biomolecular Ocean Practices (BeBOP) using Minimum Information about an Omics Protocol (MIOP) terms.

In lab and out of lab protocols for MBON eDNA collection and processing

Water collection and filtration protocol using sterivex filters to collect environmental DNA from marine ecosystems. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.

Protocol for extracting DNA using phenol-chloroform and isopropanol at Smithsonian NMNH

Water collection + EtOH subsampling protocol, plus subsequent filtration using disc filters and a vacuum manifold. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.

This protocol is for running and imaging PCR product using gel electrophoresis.

Water collection and filtration protocol using sterivex filters and gravity bags to collect environmental DNA from marine ecosystems. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.

This protocol is for quantifying the concentration of DNA in the product of a DNA extraction.

Water collection and filtration protocol using Smith-Root self preservng eDNA filters to collect environmental DNA from marine ecosystems. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.

BeBOP protocol for secondary amplification, size selection, and sequencing at Michigan State University for Illumina MiSeq.

Protocol for extracting DNA from sediment trap samples using the Qiagen PowerSoil Pro Kit

Protocol for preparing COI (Leray-Geller) amplicon libraries at Smithsonian NMNH

Protocol for preparing metagenomic libraries using Illumina DNA Prep